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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Mouse CD45 Antibody, clone YW62.3 (RPE)   

Anti-Mouse CD45 Antibody, clone YW62.3 (FITC)

Rat Anti-Mouse Monoclonal Antibody

  • IF - Anti-Mouse CD45 Antibody, clone YW62.3 (FITC) ABD12005
    Published customer image:Rat anti Mouse CD31 antibody, clone YW62.3 used for the detection of microglia in mouse brain by immunofluorescence on cryostat sections.Image caption:Immunofluorescent labeling of microglial activation and phospho-tau in rTg4510 mice treated with LPS. Panels A-C shows immunofluorescent staining of arginase-1 positive microglia (green; A, C) and phospho-tau ser396 (red; B, C) in the anterior cortex. Panels D-F shows YM1 positive microglia (green; D, F) and AT8 (phospho-tauSer202/Thr204) (red; E, F) in the anterior cortex. Panels G-L shows increased CD45 positive microglia (Green; G, I, J, L), phospho-tauSer199/202 (red; H, I), and full-length tau (red; K, L) in the hippocampus CA region. Activated microglia failed to co-label with phospho-tau markers. Images were taken at 20x objective. Scale bar represents 20 µm.From: Lee DC, Rizer J, Selenica ML, Reid P, Kraft C, Johnson A, Blair L, Gordon MN, Dickey CA, Morgan D. LPS- induced inflammation exacerbates phospho-tau pathology in rTg4510 mice. J Neuroinflammation. 2010 Sep 16;7:56.
  • FC - Anti-Mouse CD45 Antibody, clone YW62.3 (FITC) ABD12005
    Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD45 (Ly-5):Alexa Fluor® 647
  • IHC - Anti-Mouse CD45 Antibody, clone YW62.3 (FITC) ABD12005
    Published customer image:Rat anti Mouse CD31 antibody, clone YW62.3 used for the detection of microglia in mouse brain by immunohistochemistry on cryostat sections.Image caption:LPS-Induced CD45 Activation in rTg4510 and nontransgenic mice. The microglia marker CD45 was examined by immunohistochemical staining in anterior cortex, hippocampus , and entorhinal cortex after LPS administration. rTg4510 mice or nontransgenic littermates were injected unilaterally with LPS or vehicle into the anterior cortex and hippocampus, followed by 1 week survival. Representative images from anterior cortex of nontransgenic (A, C) or rTg4510 mice (B, D) from vehicle- (A, B) or LPS-injected (C, D) mice are shown. Images (F-I) were collected from the CA1 stratum radiatum of non-transgenic (F, H) or rTg4510 mice (G, I) after vehicle (F, G) or LPS (H, I) injection. Similar images (K-N) were collected from the entorhinal cortex of nontransgenic (K, M) or rTg4510 mice (L, N) after vehicle (K, L) or LPS (M, N) injection. Quantitation of % area containing positive immunostaing is presented for each brain region in E, J, and O (mean ± S.E.M, n = 6-8). CD45+ staining increased in both rTg4510 and nontransgenic littermates in anterior cortex, CA1, and ECX of mice treated with LPS compared to vehicle-treated mice. Statistical analysis was performed using 2-way ANOVA followed by Fisher's PLSD multiple comparison test. The asterisk indicates *p < 0.05), n = 6 = 8. Scale bar represents 20 µm.From: Lee DC, Rizer J, Selenica ML, Reid P, Kraft C, Johnson A, Blair L, Gordon MN, Dickey CA, Morgan D. LPS- induced inflammation exacerbates phospho-tau pathology in rTg4510 mice. J Neuroinflammation. 2010 Sep 16;7:56.
  • FC - Anti-Mouse CD45 Antibody, clone YW62.3 (FITC) ABD12005
    Staining of mouse splenocytes with Rat anti Mouse CD45: FITC
  • FC - Anti-Mouse CD45 Antibody, clone YW62.3 (FITC) ABD12005
    Staining of mouse spleen with Rat anti Mouse CD45: RPE
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06800
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names YW62.3
Calculated MW 144604 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Mouse spleen cells.
Shelf Life 12 months from date of reconstitution.
Other Names Receptor-type tyrosine-protein phosphatase C,, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5
