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Anti-Human von Willebrand Factor Antibody

Sheep Anti-Human Polyclonal Antibody

     
  • IF - Anti-Human von Willebrand Factor Antibody  ABD11939
    Published customer image:Sheep anti Human von Willebrand Factor antibody used for the evaluation of von Willebrand factor expression in isolated aortic cell preparations by immunofluorescence.Image caption:Characterization of the isolated cells.(A) Representative phase contrast micrographs show the cell population collected at each of the four consecutive rounds of digestion (well 1 to 4) on the intimal (I) and adventitial (A) side of the aorta. In both cases, three main phenotypes were observed: small polygonal cells forming areas of cobblestone appearance, giant mono- or multinucleated cells and elongated cells. All three phenotypes were also detected at P1. Immunofluorescence analysis performed on each P0 population identified ECs by positive vWf staining (insert). The small polygonal mononucleated cells and the giant mono- or polynucleated morphologies, stained both for vWf. Rare elongated cells could also be vWf positive, their unusual shape likely being due to surrounding peer pressure. Magnification of the phase contrast pictures and immunofluorescence images is 10x and 63x, respectively. (B) Immunofluorescent micrographs showing the diversity of vWf presentation. vWf staining performed on adventitial aortic endothelial cells shows diverse and typical vWF staining patterns such as that of elongated WPBs throughout the cell body (d and e), residual vWf granules after degranulation of WPB or seen in cross section (a), reticular vWf around the nucleus corresponding to vWf re-synthesis after degranulation (b and c) or extracellular string of vWf (e). (C) Immunofluorescent micrographs of isolated cells double-stained for vWf together with either CD31 or VE-cadherin validates vWf staining. Small polygonal mono-nucleated cells and most of the giant mono- or polynucleated vWf positive cells also stained for CD31 or VE-cadherin at P0 and P2 and in IEC and AEC.From: Leclercq A, Veillat V, Loriot S, Spuul P, Madonna F, Roques X, et al. A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.PLoS ONE 10: e0143144.
  • IF - Anti-Human von Willebrand Factor Antibody  ABD11939
    Published customer image:Sheep anti Human von Willebrand Factor antibody used for the evaluation of von Willebrand factor expression in isolated proliferating aortic cell preparations by immunofluorescence.Image caption:Analysis of proliferating cells: selection of EC-enriched fractions and validation of procedure.(A) The left-hand morphologic diagram (M) shows the fraction used for western blot analysis in (B) . The high percentage of ECs was confirmed in the corresponding vWf immunofluorescent staining , performed at P2 (200 cells were scored). (B) P2 ECs isolated with the recommended procedure from three patients: 8I, 1I and 10I and A, were lysed and analyzed for three EC and two mesenchymal markers by SDS PAGE. (C) Representative phase contrast images of the cells from the fractions selected for patient 10, stimulated or not by TGFbeta, before cell lysis and western blot analysis, are shown. The biochemical studies performed at P2 cross-validate the morphologic (performed at P0 and P1 stage by phase contrast imaging) and immunophenotypic (performed at P0 and P2 stage by immunofluorescent staining) characterization of the isolated cells.From: Leclercq A, Veillat V, Loriot S, Spuul P, Madonna F, Roques X, et al. A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.PLoS ONE 10: e0143144.
  • IF - Anti-Human von Willebrand Factor Antibody  ABD11939
    Published customer image:Sheep anti Human von Willebrand Factor antibody, used for the identification of von Willebrand expressing endothlial cells in the spinal cord of dark agouti rats with experimental acute encephalomyelitis by immunofluorescence.Image caption:SLPI expression within the spinal cord is associated with ED1-expressing macrophages or activated microglia in the acute phase (score 3.5, A) and with GFAP-positive astrocytes (B) and NeuN expressing neuronal cells (C) especially during the relapsing phase (score 3.5); there was no association of SLPI with von-Willebrand-factor positive endothelial cells (D). Magnification: 100x.From: Mueller AM, Pedr&eacute X, Stempfl T, Kleiter I, Couillard-Despres S, Aigner L, Giegerich G, Steinbrecher A. Novel role for SLPI in MOG-induced EAE revealed by spinal cord expression analysis.J Neuroinflammation. 2008 May 26;5:20.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, IF, FC, E, ID
Primary Accession P04275
Reactivity Human
Host Sheep
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 309265 Da
Additional Information
Other Species Rat
Purification Purified IgG was prepared from serum by ion exchange chromatography
Immunogen Purified human von Willebrand factor prepared from citrated human plasma
Shelf Life 18 months from the date of despatch.
Gene ID 7450
Other Names von Willebrand factor, vWF, von Willebrand antigen 2, von Willebrand antigen II, VWF, F8VWF
Target/Specificity Sheep anti-Human von Willebrand Factor antibody recognizes Human von Willebrand factor, a glycoprotein synthesized in endothelial cells and megakaryocytes and circulating in the blood as a noncovalent complex by association with factor VIII.
Preservative & Stabilisers 0.09% Sodium Azide; 0.1% EACA; 0.01% Benzamidine; 1mM EDTA0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human von Willebrand Factor Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Blades, M.C. et al. (2002) Stromal cell-derived factor 1 (CXCL12) induces human cell migration into human lymph nodes transplanted into SCID mice.
J Immunol. 168 (9): 4308-17. 2. Johnson, L.A. & Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.
Int Immunol. 22 (10): 839-49. 3. Blagoveshchenskaya, A.D. et al. (2002) Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor.
Mol Biol Cell. 13:1582-93. 4. Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.
Blood. 116: 2183-91. 5. Babich, V. et al. (2009) Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body.
J Biol Chem. 284: 12459-68. 6. Butthep, P. et al. (2004) Endothelial injury and altered hemodynamics in thalassemia.
Clin Hemorheol Microcirc. 31: 287-93. 7. Chowdhury, F. et al. (2010) Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability.
Exp Lung Res. 36: 1-11. 8. Johnson, L.A. and Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.
Int Immunol. 22: 839-49. 9. Kiskin, N.I. et al. (2010) Protein mobilities and P-selectin storage in Weibel-Palade bodies.
J Cell Sci. 123: 2964-75. 10. Kriehuber, E. et al. (2001) Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.
J Exp Med. 194: 797-808. 11. Mueller, A.M. et al. (2008) Novel role for SLPI in MOG-induced EAE revealed by spinal cord expression analysis.
J Neuroinflammation. 5: 20. 12. Zhu, B. et al. (2005) Cyclic GMP-specific phosphodiesterase 5 regulates growth and apoptosis in pulmonary endothelial cells.
Am J Physiol Lung Cell Mol Physiol. 289: L196-206. 13. Johnson, L.A. & Jackson, D.G. (2013) The chemokine CX3CL1 promotes trafficking of dendritic cells through inflamed lymphatics.
J Cell Sci. 126 (Pt 22): 5259-70. 14. Leclercq, A. et al. (2015) A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.
PLoS One. 10 (11): e0143144. 15. Cheung, K. et al. (2015) CD31 signals confer immune privilege to the vascular endothelium.
Proc Natl Acad Sci U S A. 112 (43): E5815-24.

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