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Anti-Human RANTES Antibody (Biotin)

Rabbit Anti-Human Polyclonal Antibody

  • IHC - Anti-Human RANTES Antibody (Biotin) ABD11971
    Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human RANTES following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
  • IHC-P - Anti-Human RANTES Antibody (Biotin) ABD11971
    Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human RANTES following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13501
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 9990 Da
Additional Information
Purification Antisera to human RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
Immunogen Recombinant Human RANTES
Shelf Life 12 months from date of reconstitution.
Gene ID 6352
Other Names C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, TCP228, T-cell-specific protein RANTES, RANTES(3-68), RANTES(4-68), CCL5, D17S136E, SCYA5
Target/Specificity Rabbit anti-Human RANTES antibody recognizes human RANTES (regulated upon activation, normal T cell expressed and secreted), otherwise known as CCL5, a 68 amino acid CC- chemokine involved in T cell activation and cell recruitment, expressed by cytotoxic (CD8+) T cells (CTLs), macrophages, keratinocytes and endothelial cells.RANTES acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5. Both CCR3 and CCR5 are known co-receptors for the entry of HIV viruses into target cells and the binding of RANTES to CCR3 on CTLs has been shown to enhance HIV-1-specific cytotoxicity, whilst binding to CCR5 inhibits membrane fusion and hence HIV entry.
Preservative & Stabilisers Preservative Free
Storage Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.
PrecautionsAnti-Human RANTES Antibody (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Appay, V. et al. (2000) RANTES activates antigen-specific cytotoxic T lymphocytes in a mitogen-like manner through cell surface aggregation.
Int. Immunol. 12: 1173-1182. 2. Dragic, T. et al. (1996) HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5.
Nature. 381: 667-673.

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Cat# ABD11971
(40 western blots)
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