|Application ||WB, IHC-P, IF, E|
|Calculated MW||44702 Da|
|Purification||Antisera to human BMP-2 were raised by repeated immunisations of rabbits with highly purified antigen.|
|Immunogen||Recombinant human BMP-2|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Bone morphogenetic protein 2, BMP-2, Bone morphogenetic protein 2A, BMP-2A, BMP2, BMP2A|
|Target/Specificity||Rabbit anti-Human BMP-2 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 2 (BMP-2/BMP-2a), full-length 396 amino acids. BMP-2 is a member of the Transforming Growth Factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.The realization that BMP-2 was involved in the stimulation of bone formation emerged from research in spinal surgery, following the discovery that the healing of bones was directed by proteins contained within the bone matrix itself.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.|
|Precautions||Anti-Human BMP-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Bessa, P.C. et al. (2008) Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli.Biotechnol Lett. 30: 15-21.1. Kimura, N. et al. (2000) BMP2-induced apoptosis is mediated by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6.J. Biol. Chem. 275: 17647-17652.2. Kirsch, T. et al. (2000) BMP-2 antagonists emerge from alterations in the low-affinity binding epitope for receptor BMPR-II.EMBO J. 19: 3314-3324.3. Keller, S. et al. (2004) Molecular recognition of BMP-2 and BMP receptor IA.Nature Structural and Molecular Biology. 11: 481-488.4. Balemans, W. and Van Hul, W. (2002) Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators.Dev. Biol. 250: 231-250.5. Sebald, W. et al. (2004) Molecular recognition in bone morphogenetic protein (BMP)/receptor interaction. Biol. Chem. 385: 697-710.
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