|Application ||IHC-F, FC, IP, Func|
|Other Accession||P31995, P31994|
|Calculated MW||35001 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||K562 cell line.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2|
|Target/Specificity||Mouse anti-Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Clone AT10 blocks the binding of IgG to Fc gamma RII (Larssonet al.1997).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD32 Antibody, clone AT10 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Greenman, J. et al. (1991) Characterisation of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.Mol. Immunol. 28: 1243-1254. 2. van den Herik-Oudijk, I. E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes.J. Immunol. 152: 574-585. 3. Lilliehook, I. et al. (1998) Expression of adhesion and Fc gamma - receptors on canine blood eosinophils and neutophils studied by anti-human monoclonal antibodies. Vet. Immunol. Immunopathol. 61: 181-193. 4. Larsson, M. et al. (1997) Human dendritic cells handling of binding, uptake and degradation of free and IgG - immune complexed dinitrophenylated human serum albumin in vitro.Immunology. 90: 138-146. 5. Dutertre, C- A. et al. (2008) A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function.Journal of Leukocyte Biology. 84: 1511-1519. 6. Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells. Vet Immunol Immunopathol. 135: 188-98. 7. Sims, G.P. et al. (2005) Identification and characterization of circulating human transitional B cells.Blood. 105: 4390-8. 8. Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.J Exp Med. 203: 1629-35. 9. Zhao, X.W. et al. (2011) CD47-signal regulatory protein-α (SIRPα) interactions form a barrier for antibody-mediated tumor cell destruction.Proc Natl Acad Sci U S A. 108 (45): 18342-7. 10. Araújo, M.S.et al. (2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.Vet Immunol Immunopathol. 141: 64-75. 11. Benitez-Ribas, D. et al. (2006) Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake.J Exp Med. 203: 1629-35. 12. Santer, D.M. et al. (2010) C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes.J Immunol. 185: 4738-49. 13. Shannon, O. et al. (2010) Platelet activation and biofilm formation by Aerococcus urinae, an endocarditis-causing pathogen.Infect Immun. 78: 4268-75. 14. Ito, T. et al. (1999) A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.J Immunol. 163: 1409-19. 15. Moreira, M. L. et al. (2016) Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytesVeterinary Parasitology. 15 Feb [Epub ahead of print]
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