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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Anti-Human Plasminogen Antibody (HRP)   

Anti-Human Plasminogen Antibody (FITC)

Sheep Anti-Human Polyclonal Antibody

     
  • E - Anti-Human Plasminogen Antibody (FITC) ABD11901
    Published customer image:Sheep anti Human plasminogen antibody used to detect binding of human plasminogen to the outer envelope of Francisella tularensis by immunogluorescence.Image caption:PLG binds to the outer envelope of FT. Laser scanning confocal microscopy of PLG-associated FTLVS. Bound huPLG ligand was detected using sheep anti-human PLG antibody followed by incubation with Dylight-488 conjugated donkey, anti-sheep/goat IgG secondary antibody. Samples were visualized using a Zeiss LSM 510 confocal microscope.From: Clinton SR, Bina JE, Hatch TP, Whitt MA, Miller MA. Binding and activation of host plasminogen on the surface of Francisella tularensis.BMC Microbiol. 2010 Mar 12;10:76.
  • WB - Anti-Human Plasminogen Antibody (FITC) ABD11901
    Published customer image:Sheep anti Human plasminogen antibody used for the detection of plasminogen in Jurkat cell lysates by western blotting.Image caption:Necrotic cells harbour misfolded proteins and generate surface-bound plasmin which increases their phagocytosis by human MoDCs. (A) Uninjured and necrotic Jurkat lymphocytes were stained with 10 mg/L 7AAD and 10 mg /L Thiazine Red for 15 min then subjected to flow cytometry. (B) The Triton-insoluble fractions of uninjured and necrotic Jurkat lymphocytes were subjected to SDS-PAGE under reducing conditions and subsequent Coomassie staining. (C) Uninjured and necrotic Jurkat lymphocytes were incubated with t-PA and/or plasminogen for 15 min and washed. Total cellular protein lysates were prepared and subjected to SDS-PAGE under reducing conditions and subsequent immunoblot analysis. Most of the t-PA used was in single-chain form (~70 kDa), however a small extent of two-chain t-PA was also apparent (the band indicated by the asterisk at ~35 kDa). (D) PKH67-labelled necrotic Jurkat lymphocytes were treated with the indicated reagents for 15 min, then washed and incubated with PKH26-labelled human MoDCs. 24 h later, the proportion of double-positive (PKH67positive and PKH26positive) MoDCs was assessed by flow cytometry. Data are displayed as fold-change in double-positive MoDCs (mean ± s.e.m.; n = 3–5 independent experiments). Data was normalized to the group where necrotic cells received no exogenous reagent. **p<0.01 and ***p<0.001 by 1-way ANOVA with Newman-Keuls post-hoc analysis.From: Borg RJ, Samson AL, Au AE-L, Scholzen A, Fuchsberger M, Kong YY, et al. Dendritic Cell-Mediated Phagocytosis but Not Immune Activation Is Enhanced by Plasmin.PLoS ONE 10(7): e0131216.
  • WB - Anti-Human Plasminogen Antibody (FITC) ABD11901
    Published customer image:Sheep anti Human plasminogen antibody used to detect binding of human plasminogen to the outer envelope of Francisella tularensis by western blotting.Image caption:Identification of putative PLG-binding proteins of FT. Sarkosyl-soluble and insoluble protein fractions of FTLVS were separated by SDS-PAGE and transferred to PVDF membrane. Membranes were then blotted with huPLG (3 ug/mL) followed by anti-PLG antibody and HRP-conjugated secondary antibody to detect PLG-binding proteins (Panel A). Protein bands on an identical Coomassie Blue-stained SDS-PAGE gel corresponding to those identified via blotting (Panel B) were excised and identified using proteomic methodologies (Panel C).BMC Microbiol. 2010 Mar 12;10:76.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF
Primary Accession P00747
Reactivity Human
Host Sheep
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 90569 Da
Additional Information
Other Species Cat,Rat,Dog,B,Gp
Purification Antisera to human plasminogen were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen Native, highly purified.
Shelf Life 18 months from the date of despatch.
Gene ID 5340
Other Names Plasminogen, 3.4.21.7, Plasmin heavy chain A, Activation peptide, Angiostatin, Plasmin heavy chain A, short form, Plasmin light chain B, PLG
Target/Specificity Sheep anti-Human plasminogen antibody recognizes human plasminogen, a ~90 kDa single-chain glycoprotein and inactive precursor (zymogen) of plasmin, secreted by the liver and present in the blood and majority of extravascular body fluids.Plasminogen is the central component in the fibrinolytic system, and is converted into active plasmin through cleavage by the endogenous activators urokinase (u-PA) and tissue plasminogen activator (t-PA), in the presence of fibrin. The level of plasminogen is maintained in the plasma at around 200mg/L, with an increase being associated with hypothyroidism, obesity and pregnancy, and deficiencies with many disorders including thrombosis, liver disease, sepsis and hyperthyroidism.Sheep anti-Human plasminogen antibody has not been cross adsorbed and may cross react with plasminogen from other species.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Human Plasminogen Antibody (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Clinton, S.R. et al. (2010) Binding and activation of host plasminogen on the surface of Francisella tularensis.
BMC Microbiol. 10:76. 2. Samson, A.L. et al. (2009) A nonfibrin macromolecular cofactor for tPA-mediated plasmin generation following cellular injury.
Blood. 114: 1937-46. 3. Samson, A.L. et al. (2008) Tissue-type plasminogen activator requires a co-receptor to enhance NMDA receptor function.
J Neurochem. 107: 1091-101. 4. Singh, B. et al.(2015)Moraxella catarrhalis binds plasminogen to evade host innate immunity.
Infect Immun. pii: IAI.00310-15. 5. Borg, R.J. et al. (2015) Dendritic Cell-Mediated Phagocytosis but Not Immune Activation Is Enhanced by Plasmin.
PLoS One. 10 (7): e0131216.4. Fears, R. (1989) Binding of plasminogen activators to fibrin: characterization and pharmacological consequences.
Biochem. J. 261: 313-324.

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