|Other Species||Hooded Seal|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Con A activated canine peripheral blood cells.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Rat anti-Dog MHC Class II Monomorphic antibody, clone YKIX334.2 recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobboldet al.1994).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Rat anti-Dog MHC Class II immunoprecipitates an antigen of ~32/34kDa and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Cobbold, S. and Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the first International Canine Leucocyte Antigen Workshop.Tissue Antigens 43: 137-154. 2. Watson, C.J.E. et al. (1994) Immunosuppression of canine renal allograft recipients by CD4 and CD8 monoclonal antibodies.Tissue Antigens 43: 155-162. 3. Reis, A.B. et al. (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi. Clin Exp Immunol.146: 303-11. 4. Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.Vet Immunol Immunopathol. 141: 64-75. 5. Bonnefont-Rebeix, C. et al. (2007) Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC).Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):134-9. 6. Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.Cell Immunol. 263: 88-98. 7. Mito, K. et al. (2010) IFNγ markedly cooperates with intratumoral dendritic cell vaccine in dog tumor models.Cancer Res. 70: 7093-101. 8. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.Immunology. 105: 499-508. 9. Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.Am J Trop Med Hyg. 70: 618-24. 10. Schütze, N. et al. (2009) Inactivated parapoxvirus ovis activates canine blood phagocytes and T lymphocytes.Vet Microbiol. 137: 260-7. 11. Liu, Y. et al. (2000) Immunosuppressant-free allotransplantation of the tracheaThe antigenicity of tracheal grafts can be reduced by removing the epithelium and mixed glands from the graft by detergent treatment. J Thorac Cardiovasc Surg. 120: 108-14. 12. Larsen, A.K. et al. (2013) Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro.PLoS One. 8: e70186.
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