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>   home   >   Products   >   Primary Antibodies   >   Anti-Dog MHC Class II Monomorphic Antibody, clone YKIX334.2    

Anti-Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 (FITC)

Rat Anti-Dog Monoclonal Antibody

  • FC - Anti-Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 (FITC) ABD12011
    Published customer image: Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs. PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b+CD14-MHCII- cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b+CD14-MHCII- cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b+CD14-MHCII- cells is shown at 63× magnification.From: Goulart MR, Pluhar GE, Ohlfest JR Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7: e33274.
  • FC - Anti-Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 (FITC) ABD12011
    Staining of canine peripheral blood lymphocytes with Rat anti Canine MHC Class II: FITC
  • FC - Anti-Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 (FITC) ABD12011
    Published customer image:CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Dog
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names YKIX334.2
Additional Information
Other Species Hooded Seal
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Con A activated canine peripheral blood cells.
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti-Dog MHC Class II Monomorphic antibody, clone YKIX334.2 recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobboldet al.1994).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Rat anti-Dog MHC Class II immunoprecipitates an antigen of ~32/34kDa and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Dog MHC Class II Monomorphic Antibody, clone YKIX334.2 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Cobbold, S. and Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the first International Canine Leucocyte Antigen Workshop.
Tissue Antigens 43: 137-154. 2. Watson, C.J.E. et al. (1994) Immunosuppression of canine renal allograft recipients by CD4 and CD8 monoclonal antibodies.
Tissue Antigens 43: 155-162. 3. Reis, A.B. et al. (2006) Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi.
Clin Exp Immunol.146: 303-11. 4. Araújo, M.S. et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
Vet Immunol Immunopathol. 141: 64-75. 5. Bonnefont-Rebeix, C. et al. (2007) Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC).
Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):134-9. 6. Bund, D. et al. (2010) Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.
Cell Immunol. 263: 88-98. 7. Mito, K. et al. (2010) IFNγ markedly cooperates with intratumoral dendritic cell vaccine in dog tumor models.
Cancer Res. 70: 7093-101. 8. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
Immunology. 105: 499-508. 9. Sanchez, M.A. et al. (2004) Organ-specific immunity in canine visceral leishmaniasis: analysis of symptomatic and asymptomatic dogs naturally infected with Leishmania chagasi.
Am J Trop Med Hyg. 70: 618-24. 10. Schütze, N. et al. (2009) Inactivated parapoxvirus ovis activates canine blood phagocytes and T lymphocytes.
Vet Microbiol. 137: 260-7. 11. Liu, Y. et al. (2000) Immunosuppressant-free allotransplantation of the tracheaThe antigenicity of tracheal grafts can be reduced by removing the epithelium and mixed glands from the graft by detergent treatment.
J Thorac Cardiovasc Surg. 120: 108-14. 12. Larsen, A.K. et al. (2013) Entry and elimination of marine mammal Brucella spp. by hooded seal (Cystophora cristata) alveolar macrophages in vitro.
PLoS One. 8: e70186.

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Cat# ABD12011
(40 western blots)
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