|Application ||WB, E|
|Calculated MW||9990 Da|
|Purification||Antisera to human RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.|
|Immunogen||Recombinant Human RANTES|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, TCP228, T-cell-specific protein RANTES, RANTES(3-68), RANTES(4-68), CCL5, D17S136E, SCYA5|
|Target/Specificity||Rabbit anti-Human RANTES antibody recognizes human RANTES (regulated upon activation, normal T cell expressed and secreted), otherwise known as CCL5, a 68 amino acid CC- chemokine involved in T cell activation and cell recruitment, expressed by cytotoxic (CD8+) T cells (CTLs), macrophages, keratinocytes and endothelial cells.RANTES acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5. Both CCR3 and CCR5 are known co-receptors for the entry of HIV viruses into target cells and the binding of RANTES to CCR3 on CTLs has been shown to enhance HIV-1-specific cytotoxicity, whilst binding to CCR5 inhibits membrane fusion and hence HIV entry.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.|
|Precautions||Anti-Human RANTES Antibody (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Appay, V. et al. (2000) RANTES activates antigen-specific cytotoxic T lymphocytes in a mitogen-like manner through cell surface aggregation. Int. Immunol. 12: 1173-1182. 2. Dragic, T. et al. (1996) HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5. Nature. 381: 667-673.
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