|Application ||IHC-F, IF, E|
|Other Accession||P02671, P02675|
|Calculated MW||51512 Da|
|Purification||Purified IgG prepared by ion exchange chromatography|
|Immunogen||Human fibrinogen purified from plasma.|
|Shelf Life||18 months from the date of despatch.|
|Other Names||Fibrinogen gamma chain, FGG|
|Target/Specificity||Sheep anti-Human fibrinogen antibody recognizes human fibrinogen, a complex ~340kDa hetero-hexameric (di-trimeric) glycoprotein consisting of 3 pairs of α, β and γ chains linked by a series of 29 disulphide bonds (Henschenet al.1983). The six chains are arranged in such a way that all the N-Terminal ends adjoin to form a central E domain with two trimeric coiled coil structures connecting to outer D domains. Fibrinogen plays an important role in the coagulation process with the D and E domains interacting via the C-Terminal ends of the α chains during fibrin clot cross-linking. Sheep anti-human fibrinogen antibody shows minimal cross-reactivity with related serum proteins. Fibrinogen has been identified as a ferritin binding protein in the horse (Orinoet al.1993). Sheep anti-human fibrinogen antibody has been successfully as a capture reagent for ferritin - anti-ferritin IgG complexes in horse plasma to evaluate the antibody response to ferritin by ELISA (Takahashiet al.2013).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Fibrinogen Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Brill, A. et al. (2011) von Willebrand factor-mediated platelet adhesion is critical for deep vein thrombosis in mouse models.Blood. 117: 1400-7. 2. Barrera, V. et al. (2011) Host fibrinogen stably bound to hemozoin rapidly activates monocytes via TLR-4 and CD11b/CD18-integrin: a new paradigm of hemozoin action.Blood. 117: 5674-82. 3. Grainger, D.J. et al. (2004) Apolipoprotein E modulates clearance of apoptotic bodies in vitro and in vivo, resulting in a systemic proinflammatory state in apolipoprotein E-deficient mice.J Immunol. 173: 6366-75. 4. Grainger, D.J. et al. (2001) Suppressing Thrombin Generation is Compatible With the Development of Atherosclerosis in MiceThromb Res. 102: 71-80. 5. Chien, H.W. (2013) Surface conjugation of zwitterionic polymers to inhibit cell adhesion and protein adsorption.Colloids Surf B Biointerfaces. 107: 152-9. 6. Plskova, J. et al. (2004) Quantitative evaluation of the corneal endothelium in the mouse after grafting.Br J Ophthalmol. 88: 1209-16. 7. Takahashi, K. et al. (2013) The presence of heat-labile factors interfering with binding analysis of fibrinogen with ferritin in horse plasma.Acta Vet Scand. 55: 70. 8. Ozaltin, F. et al. (2013) DGKE variants cause a glomerular microangiopathy that mimics membranoproliferative GN.J Am Soc Nephrol. 24: 377-84. 9. Dmitrieva, N.I. and Burg, M.B. (2014) Secretion of von Willebrand factor by endothelial cells links sodium to hypercoagulability and thrombosis.Proc Natl Acad Sci U S A. 111: 6485-90. 10. Johnsen, D. et al. (2015) Disrupting protein tyrosine phosphatase σ does not prevent sympathetic axonal dieback following myocardial infarctionExperimental Neurology. Nov 23 [Epub ahead of print]1. Kamath, S. and Lip, G.Y. (2003) Fibrinogen: biochemistry, epidemiology and determinants. Q.J.M. 96: 711-729.2. Mosesson, M.W. (2005) Fibrinogen and fibrin structure and functions. J. Thromb. Haemost. 3: 1894-1904.
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