|Application ||WB, E|
|Purification||Antisera to peptide DYKDDDDK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.|
|Shelf Life||18 months from the date of despatch.|
|Target/Specificity||Rabbit anti-DYKDDDDK Tag antibody recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.Rabbit anti-DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.In Western blotting of bacterial extracts Rabbit anti-DYKDDDDK Tag antibody has been shown not to cross-react with any endogenous proteins.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-DYKDDDDK Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Hopp, T.P. et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6: 1204-1210.
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