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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD32 Antibody, clone AT10 (RPE)   

Anti-Human CD32 Antibody, clone AT10

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti-Human CD32 Antibody, clone AT10  ABD12014
    Staining of human peripheral blood monocytes with Mouse anti Human CD32: Biotin
  • FC - Anti-Human CD32 Antibody, clone AT10  ABD12014
    Staining of human peripheral blood granulocytes with Mouse anti Human CD32:FITC
  • FC - Anti-Human CD32 Antibody, clone AT10  ABD12014
    Staining of human peripheral blood granulocytes with Mouse anti Human CD32:RPE
  • FC - Anti-Human CD32 Antibody, clone AT10  ABD12014
    Detection of CD32 in normal human peripheral blood cells using the Bio-Rad S3 Cell Sorter. 1x106 cells were suspended in 100μL buffer and stained with 10μL Mouse anti Human CD32:RPE . Lymphocyte were gated for analysis (A): Histogram overlay with stained cells represented in green and unstained control cells in grey. (B): Density plot of CD32 cells.
  • FC - Anti-Human CD32 Antibody, clone AT10  ABD12014
    Staining of human peripheral blood granulocytes with Mouse anti Human CD32
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, FC, IP, Func
Primary Accession P12318
Other Accession P31995, P31994
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names AT10
Calculated MW 35001 Da
Additional Information
Other Species Dog,Rh,Pig
Purification Purified IgG prepared by affinity chromatography on Protein A
Immunogen K562 cell line.
Shelf Life 18 months from date of despatch.
Gene ID 2212
Other Names Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2
Target/Specificity Mouse anti-Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Clone AT10 blocks the binding of IgG to Fc gamma RII (Larssonet al.1997).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD32 Antibody, clone AT10 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Greenman, J. et al. (1991) Characterisation of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.
Mol. Immunol. 28: 1243-1254. 2. van den Herik-Oudijk, I. E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes.
J. Immunol. 152: 574-585. 3. Lilliehook, I. et al. (1998) Expression of adhesion and Fc gamma - receptors on canine blood eosinophils and neutophils studied by anti-human monoclonal antibodies.
Vet. Immunol. Immunopathol. 61: 181-193. 4. Larsson, M. et al. (1997) Human dendritic cells handling of binding, uptake and degradation of free and IgG - immune complexed dinitrophenylated human serum albumin in vitro.
Immunology. 90: 138-146. 5. Dutertre, C- A. et al. (2008) A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function.
Journal of Leukocyte Biology. 84: 1511-1519. 6. Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
Vet Immunol Immunopathol. 135: 188-98. 7. Sims, G.P. et al. (2005) Identification and characterization of circulating human transitional B cells.
Blood. 105: 4390-8. 8. Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.
J Exp Med. 203: 1629-35. 9. Zhao, X.W. et al. (2011) CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction.
Proc Natl Acad Sci U S A. 108 (45): 18342-7. 10. Araújo, M.S.et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.
Vet Immunol Immunopathol. 141: 64-75. 11. Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.
J Exp Med. 203: 1629-35. 12. Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.
J Immunol. 185: 4738-49. 13. Shannon, O. et al. (2010) Platelet activation and biofilm formation by Aerococcus urinae, an endocarditis-causing pathogen.
Infect Immun. 78: 4268-75. 14. Ito, T. et al. (1999) A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.
J Immunol. 163: 1409-19. 15. Moreira, M. L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes
Veterinary Parasitology. 15 Feb [Epub ahead of print]

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Cat# ABD12014
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