|Application ||WB, IHC-P, E|
|Calculated MW||35478 Da|
|Purification||Antisera to human CD254 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Recombinant human CD254.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254, Tumor necrosis factor ligand superfamily member 11, membrane form, Tumor necrosis factor ligand superfamily member 11, soluble form, TNFSF11, OPGL, RANKL, TRANCE|
|Target/Specificity||Rabbit anti-Human CD254 antibody recognizes the soluble form of human CD254, otherwise known as sRANKL, a ~20 kDa glycoprotein and member of the Tumour Necrosis Factor (TNF) superfamily, generated by proteolytic processing of the full-length CD254 membrane protein.CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors, acting as a ligand for both receptor activator of NF-KBeta (RANK) and the decoy receptor osteoprotegerin (OPG), which act as antagonists. Binding of CD254 to RANK is essential for the activation of osteoclastogenesis, stimulated by factors such as TNF-alpha, IL-1beta, IL-6 and IL-17, whereas binding to OPG neutralizes CD254, thereby inhibiting osteoclastogenesis.CD254 augments the dendritic cell (DC) stimulation of naïve T cell proliferation and may be involved in the regulation of DC-T cell interactions. CD254 is also involved in apoptosis, through activation of the antiapoptotic kinase AKT/PKB and the mTOR/S6K signalling pathway.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.|
|Precautions||Anti-Human CD254 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Boyle, W.J. et al. (2003) Osteoclast differentiation and activation.Nature. 423 (6937): 337-42.
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