|Application ||WB, IHC-P, IHC-F, IF, IP|
|Other Accession||P14672, P14142|
|Calculated MW||54896 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.|
|Immunogen||Partially purified vesicles containing insulin-responsive glucose transporter 4.|
|Shelf Life||18 months from the date of despatch.|
|Other Names||Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive, GLUT-4, Slc2a4, Glut-4, Glut4|
|Target/Specificity||Mouse anti-glucose transporter 4 antibody, clone 1F8 originally raised against rat intracellular low density microsomes (Jameset al.1987) recognizes an epitope in the cytoplasmic region of Glucose transporter 4 (GLUT4), an insulin-regulated facilitative glucose transporter found in adipose tissue and striated muscle. When stimulated by insulin, GLUT4 translocates from intracellular stores to the cell surface, facilitating passive diffusion of circulating glucose into muscle and fat cells. GLUT4 is also stimulated to locate to the cell surface by muscle contraction, particularly in cardiac muscle (Jameset al.1988).Mouse anti-glucose transporter 4 antibody, clone 1F8 has been used successfully to demonstrate the localization of GLUT4 to the basolateral side of ductal structures in the rat submandibular salivary gland in formalin fixed, paraffin embedded material (Cetiket al.2014).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Glucose Transporter 4 Antibody, clone 1F8 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. James, D.E. et al. (1988) Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein.Nature. 333 (6169): 183-5. 2. James, D.E. et al. (1989) Molecular cloning and characterization of an insulin-regulatable glucose transporter.Nature. 338 (6210): 83-7. 3. Cleasby, M.E. et al. (2003) Programming of rat muscle and fat metabolism by in utero overexposure to glucocorticoids.Endocrinology. 144 (3): 999-1007. 4. Huang, J., et al. (2001) Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein. Proc Natl Acad Sci U S A. 98:13084-13089. 5. Farese, R.V. et al. (2007) Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.J Clin Invest. 117: 2289-301. 6. Grainger, D.L. et al. (2011) Involvement of phosphatidylinositol 5-phosphate in insulin-stimulated glucose uptake in the L6 myotube model of skeletal muscle.Pflugers Arch. 462: 723-32. 7. Minakawa, M. et al. (2011) Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic ß-cells.J Clin Biochem Nutr. 48: 237-44. 8. Gillies, R.M. et al. (2011) Immunohistochemical assessment of intrinsic and extrinsic markers of hypoxia in reproductive tissue: differential expression of HIF1α and HIF2α in rat oviduct and endometrium.J Mol Histol. 42: 341-54. 9. Aksentijević, D. et al. (2009) Insulin resistance and altered glucose transporter 4 expression in experimental uremia.Kidney Int. 75: 711-8. 10. Imamura, T. et al. (2001) beta -Arrestin-mediated recruitment of the Src family kinase Yes mediates endothelin-1-stimulated glucose transport.J Biol Chem. 276 (47): 43663-7. 11. Allard, M.F. et al. (2000) Hypertrophied rat hearts are less responsive to the metabolic and functional effects of insulin.Am J Physiol Endocrinol Metab. 279 (3): E487-93. 12. Park, S. K. et al. (2009) Chronic activation of 5'-AMP-activated protein kinase changes myosin heavy chain expression in growing pigs.J Anim Sci. Oct;87(10):3124-33 13. Lalioti, V.S. et al. (2002) The insulin-sensitive glucose transporter, GLUT4, interacts physically with Daxx. Two proteins with capacity to bind Ubc9 and conjugated to SUMO1.J Biol Chem. 277: 19783-91. 14. Spargo, F.J. et al. (2007) Dysregulation of muscle lipid metabolism in rats selectively bred for low aerobic running capacity.Am J Physiol Endocrinol Metab. 292: E1631-6. 15. Cetik, S. et al. (2014) Expression and Localization of Glucose Transporters in Rodent Submandibular Salivary Glands.Cell Physiol Biochem. 33: 1149-1161. 16. de Laat, M.A. et al. (2015) AICAR administration affects glucose metabolism by upregulating the novel glucose transporter, GLUT8, in equine skeletal muscle.Vet J. 205 (3): 381-6.2. Berger, J. et al. (1989) Decreased expression of the insulin-responsive glucose transporter in diabetes and fasting.Nature. 340 (6228): 70-2.
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