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>   home   >   Products   >   Primary Antibodies   >   Anti-Rat OX-62 Antibody, clone OX-62 (RPE)   

Anti-Rat OX-62 Antibody, clone OX-62 (Biotin)

Mouse Anti-Rat Monoclonal Antibody

  • FC - Anti-Rat OX-62 Antibody, clone OX-62 (Biotin) ABD12004
    Published customer image:IL-4 DC and IL-10 DC exhibit no obvious differences in their phenotype. IL-4 DC and IL-10 DC and mature splenic DC (sDC) coexpressed Ox62 and CD68, whereas macrophages generated in the presence of M-CSF (5 µg/ml) were only positive for CD68. Broken lines indicate background staining obtained using an irrelevant isotype control. The first number represents the percentage of cells staining positive for the indicated marker and the second number represents the mean fluorescence intensity. The results shown are representative for 4 independent flow cytometric analyses.From:BMC Res Notes. 2009 Jan 23;2:12.
  • ICC - Anti-Rat OX-62 Antibody, clone OX-62 (Biotin) ABD12004
    Published customer image:Morphology and immunostaining of IL-4 DC and IL-10 DC. Rat BMDC were isolated from cell clusters on day 6 of culture (A). Cells prepared on cytospin slides stained positive for monoclonal antibodies Ox62 (rat DC marker) (B), Ox6 (MHC class II) (C), and CD68 (D). Shown are representative IL-4 DC results, which are similar to those for IL-10 DC. Magnification: ×200 (A, C, D) and ×400 (B).From: Tiurbe G, Matuschek A, Kämmerer U, Schneider M, Thiede A, Ulrichs K, Otto C. Inhibitory effects of rat bone marrow-derived dendritic cells on naïve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10. BMC Res Notes. 2009 Jan 23;2:12.
  • FC - Anti-Rat OX-62 Antibody, clone OX-62 (Biotin) ABD12004
    Rat Splenocytes stained with Mouse anti Rat OX-62:RPE
  • IHC - Anti-Rat OX-62 Antibody, clone OX-62 (Biotin) ABD12004
    Published customer image:Analysis of innate immune cells into a corneal graft. Ox-62+ DC and CD163+ macrophages were stained at the time points of corneal allograft rejection and calculated within the graft. Additionally CD161+ cells were counted within rejected corneal allografts to finally prove the efficacy of the depletion protocol in the peripheral tissue. Representative histological staining is shown for Ox-62 (A), CD163 (C), and CD161 (E) in NK deficient and control animals. B: No statistical difference was observed for infiltrating Ox-62+ DC. D: CD163+ macrophages infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names OX-62
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Density gradient enriched PVG rat thoracic duct dendritic cells.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Rat OX-62 antibody, clone OX-62 recognizes the OX-62 antigen, shown to be rat alpha E2 integrin which is expressed by dendritic cells of dendritic epidermal T cells, intraepithelial lymphocytes in the small intestine and by cells of dendritic morphology in lymphoid organs, at sites where gamma delta T cells are present.
Preservative & Stabilisers 0.09% Sodium Azide; 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Rat OX-62 Antibody, clone OX-62 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Brenan, M. and Puklavec, M. (1992) The MRC OX-62 Antigen: A useful marker in the purification of rat veiled cells with the biochemical properties of an integrin.
J. Exp. Med. 175: 1457-1465. 2. Brenan, M. and Rees, D.J. (1997) Sequence analysis of rat integrin alpha E1 and alpha E2 subunits: tissue expression reveals plenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph.
Eur. J. Immunol. 27: 3070-3079. 3. Kostulas, N. et al. (2002) Dendritic cells are present in ischemic brain after permanent middle cerebral artery occlusion in the rat.
Stroke. 33: 1129-1134. 4. Rival, C. et al. (2006) Identification of a dendritic cell population in normal testis and in chronically inflamed testis of rats with autoimmune orchitis.
Cell Tissue Res. 324: 311-318 5. Tsuchiya, T. et al. (2002) Dendritic cell involvement in pulmonary granuloma formation elicited by bacillus calmette-guerin in rats.
Am. J. Respir. Crit. Care Med. 165: 1640-1646. 6. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
J. Neuroimmunol. 209: 16-25. 7. Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
Mol. Vis. 16: 1928-1935. 8. Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.
Virology. 388: 78-90. 9. Zhou. Y.J. et al. (2010) The role of the lactadherin in promoting intestinal DCs development in vivo and vitro.
Clin Dev Immunol.2010: 357541. 10. Aiello, S. et al. (2000) Thymic Dendritic Cells Express Inducible Nitric Oxide Synthase and Generate Nitric Oxide in Response to Self- and Alloantigens
J Immunol. 164: 4649-58. 11. Camelo, S. et al. (2003) Local retention of soluble antigen by potential antigen-presenting cells in the anterior segment of the eye.
Invest Ophthalmol Vis Sci. 44: 5212-9. 12. Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
J Immunol. 172: 5388-95. 13. Henry, F. et al. (1999) Antigen-presenting cells that phagocytose apoptotic tumor-derived cells are potent tumor vaccines.
Cancer Res. 59: 3329-32. 14. Liu, L. et al. (1998) Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression.
J Immunol. 161: 1146-55. 15. O'Sullivan, N.L. et al. (2001) Lymphocyte lineages at mucosal effector sites: rat salivary glands.
J Immunol. 166: 5522-9. 16. Penttila, I.A. et al. (2003) Maternal milk regulation of cell infiltration and interleukin 18 in the intestine of suckling rat pups.
Gut. 52: 1579-86. 17. Stephens, L.A. et al. (2004) Phenotypic characterization of regulatory CD4+CD25+ T cells in rats.
Int Immunol. 16: 365-75. 18. Sugawara, I. et al. (2004) Pathological and immunological profiles of rat tuberculosis.
Int J Exp Pathol. 85: 125-34. 19. Tiurbe, G. et al. (2009) Inhibitory effects of rat bone marrow-derived dendritic cells on naïve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10.
BMC Res Notes. 2: 12. 20. Tsuchiya, T. et al. (2002) Dendritic cell involvement in pulmonary granuloma formation elicited by bacillus calmette-guérin in rats.
Am J Respir Crit Care Med. 165: 1640-6.

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Cat# ABD12004
(40 western blots)
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