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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD59 Antibody, clone MEM-43    

Anti-Human CD59 Antibody, clone MEM-43

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD59 Antibody, clone MEM-43  ABD12013
    Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Biotin
  • FC - Anti-Human CD59 Antibody, clone MEM-43  ABD12013
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD59
  • FC - Anti-Human CD59 Antibody, clone MEM-43  ABD12013
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD59
  • FC - Anti-Human CD59 Antibody, clone MEM-43  ABD12013
    Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Alexa Fluor® 647
  • WB - Anti-Human CD59 Antibody, clone MEM-43  ABD12013
    Published customer image:Mouse anti Human CD59 antibody, clone MEM-43 used for the detection of CD59 in virus infected cell lysates by western blotting.Image caption:The effect of protease treatment on influenza virion associated host proteins. Purified influenza A/WSN/33 virus was either mock treated or subjected to overnight digestion with subtilisin followed by concentration through a sucrose cushion. 10 ug of mock infected cell lysate (lane 1) or influenza infected cell lysate (lane 2) and 2 ug of untreated influenza virions (lane 3) or protease treated influenza virions (lane 4) were then analyzed by western blot with antibodies against the indicated proteins. Numbers to the right are molecular weight markers.From: Shaw ML, Stone KL, Colangelo CM, Gulcicek EE, Palese P Cellular Proteins in Influenza Virus Particles. PLoS Pathog 4(6): e1000085.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13987
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names MEM-43
Calculated MW 14177 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Thymocytes and T lymphocytes.
Shelf Life 18 months from date of despatch.
Gene ID 966
Other Names CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-inhibitory protein, MAC-IP, MEM43 antigen, Membrane attack complex inhibition factor, MACIF, Membrane inhibitor of reactive lysis, MIRL, Protectin, CD59, CD59, MIC11, MIN1, MIN2, MIN3, MSK21
Target/Specificity Mouse anti-Human CD59 antibody, clone MEM-43 recognizes CD59, a glycosyl-phosphatidylinositol (GPI) anchored membrane protein also known as membrane attack complex inhibition factor. CD59 blocks the formation of the complement membrane attack complex (MAC) by binding of C8a and C9. CD59 is found on all types of leucocytes including platelets and is also expressed on many non-haematopoietic cells. The epitope recognized by Mouse anti-Human CD59 antibody, clone MEM-43 is lost after reduction therefore, non-reducing conditions are required for western blotting techniques.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD59 Antibody, clone MEM-43 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Stefanova, I. et al. (1989) in Leucocyte Typing IV: White cell differentiation antigens.
Ed. Knapp, W. et al. Oxford University Press pp 678-697. 2. Stefanova, I. et al. (1989) Characterisation of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphotidylinositol.
Mol. Immunol. 26: 153 - 161. 3. Tandon, N. et al. (1994) . Expression and function of multiple regulators of complement activation in autoimmune thyroid disease.
Immunology 81: 643-647. 4. Horejsi, V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
Folia. Biol. (Prague) 34: 23-24. 5. Stefanova, I. et al. (1991) Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules.
J. Immunol. 147: 1587 - 1592. 6. Shaw, M.L. et al. (2008) Cellular proteins in influenza virus particles.
PLoS Pathog. 4: e1000085. 7. Sadallah, S. et al. (2011) Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells.
J Immunol. 186: 6543-52. 8. Jolly, C, and Sattentau. Q.J. (2005) Human Immunodeficiency Virus Type 1 Virological Synapse Formation in T Cells Requires Lipid Raft Integrity
J Virol. 79: 12088-94. 9. Shamri, R. et al. (2002) Chemokine stimulation of lymphocyte alpha 4 integrin avidity but not of leukocyte function-associated antigen-1 avidity to endothelial ligands under shear flow requires cholesterol membrane rafts.
J Biol Chem. 277: 40027-35. 10. Bonnon, C. et al. (2010) Selective export of human GPI-anchored proteins from the endoplasmic reticulum.
J Cell Sci. 123: 1705-15. 11. Zhang, J. et al. (2002) Early complement activation and decreased levels of glycosylphosphatidylinositol-anchored complement inhibitors in human and experimental diabetic retinopathy.
Diabetes. 51: 3499-504. 12. Ellison, B.S. et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells.
Cell Div. 2007 2: 20. 13. Cowan, P.J. et al. (1998) High-level endothelial expression of human CD59 prolongs heart function in an ex vivo model of xenograft rejection.
Transplantation. 65: 826-31. 14. Vanderplasschen, A. et al. (1997) Extracellular enveloped vaccinia virus is resistant to complement because of incorporation of host complement control proteins into its envelope.
Proc Natl Acad Sci U S A. 95: 7544-9. 15. Takemoto, M. et al. (2007) Human herpesvirus 7 infection increases the expression levels of CD46 and CD59 in target cells.
J Gen Virol. 88: 1415-22. 16. Chong, Y.H. and Lee, M.J. (2000) Expression of complement inhibitor protein CD59 in human neuronal and glial cell lines treated with HIV-1 gp41 peptides.
J Neurovirol. 6: 51-60. 17. Gendek-Kubiak, H. and Gendek, E.G. (2004) Immunolocalization of protectin (CD59) and macrophages in polymyositis and dermatomyositis.
J Neuroimmunol. 149: 187-94.

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Cat# ABD12013
(40 western blots)
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