|Calculated MW||26036 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||T-cell surface glycoprotein CD8 alpha chain, CD8a, CD8A|
|Target/Specificity||Rat anti-Dog CD8 antibody, clone YCATE55.9 was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop (Cobboldet al.1994). YCATE55.9 reacts with a rat cell line transfected with cDNA for canine CD8α (Gormanet al.1994) and blocks MHC class I dependant T-cell responses in vitroandin vivo.Rat anti-Dog CD8, clone YCATE55.9 has been shown to deplete circulating CD8+ T cells when administered to dogs in vivo. (Watsonet al.1993) Reduced levels of circulating CD8+ T cells has been associated with decreased survival times for dogs with osteosarcoma (Billeret al.2010)|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Dog CD8 Antibody, clone YCATE55.9 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Cobbold, S and Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 43: 137 - 154. 2. Gorman, S. et al. (1994) Isolation and expression of cDNA encoding the canine CD4 and CD8a antigens. Tissue Antigens. 43:184 - 188. 3. Watson, C. J. E. et al. (1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog. Brit. J. Surg. 80: 1389 - 1392. 4. Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.Can J Vet Res. 73: 137-143. 5. Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.J Nutr. 133: 1158-62. 6. Bird, R.C. et al. (2010) An autologous dendritic cell canine mammary tumor hybrid-cell fusion vaccine.Cancer Immunol Immunother. 60: 87-97. 7. Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.Cell Immunol. 263: 88-98. 8. Estrela-Lima, A. et al. (2010) Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates.BMC Cancer. 10: 256. 9. Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.J Leukoc Biol. 84: 1501-10. 10. Kornegay, J.N. et al. (2010) Widespread muscle expression of an AAV9 human mini-dystrophin vector after intravenous injection in neonatal dystrophin-deficient dogs.Mol Ther. 18: 1501-8. 11. Pichavant, C. et al. (2010) Expression of dog microdystrophin in mouse and dog muscles by gene therapy.Mol Ther. 18: 1002-9. 12. Pinheiro, D. et al (2011) Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog.Immunology. 132: 111-22. 13. Reis, A.B. et al. (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.Clin Exp Immunol. 146: 303-11. 14. Figueiredo, M.M. et al. (2014) Expression of Regulatory T Cells in Jejunum, Colon, and Cervical and Mesenteric Lymph Nodes of Dogs Naturally Infected with Leishmania infantum.Infect Immun. 82: 3704-12. 15. Costa-Pereira, C. et al. (2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.BMC Vet Res. 11 (1): 92.
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