|Application ||IHC-F, FC, IP|
|Calculated MW||37682 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A.|
|Immunogen||Human peripheral blood lymphocytes.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD86, CD28LG2|
|Target/Specificity||Mouse anti-Human CD86 antibody, clone Bu63 recognizes human CD86 also known as B7-2, a type I transmembrane protein expressed by monocytes and activated B cells (Engelet al.1994). CD86 acts as a co-stimulaory molecule along with CD80 (Lanieret al.1995) and is a ligand for CD28 and CTLA-4 (Azumaet al.1993).CD86 is a member of the Immunoglobulin superfamily and carries an extracellular domain bearing both an Ig-v-like domain which contains the CTLA-4 binding site and an adjacent C2-like domain. CD86 plays an important role in co-stimulation of T cell proliferation (Freemanet al.1993), IL-2 production (Ribotet al.2012) and in the primary immune response (Schultzeet al.1996).Domain depletion epitope mapping studies indicate that the binding site of Mouse anti-Human CD86, clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeaninet al.1997).Studies suggest that CD86 along with CD80 may be exploited as receptors for adenovirus entry into cells (Shortet al.20042006).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Storage at 4℃ or at -20℃.|
|Precautions||Anti-Human CD86 Antibody, clone BU63 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. McLellan, A. et al. (1999) Induction of dendritic cell costimulator molecule expression is suppressed by T cells in the absence of antigen-specific signalling: role of cluster formation, CD40 and HLA-class II for dendritic cell activation. Immunology. 98: 171-180. 2. Nozawa, Y. et al. (1993) A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells.J. Pathol. 169: 309. 3. Goodyear, O. et al. (2010) Induction of a CD8+ T-cell response to the MAGE cancer testis antigen by combined treatment with azacitidine and sodium valproate in patients with acute myeloid leukemia and myelodysplasia. Blood. 116: 1908-18. 4. Angel, C.E.et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.J. Immunol. 176: 5730-5734. 5. Salte, T. et al. (2010) Increased intracellular growth of Mycobacterium avium in HIV-1 exposed monocyte-derived dendritic cells.Microbes Infect. 13: 276-83. 6. Adler, H.S. et al. (2010) Neuronal nitric oxide synthase modulates maturation of human dendritic cells.J Immunol. 184: 6025-34. 7. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.BMC Immunol. 12:2. 8. Kapsogeorgou, E.K. et al. (2001) Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4.J Immunol. 166: 3107-13. 9. Lozanoska-Ochser, B. et al. (2008) Expression of CD86 on human islet endothelial cells facilitates T cell adhesion and migration.J Immunol. 181: 6109-16. 10. Urban, B.C. et al. (2001) A role for CD36 in the regulation of dendritic cell function.Proc Natl Acad Sci U S A. 98: 8750-5. 11. Zhan, H. et al. (2003) The immunomodulatory role of human conjunctival epithelial cells.Invest Ophthalmol Vis Sci. 44: 3906-10. 12. Sprater, F. et al. (2012) Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic CellsPLoS ONE 7(11): e49577. 13. McCarthy, N.E. et al. (2013) Proinflammatory Vd2+ T Cells Populate the Human Intestinal Mucosa and Enhance IFN-? Production by Colonic aß T Cells.J Immunol. 191: 2752-63. 14. Hofmann-Wellenhof, R. et al. (2004) Sunburn cell formation, dendritic cell migration, and immunomodulatory factor production after solar-simulated irradiation of sunscreen-treated human skin explants in vitro.J Invest Dermatol. 123: 781-7. 15. Rajkovic, I. et al. (2011) Differences in T-helper polarizing capability between human monocyte-derived dendritic cells and monocyte-derived Langerhans'-like cells.Immunology. 132: 217-25.
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