|Application ||WB, IHC-P, E, Func|
|Calculated MW||25806 Da|
|Purification||Antisera to rat TNF alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Recombinant rat TNF alpha (PRP16).|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnf, Tnfa, Tnfsf2|
|Target/Specificity||Rabbit anti-Rat TNF alpha antibody recognizes rat tumor necrosis factor alpha (TNFα), also known as Cachectin or Tumor necrosis factor ligand superfamily member 2. Rat TNFα is a 235 amino acid ~17 kDa single pass type II O-linked transmembrane glycoprotein, it also exists as a secreted cytokine through cleavage between residues 79 and 80. TNFα is produced by many cell types including macrophages, monocytes, neutrophils and a population of activated NK cells.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.|
|Precautions||Anti-Rat TNF alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Halin, S. et al. (2010) Pigment epithelium-derived factor stimulates tumor macrophage recruitment and isdownregulated by the prostate tumor microenvironment.Neoplasia. 12: 336-45. 2. Khandelwal, P.J. et al. (2010) Parkinson-related parkin reduces a-Synuclein phosphorylation in a gene transfer model.Mol Neurodegener. 5: 47. 3. Sandoval, J. et al. (2010) Ordered transcriptional factor recruitment and epigenetic regulation of tnf-alpha in necrotizing acute pancreatitis.Cell Mol Life Sci. 67: 1687-97. 4. Irwin, M.W. et al. (1999) Tissue expression and immunolocalization of tumor necrosis factor-alpha in postinfarction dysfunctional myocardium.Circulation. 99: 1492-8. 5. El-Kharashi, O.A. et al. (2011) The Potential Pharmacological and Histological Benefits of Carvedilol on the Hippocampal Post- Stroke Seizures in RatsLife Science Journal, 8: 951-60 6. Elgarf, A.S. et al. (2014) Lipopolysaccharide repeated challenge followed by chronic mild stress protocol introduces a combined model of depression in rats: reversibility by imipramine and pentoxifylline.Pharmacol Biochem Behav. 126: 152-62. 7. Aharon-Hananel G et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.Diabetes. 64 (5): 1780-5.
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