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>   home   >   Products   >   Primary Antibodies   >   Anti-Dog CD45 Antibody, clone YKIX716.13 (RPE)   

Anti-Dog CD45 Antibody, clone YKIX716.13 (APC)

Rat Anti-Dog Monoclonal Antibody

     
  • FC - Anti-Dog CD45 Antibody, clone YKIX716.13 (APC) ABD12009
    Published customer image:Flow cytometric analyses of optimized platelet samples after density barrier centrifugation. (A) Log forward and side scatter chart of platelet population (gated) with minimal contaminating cells. (B) Fluorescence chart of CD45 and CD61 antibody-labeled samples. Average CD61+: 99.47?±?0.21% (n?=?3). Average CD45+: 0.19?±?0.04% (n?=?3). (C) LeucoCOUNT chart for sample showing residual leukocytes (left gate) and LeucoCOUNT beads (right gate).From: Trichler SA, Bulla SC, Thomason J, Lunsford KV, Bulla C. Ultra-pure platelet isolation from canine whole blood. BMC Vet Res. 2013 Jul 17;9:144.
  • FC - Anti-Dog CD45 Antibody, clone YKIX716.13 (APC) ABD12009
    Staining of canine peripheral blood lymphocytes with Rat anti Canine CD45:FITC
  • FC - Anti-Dog CD45 Antibody, clone YKIX716.13 (APC) ABD12009
    Staining of canine peripheral blood lymphocytes with Rat anti Canine CD45:Alexa Fluor®488
  • FC - Anti-Dog CD45 Antibody, clone YKIX716.13 (APC) ABD12009
    Published customer image:Flow cytometric analyses of optimized platelet samples after density barrier centrifugation. (A) Log forward and side scatter chart of platelet population with minimal contaminating cells. (B) Fluorescence chart of CD45 and CD61 antibody-labeled samples. Average CD61+: 99.47?±?0.21% (n?=?3). Average CD45+: 0.19?±?0.04% (n?=?3). (C) LeucoCOUNT chart for sample showing residual leukocytes (left gate) and LeucoCOUNT beads (right gate).From: Trichler SA, Bulla SC, Thomason J, Lunsford KV, Bulla C. Ultra-pure platelet isolation from canine whole blood. BMC Vet Res. 2013 Jul 17;9:144.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Dog
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names YKIX716.13
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Canine thymocytes.
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti-Dog CD45 antibody, clone YKIX716.13 recognizes canine CD45 also known as leukocyte common antigen lustered as Canine CD45 in the First Canine Leukocyte Antigen Workshop (CLAW). Clone YKIX 716.13: immunoprecipitates an antigen of 180/200kD from Con-A blasts (Cobboldet al.1994). CD45 is expressed on all leukocytes in canine peripheral blood. Rat anti-Dog CD45 antibody, clone YKIX716.13 reacts with CD45 on all outbred mongrels and beagles tested and may be against CD45RB isoform.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Prior to reconstitution store at +4℃.
PrecautionsAnti-Dog CD45 Antibody, clone YKIX716.13 (APC) is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens 43(3): 137-154. 2. Reis, A.B. et al (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.
Clin Exp Immunol.146: 303-11. 3. Stein, V.M. et al. (2008) Immunophenotypical characterization of monocytes in canine distemper virus infection.
Vet Microbiol. 131:237-46. 4. Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
Am J Trop Med Hyg. 70: 618-24. 5. Modiano,J.F. and Helfand,S.C. (2011) Early detection of hemangiosarcoma and angiosarcoma
Patent Application No.11/662529 6. Tominaga, M. et al. (2010) Flow cytometric analysis of peripheral blood and tumor-infiltrating regulatory T cells in dogs with oral malignant melanoma.
J Vet Diagn Invest. 22: 438-41. 7. Zentek, J. et al. (2002) Morphology and immunopathology of the small and large intestine in dogs with nonspecific dietary sensitivity.
J Nutr. 132: 1652S-4S. 8. Hunter, M.J. et al. (2011) Gene therapy of canine leukocyte adhesion deficiency using lentiviral vectors with human CD11b and CD18 promoters driving canine CD18 expression.
Mol Ther. 19: 113-21. 9. Comazzi, S. et al. (2006) Flow cytometric patterns in blood from dogs with non-neoplastic and neoplastic hematologic diseases using double labeling for CD18 and CD45.
Vet Clin Pathol. 35: 47-54. 10. Giantin, M. et al. (2013) Evaluation of tyrosine-kinase receptor c-KIT (c-KIT) mutations, mRNA and protein expression in canine leukemia: might c-KIT represent a therapeutic target?
Vet Immunol Immunopathol. 152: 325-32. 11. Trichler, S.A. et al. (2013) Ultra-pure platelet isolation from canine whole blood.
BMC Vet Res. 9: 144. 12. Aresu, L. et al. (2014) VEGF and MMP-9: biomarkers for canine lymphoma.
Vet Comp Oncol. 12: 29-36. 13. Salinas Tejedor, L. et al. (2015) Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system.
Brain Behav Immun. pii: S0889-1591(15)00233-0.

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