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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Anti-Human C1q Antibody (FITC)   

Anti-Human C1q Antibody

Sheep Anti-Human Polyclonal Antibody

  • FC - Anti-Human C1q Antibody  ABD11938
    Published customer image:Sheep anti Human C1q antibody used to evaluate C1q expression by flow cytometry. Image caption:Effects of SAP on C1q Binding to S. pneumoniae Measured Using Flow Cytometry. (A–C) Proportion of ST2 (A), ST4 (B), and ST23F (C) S. pneumoniae bacteria positive for C1q after incubation in 90 μg/ml human C1q with or without addition of 10 or 50 μg/ml hSAP. (D) Example of flow cytometry histograms of C1q binding to ST4 S. pneumoniae after incubation in 90 µg/ml human C1q with or without addition of 50 μg/ml hSAP. (E) Proportion of S. pneumoniae positive for C1q after incubation in human serum with (black shading) or without (grey shading) addition of 50 μg/ml hSAP, using C1q-depleted serum (white shading) as a negative control. (F) Example of flow cytometry histograms of C1q binding to ST2 S. pneumoniae after incubation in human serum with or without addition of 50 μg/ml hSAP. For panels (A–C) and (E), error bars represent SDs, and p-values are calculated using 2-tailed t tests.From: Yuste J, Botto M, Bottoms SE, Brown JS Serum Amyloid P Aids Complement-Mediated Immunity to Streptococcus pneumoniae.PLoS Pathog 3: e120.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02745
Other Accession P02746, P02747
Reactivity Human
Host Sheep
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 26017 Da
Additional Information
Purification Antisera to human C1q were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Immunogen Human C1q, purified from plasma.
Shelf Life 18 months from the date of despatch.
Gene ID 712
Other Names Complement C1q subcomponent subunit A, C1QA
Target/Specificity Sheep anti-Human C1q antibody recognizes human C1q, a sub-component of the C1 complement component.
Preservative & Stabilisers 0.09% Sodium Azide; 0.1% EACA; 0.01% Benzamidine; 1mM EDTA0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human C1q Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Hwang, H.Y. et al. (2008) Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment.
Mol Immunol. 45: 2570-80. 2. Cragg, M.S. and Glennie, M.J. (2004) Antibody specificity controls in vivo effector mechanisms of anti-CD20 reagents.
Blood. 103: 2738-43. 3. Sárvári, M. et al. (2003) Inhibition of C1q-beta-amyloid binding protects hippocampal cells against complement mediated toxicity.
J Neuroimmunol. 137: 12-8. 4. Lewis, M.J. et al. (2008) The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies.
Mol Immunol. 45: 818-27. 5. Yuste, J. et al. (2007) Serum amyloid P aids complement-mediated immunity to Streptococcus pneumoniae.
PLoS Pathog. 3: 1208-19. 6. Tang, Z. et al. (2013) A human single-domain antibody elicits potent antitumor activity by targeting an epitope in mesothelin close to the cancer cell surface.
Mol Cancer Ther. 12: 416-26. 7. John, M. et al. (2000) Nephrotic syndrome in a patient with IgA deficiency-associated mesangioproliferative glomerulonephritis.
Pathology. 32 (1): 56-8.

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Cat# ABD11938
(40 western blots)
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