|Application ||WB, E|
|Calculated MW||25896 Da|
|Purification||Antisera to mouse TNFα were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Recombinant mouse TNFα(PMP24)|
|Shelf Life||6 months from date of reconstitution.|
|Other Names||Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnf, Tnfa, Tnfsf2|
|Target/Specificity||Rabbit anti-Mouse TNF alpha antibody recognizes mouse Tumour Necrosis Factor alpha (TNF–alpha), a 17.3kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.|
|Precautions||Anti-Mouse TNF alpha Antibody (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Khaskhely, N.M. et al. (2002) Low-dose UVB contributes to host resistance against Leishmania amazonensis infection in mice through induction of gamma interferon and tumor necrosis factor alpha cytokines.Clin Diagn Lab Immunol. 9: 677-86. 2. Schafers, M. et al. (2002) Anterograde transport of tumor necrosis factor-alpha in the intact and injured rat sciatic nerve.J Neurosci. 22: 536-45. 3. Oakley, F. et al. (2005) Nuclear factor-kappaB1 (p50) limits the inflammatory and fibrogenic responses to chronic injury.Am J Pathol. 166: 695-708.1. Van Horssen, R. et al. (2006) TNF-alpha in cancer treatment: molecular insights, antitumor effects, and clinical utility.The Oncologist. 11: 397-408.
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