|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Porcine peripheral blood lymphocytes.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Pig CD14, clone MIL2 recognizes porcine CD14. Clone MIL2 was clustered as porcine CD14 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haversonet al.2001) . Clone MIL2 immunoprecipitates a protein of ~50 kDa consistent with the expected apparent molecular weight of porcine CD14, and demonstrates the expected CD14 profile by dual labelling and competition studies. Further, pre-incubation of peripheral blood monocytes with MIL2 inhibits the binding of FITC labelled LPS, consistent with masking the CD14 LPS binding site (Thackeret al.2001) . Mouse anti-pig CD14, clone MIL2 demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry with a similar expression pattern to the anti-human CD14 clone TüK4, lymphocytes and eosinophils are negative for MIL2 staining (Zelnickovaet al.2007). Cloning and characterization of porcine CD14 indicates a high degree of both functional and structural conservation when compared to CD14 from other mammalian species, the gene maps to chromosome 2 and is expressed on a wide range of tissues in a manner consistent with expression on myeloid cells. (Petersenet al.2007,Sanzet al.2007).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Pig CD14 Antibody, clone MIL2 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues.J Immunol Methods. 170 (2): 233-45. 2. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.Vet Immunol Immunopathol. 80 (1-2): 93-109. 3. Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs. FASEB J. 24: 712-22. 4. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.Kidney Int. 58: 838-50. 5. Li, Y. et al. (2014) Identification of apoptotic cells in the thymus of piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus.Virus Res. pii: S0168-1702(14)00173-7. 6. Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.Immunology. 110: 440-9. 7. Vanderheijden, N. et al. (2003) Involvement of sialoadhesin in entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages.J Virol. 77: 8207-15. 8. Barratt-Due, A. et al.(2011)Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.J Immunol. 187: 4913-9. 9. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys.Kidney Int. 62: 654-67. 10. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.J Immunol. 188: 3382-94. 11. Thorgersen, E.B. et al. (2009) Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.Infect Immun. 77: 725-32. 12. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.J Immunol Methods. 327: 18-29. 13. Facci, M.R. et al. (2011) Stability of expression of reference genes in porcine peripheral blood mononuclear and dendritic cells.Vet Immunol Immunopathol. 141: 11-5. 14. Koutná, I. et al. (2012) Flow Cytometry Analysis of Intracellular ProteinIn: Flow Cytometry - Recent Perspectives, Schmid, I. (Ed.), ISBN: 978-953-51- 15. Facci, M.R. et al. (2010) A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs.Immunology. 129: 396-405. 16. Schierack, P. et al. (2009) Effects of Bacillus cereusvar.toyoi on immune parameters of pregnant sows.Vet Immunol Immunopathol.127: 26-37. 17. Lundeland, B. et al. (2011) Severe gunshot injuries in a porcine model: impact on central markers of innate immunity.Acta Anaesthesiol Scand. 55: 28-34. 18. Thorgersen, E.B. et al. (2008) Cyanobacterial LPS antagonist (CyP)-a novel and efficient inhibitor of Escherichia coli LPS-induced cytokine response in the pig.Mol Immunol. 45: 3553-7. 19. Schierack, P. et al.(2007)Bacillus cereusvar.toyoi enhanced systemic immune response in piglets.Vet Immunol Immunopathol. 118: 1-11. 20. Ondrackova, P. et al. (2012) Interaction of porcine neutrophils with different strains of enterotoxigenic Escherichia coli.Vet Microbiol. 160: 108-16. 21. Ondrackova, P. et al. (2012) Phenotypic characterisation of the monocyte subpopulations in healthy adult pigs and Salmonella-infected piglets by seven-colour flow cytometry.Res Vet Sci. pii: S0034-5288(12)00256-1. 22. Vicenova, M. et al. (2014) Evaluation of in vitroandin vivo anti-inflammatory activity of biologically active phospholipids with anti-neoplastic potential in porcine model.BMC Complement Altern Med. 14: 339. 23. Alvarez, B. et al. (2015) Phenotypic and functional heterogeneity of CD169+ and CD163+ macrophages from porcine lymph nodes and spleen.Dev Comp Immunol. 44: 44-9. 27. Egge, K.H. et al. (2015) Organ inflammation in porcine Escherichia coli sepsis is markedly attenuated by combined inhibition of C5 and CD14.Immunobiology. 220 (8): 999-1005. 28. Moffat, L. et al. (2014) Development and characterisation of monoclonal antibodies reactive with porcine CSF1R (CD115).Dev Comp Immunol. 47 (1): 123-8. 29. Nguyen DN et al. (2015) Oral antibiotics increase blood neutrophil maturation and reduce bacteremia and necrotizing enterocolitis in the immediate postnatal period of preterm pigs.Innate Immun. pii: 1753425915615195. 30. Liu J et al. (2016) The Role of Porcine Monocyte Derived Dendritic Cells (MoDC) in the Inflammation Storm Caused by Streptococcus suis Serotype 2 Infection.PLoS One. 11 (3): e0151256.1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.2. Petersen, C.B. et al. (2007) Cloning, characterization and mapping of porcine CD14 reveals a high conservation of mammalian CD14 structure, expression and locus organization.Dev Comp Immunol. 31: 729-37.3. Sanz, G. et al. (2007) Molecular cloning, chromosomal location, and expression analysis of porcine CD14.Dev Comp Immunol. 2007;31(7):738-47.
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