|Application ||IHC-F, FC, IP, Func|
|Other Accession||P31995, P31994|
|Calculated MW||35001 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||K562 cell line.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2|
|Target/Specificity||Mouse anti-Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Clone AT10 blocks the binding of IgG to Fc gamma RII (Larssonet al.1997).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD32 Antibody, clone AT10 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Greenman, J. et al. (1991) Characterisation of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.Mol. Immunol. 28: 1243-1254. 2. van den Herik-Oudijk, I. E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes.J. Immunol. 152: 574-585. 3. Lilliehook, I. et al. (1998) Expression of adhesion and Fc gamma - receptors on canine blood eosinophils and neutophils studied by anti-human monoclonal antibodies. Vet. Immunol. Immunopathol. 61: 181-193. 4. Larsson, M. et al. (1997) Human dendritic cells handling of binding, uptake and degradation of free and IgG - immune complexed dinitrophenylated human serum albumin in vitro.Immunology. 90: 138-146. 5. Dutertre, C- A. et al. (2008) A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function.Journal of Leukocyte Biology. 84: 1511-1519. 6. Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells. Vet Immunol Immunopathol. 135: 188-98. 7. Sims, G.P. et al. (2005) Identification and characterization of circulating human transitional B cells.Blood. 105: 4390-8. 8. Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.J Exp Med. 203: 1629-35. 9. Zhao, X.W. et al. (2011) CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction.Proc Natl Acad Sci U S A. 108 (45): 18342-7. 10. Araújo, M.S.et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.Vet Immunol Immunopathol. 141: 64-75. 11. Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.J Exp Med. 203: 1629-35. 12. Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.J Immunol. 185: 4738-49. 13. Shannon, O. et al. (2010) Platelet activation and biofilm formation by Aerococcus urinae, an endocarditis-causing pathogen.Infect Immun. 78: 4268-75. 14. Ito, T. et al. (1999) A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.J Immunol. 163: 1409-19. 15. Moreira, M. L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytesVeterinary Parasitology. 15 Feb [Epub ahead of print]
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