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>   home   >   Products   >   Primary Antibodies   >   Anti-Dog CD8 Antibody, clone YCATE55.9 (RPE)   

Anti-Dog CD8 Antibody, clone YCATE55.9 (FITC)

Rat Anti-Dog Monoclonal Antibody

  • FC - Anti-Dog CD8 Antibody, clone YCATE55.9 (FITC) ABD12008
    Staining of canine peripheral blood lymphocytes with Rat anti Canine CD8:FITC
  • FC - Anti-Dog CD8 Antibody, clone YCATE55.9 (FITC) ABD12008
    Staining of canine peripheral blood lymphocytes with Rat anti Dog CD8
  • FC - Anti-Dog CD8 Antibody, clone YCATE55.9 (FITC) ABD12008
    Published customer image:Rat anti Dog CD8 antibody, clone YCATE55.9 used for the identification of CD8 expressing dog lymphocytes by flow cytometry.Image caption:Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes.From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92.
  • FC - Anti-Dog CD8 Antibody, clone YCATE55.9 (FITC) ABD12008
    Published customer image:CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P33706
Reactivity Dog
Host Rat
Clonality Monoclonal
Isotype IgG1
Clone Names YCATE55.9
Calculated MW 26036 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.
Shelf Life 18 months from date of despatch.
Gene ID 403157
Other Names T-cell surface glycoprotein CD8 alpha chain, CD8a, CD8A
Target/Specificity Rat anti-Dog CD8 antibody, clone YCATE55.9 was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop (Cobboldet al.1994). YCATE55.9 reacts with a rat cell line transfected with cDNA for canine CD8α (Gormanet al.1994) and blocks MHC class I dependant T-cell responses in vitroandin vivo.Rat anti-Dog CD8, clone YCATE55.9 has been shown to deplete circulating CD8+ T cells when administered to dogs in vivo. (Watsonet al.1993) Reduced levels of circulating CD8+ T cells has been associated with decreased survival times for dogs with osteosarcoma (Billeret al.2010)
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Dog CD8 Antibody, clone YCATE55.9 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Cobbold, S and Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43: 137 - 154. 2. Gorman, S. et al. (1994) Isolation and expression of cDNA encoding the canine CD4 and CD8a antigens.
Tissue Antigens. 43:184 - 188. 3. Watson, C. J. E. et al. (1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog.
Brit. J. Surg. 80: 1389 - 1392. 4. Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-gamma and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.
Can J Vet Res. 73: 137-143. 5. Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.
J Nutr. 133: 1158-62. 6. Bird, R.C. et al. (2010) An autologous dendritic cell canine mammary tumor hybrid-cell fusion vaccine.
Cancer Immunol Immunother. 60: 87-97. 7. Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.
Cell Immunol. 263: 88-98. 8. Estrela-Lima, A. et al. (2010) Immunophenotypic features of tumor infiltrating lymphocytes from mammary carcinomas in female dogs associated with prognostic factors and survival rates.
BMC Cancer. 10: 256. 9. Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.
J Leukoc Biol. 84: 1501-10. 10. Kornegay, J.N. et al. (2010) Widespread muscle expression of an AAV9 human mini-dystrophin vector after intravenous injection in neonatal dystrophin-deficient dogs.
Mol Ther. 18: 1501-8. 11. Pichavant, C. et al. (2010) Expression of dog microdystrophin in mouse and dog muscles by gene therapy.
Mol Ther. 18: 1002-9. 12. Pinheiro, D. et al (2011) Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog.
Immunology. 132: 111-22. 13. Reis, A.B. et al. (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.
Clin Exp Immunol. 146: 303-11. 14. Figueiredo, M.M. et al. (2014) Expression of Regulatory T Cells in Jejunum, Colon, and Cervical and Mesenteric Lymph Nodes of Dogs Naturally Infected with Leishmania infantum.
Infect Immun. 82: 3704-12. 15. Costa-Pereira, C. et al. (2015) One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
BMC Vet Res. 11 (1): 92.

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Cat# ABD12008
(40 western blots)
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