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>   home   >   Products   >   Primary Antibodies   >   Anti-DYKDDDDK Tag Antibody (HRP)   

Anti-DYKDDDDK Tag Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Additional Information
Purification Antisera to peptide DYKDDDDK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Shelf Life 18 months from the date of despatch.
Target/Specificity Rabbit anti-DYKDDDDK Tag antibody recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.Rabbit anti-DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.In Western blotting of bacterial extracts Rabbit anti-DYKDDDDK Tag antibody has been shown not to cross-react with any endogenous proteins.
Preservative & Stabilisers 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-DYKDDDDK Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Hopp, T.P. et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification.
Bio/Technology 6: 1204-1210.

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Cat# ABD11947
(40 western blots)
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