Target/Specificity Rat anti-Mouse CD45 antibody, clone YW62.3 recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kDa glycoprotein expressed by all leucocytes.CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains (Komuraet al.1975), this was subsequently expanded to include a third allele encoding Ly5.3 (Shenet al.1986). Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1 (Morseet al.1987). Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb(Charbonneauet al.1988;Zebedeeet al.1991).A number of different isoforms isoforms of CD45 are expressed on murine leucocytes depending on the pattern of altenative splicing of 3 exons termed A, B and C encoding regions of ~ 50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. (Birkelandet al.1989).Rat anti-mouse CD45 antibody, clone YW62.3 is reactive with all isoforms of murine CD45. N.B. Some reactivity with human tissue has been observed.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Mouse CD45 Antibody, clone YW62.3 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Mol. Cell. Probes 1: 297-326. 2. Bindon, C.I. et al. (1988) Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies.
Eur. J. Immunol. 18: 1507-1514. 3. Long, G.G. et al. (2010) Hematopoietic Proliferative Lesions in the Spleen of rasH2 Transgenic Mice Treated with MNU.
Toxicol Pathol. 38: 1026-36. 4. Drake, C. et al. (2011) Brain inflammation is induced by co-morbidities and risk factors for stroke.
Brain Behav Immun. 25: 1113-22. 5. Chan, D.A. et al (2009) Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment.
Cancer Cell. 15: 527-38. 6. Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice.
J Neurosci. 30: 9651-8. 7. Lee, D.C. et al. (2010) LPS- induced inflammation exacerbates phospho-tau pathology in rTg4510 mice.
J Neuroinflammation. 7: 56. 8. Wang, S. et al. (2008) Drak2 contributes to West Nile virus entry into the brain and lethal encephalitis.
J Immunol. 181: 2084-91. 9. Paz, H. et al. (2010) The homeobox gene Hhex regulates the earliest stages of definitive hematopoiesis.
Blood. 116: 1254-62. 10. Reed-Geaghan, E.G. et al. (2010) Deletion of CD14 attenuates Alzheimer's disease pathology by influencing the brain's inflammatory milieu.
J Neurosci. 30: 15369-73. 11. Yang, R. et al. (2010) Successful treatment of experimental glomerulonephritis with IdeS and EndoS, IgG-degrading streptococcal enzymes.
Nephrol Dial Transplant. 25: 2479-86. 12. Yang, J. et al. (2010) Evaluation of bone marrow- and brain-derived neural stem cells in therapy of central nervous system autoimmunity.
Am J Pathol. 177: 1989-2001. 13. Yoshizaki, A. et al. (2010) Cell adhesion molecules regulate fibrotic process via Th1/Th2/Th17 cell balance in a bleomycin-induced scleroderma model.
J Immunol. 185: 2502-15. 14. Abramowski, D. et al. (2012) Transgenic Expression of Intraneuronal Aß42 But Not Aß40 Leads to Cellular Aß Lesions, Degeneration, and Functional Impairment without Typical Alzheimer's Disease Pathology.
J Neurosci. 32: 1273-83. 15. Dénes, A. et al. (2010) Chronic systemic infection exacerbates ischemic brain damage via a CCL5 (regulated on activation, normal T-cell expressed and secreted)-mediated proinflammatory response in mice.
J Neurosci. 30: 10086-95. 16. Kondo, Y. et al. (2007) Osteopetrotic (op/op) mice have reduced microglia, no Abeta deposition, and no changes in dopaminergic neurons.
J Neuroinflammation. 4: 31. 17. Lee, S. et al. (2010) CX3CR1 deficiency alters microglial activation and reduces beta-amyloid deposition in two Alzheimer's disease mouse models.
Am J Pathol. 177: 2549-62. 18. Kim, J. et al. (2012) Anti-apoE immunotherapy inhibits amyloid accumulation in a transgenic mouse model of Aß amyloidosis.
J Exp Med. 209: 2149-56. 19. Yamauchi, S. et al. (2012) Myosin II-dependent exclusion of CD45 from the site of Fcγ receptor activation during phagocytosis.
FEBS Lett. 586: 3229-35. 20. Yazid, S. et al. (2015) Annexin-A1 restricts Th17 cells and attenuates the severity of autoimmune disease.
J Autoimmun. pii: S0896-8411(14)00183-8. 21. Kan, M.J. et al. (2015) Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
J Neurosci. 35 (15): 5969-82. 22. Bachstetter, A.D. et al. (2011) Fractalkine and CX 3 CR1 regulate hippocampal neurogenesis in adult and aged rats.
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J Immunol. 180 (3): 1353-61. 24. Murinello, S. et al. (2014) Fcγ receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration.
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Cat# ABD12005
(40 western blots)
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