|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Horse CD13 antibody, clone CVS19 recognizes the equine CD13 cell surface antigen, also known as Aminopeptidase N, a single-pass type II membrane protein belonging to the peptidase N family with a molecular weight of between 150 - 170 kDa.CD13 is widely expressed by a range of cell types including all blood neutrophils, basophils, monocytes, fibroblasts, kidney epithelial cells, endothelial cells and mesenchymal stem cells, but not by T or B cells. It is involved in a broad spectrum of biological processes and is believed to be linked to a number of disease states including tumor invasion (Saikiet al.1993).Mouse anti-Horse CD13 antibody, clone CVS19 may be used for differentiating myeloid and lymphoid linage cells in tumors of the haematopoietic system.In addition to clone CVS19, other CVS clones recognising equine MHC and cell surface antigens are available.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Horse CD13 Antibody, clone CVS19 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Kydd, J.H. & Antczak, D.F. (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991Equine Immunology 4: 5. 2. Dunkel, B. et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P orneutrophil-platelet aggregate formation. Vet Immunol Immunopathol. 131: 25-32. 3. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.Vet Immunol Immunopathol. 62: 101-143 4. Loftus J.P et al. (2006) Matrix metalloproteinase-9 in laminae of black walnut extract treated horses correlates with neutrophil abundance.Vet Immunol Immunopathol. 113:267-76. 5. Aalberts, M. et al. (2012) Spermatozoa recruit prostasomes in response to capacitation induction.Biochim Biophys Acta.1834: 2326-35. 6. Maia, L. et al. (2013) Immunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow.Microsc Res Tech. 76: 618-24. 7. Radcliffe, C.H. et al. (2010) Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations.Stem Cells Dev. 19: 269-82.
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