|Application ||WB, E|
|Calculated MW||25567 Da|
|Purification||Antisera to bovine TNF alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.|
|Immunogen||Recombinant bovine TNF alpha.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2|
|Target/Specificity||Rabbit anti-Bovine TNF alpha antibody recognizes bovine tumour necrosis factor alpha (TNF alpha).TNF alpha is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells. TNF alpha has a key regulatory role in the bovine immune response, where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine TNF alpha Antibody (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.J Dairy Sci. 93: 2458-70. 2. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. colimastitis.Funct Integr Genomics. 10: 21-38. 3. Cigliano, L. et al. (2016) Evaluation of serum markers of blood redox homeostasis and inflammation in PCB naturally contaminated heifers undergoing decontaminationScience of The Total Environment. 542: 653-64.
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