|Application ||IHC-F, IF, FC, E, ID|
|Calculated MW||309265 Da|
|Purification||Purified IgG was prepared from serum by ion exchange chromatography|
|Immunogen||Purified human von Willebrand factor prepared from citrated human plasma|
|Shelf Life||18 months from the date of despatch.|
|Other Names||von Willebrand factor, vWF, von Willebrand antigen 2, von Willebrand antigen II, VWF, F8VWF|
|Target/Specificity||Sheep anti-Human von Willebrand Factor antibody recognizes Human von Willebrand factor, a glycoprotein synthesized in endothelial cells and megakaryocytes and circulating in the blood as a noncovalent complex by association with factor VIII.|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.1% EACA; 0.01% Benzamidine; 1mM EDTA0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human von Willebrand Factor Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Blades, M.C. et al. (2002) Stromal cell-derived factor 1 (CXCL12) induces human cell migration into human lymph nodes transplanted into SCID mice.J Immunol. 168 (9): 4308-17. 2. Johnson, L.A. & Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.Int Immunol. 22 (10): 839-49. 3. Blagoveshchenskaya, A.D. et al. (2002) Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor.Mol Biol Cell. 13:1582-93. 4. Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.Blood. 116: 2183-91. 5. Babich, V. et al. (2009) Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body.J Biol Chem. 284: 12459-68. 6. Butthep, P. et al. (2004) Endothelial injury and altered hemodynamics in thalassemia.Clin Hemorheol Microcirc. 31: 287-93. 7. Chowdhury, F. et al. (2010) Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability.Exp Lung Res. 36: 1-11. 8. Johnson, L.A. and Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.Int Immunol. 22: 839-49. 9. Kiskin, N.I. et al. (2010) Protein mobilities and P-selectin storage in Weibel-Palade bodies.J Cell Sci. 123: 2964-75. 10. Kriehuber, E. et al. (2001) Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.J Exp Med. 194: 797-808. 11. Mueller, A.M. et al. (2008) Novel role for SLPI in MOG-induced EAE revealed by spinal cord expression analysis.J Neuroinflammation. 5: 20. 12. Zhu, B. et al. (2005) Cyclic GMP-specific phosphodiesterase 5 regulates growth and apoptosis in pulmonary endothelial cells.Am J Physiol Lung Cell Mol Physiol. 289: L196-206. 13. Johnson, L.A. & Jackson, D.G. (2013) The chemokine CX3CL1 promotes trafficking of dendritic cells through inflamed lymphatics.J Cell Sci. 126 (Pt 22): 5259-70. 14. Leclercq, A. et al. (2015) A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.PLoS One. 10 (11): e0143144. 15. Cheung, K. et al. (2015) CD31 signals confer immune privilege to the vascular endothelium.Proc Natl Acad Sci U S A. 112 (43): E5815-24.
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