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>   home   >   Products   >   Primary Antibodies   >   Anti-Pig wCD8 alpha Antibody, clone MIL12 (RPE)   

Anti-Pig wCD8 alpha Antibody, clone MIL12 (FITC)

Mouse Anti-Pig Monoclonal Antibody

  • FC - Anti-Pig wCD8 alpha Antibody, clone MIL12 (FITC) ABD12036
    Pig peripheral blood lymphocytes stained with Mouse anti Pig wCD8a followed by Goat anti Mouse IgG (H/L):FITC
  • FC - Anti-Pig wCD8 alpha Antibody, clone MIL12 (FITC) ABD12036
    Published customer image:Mouse anti Pig wCD8 alpha antibody, clone MIL12 used for the evaluation of CD7α expression on porcine lymphocytes by flow cytometry.Image caption:Frequency of leukocyte subsets in PBMC and LPMC using FCM analysis A) shows a representative FCM analysis (n=4) from PBMCs and LPMC isolated from 6 week old pigs were analysed on the frequencies of different immune cell subsets using three-color FCM. Forward scatter and side scatter were used to gate on leukocytes (PBMCs (a) and LPMC (h) for further analysis of myeloid cells (CD172+; b and i), NK cells (CD3- CD8α+; c and j), B cells (CD21+; e and l) and T cells (CD3+; d and k). Gated T cells were then further analysed on their CD4/CD8α expression to determine the frequency of CTLs (CD4-CD8αhigh; f and m) and CD4+ T cells (f and m). CD8α expression of CD4+ T cells was also investigated to define the frequency of experienced CD4+ T cells (CD8a+CD4+; g and n). (o) Summarizes the results of this FCM analysis of all four analysed animals. Percentages from the PBMC population are represented by a closed circle and the percentages from the LPMC populations are represented by an open box. (p) and (q) is a pie chart showing the relative average percentage of PBMCs and LPMCs from all 4 animals.From: Pasternak JA, Ng S, Kaser T, Meurens F, Wilson HL Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model.J Vaccines Vaccin 5:242.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Pig
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names MIL12
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Porcine mesenteric lymphocytes.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Pig wCD8 alpha antibody, clone MIL12 recognizes an epitope on the alpha chain of porcine wCD8. Clone MIl12 was clustered at the Third International Swine CD Workshop (Haversonet al.2001). Clone MIL12 was determined to bind to the CD8a epitope on the alpha chain based on its staining pattern on T lymphocytes and on its ability to block binding of the previously characterized CD8a antibody clone 76-2-11 to T lymphocytes (Saalmulleret al.2001).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Pig wCD8 alpha Antibody, clone MIL12 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
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1. Sarradell, J. et al. (2003) A morphologic and immunohistochemical study of the bronchus-associated lymphoid tissue of pigs naturally infected with Mycoplasma hyopneumoniae.
Vet Pathol. 40: 395-404. 2. Kick, A.R. et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
Res Vet Sci. 91 (3): e68-72. 3. Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.
Cell Biol Int. 36 (12): 1233-8. 4. Cao, D. et al. (2010) Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets.
Vaccine. 28: 6006-13. 5. Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.
Vet Immunol Immunopathol. 104: 131-44. 6. Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia-reperfusion and renal medulla injury.
J Pharmacol Exp Ther. 302 (3): 861-70. 7. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.
Kidney Int. 58: 838-50. 8. Piva, A. et al. (2005) Activated carbon does not prevent the toxicity of culture material containing fumonisin B1 when fed to weanling piglets.
J Anim Sci. 83 (8): 1939-47. 8. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys.
Kidney Int. 62: 654-67. 9. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.
J Anim Sci. 90: 649-56. 10. Shi, K. et al. (2008) Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.
Vet Microbiol. 129: 367-77. 11. Spreeuwenberg, M.A. et al. (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning.
J Nutr. 131: 1520-7. 12. Clapperton, M. et al. (2008) Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance.
Animal. 2: 1575-84. 13. Leifer, I. et al. (2012) Characterization of C-strain"Riems"TAV-epitope escape variants obtained through selective antibody pressure in cell culture.
Vet Res. 43: 33. 14. Tuchscherer, M. et al. (2012) Effects of inadequate maternal dietary protein:carbohydrate ratios during pregnancy on offspring immunity in pigs.
BMC Vet Res. 8: 232. 15. Swamy, H.V. et al. (2003) Effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on growth and immunological measurements of starter pigs, and the efficacy of a polymeric glucomannan mycotoxin adsorbent.
J Anim Sci. 81: 2792-803. 15. Lu, X. et al. (2012) Genome-wide association study for T lymphocyte subpopulations in swine.
BMC Genomics. 13: 488. 16. Monroy-Salazar, H.G. et al. (2012) Effects of a live yeast dietary supplement on fecal coliform counts and on peripheral blood CD4+ and CD8+ lymphocyte subpopulations in nursery pigs.
J Swine Health Prod. 20: 276–282. 17. Ostrowska, E. et al. (2004) Effects of dietary conjugated linoleic acid on haematological and humoral responses in the grower pig.
Australian Journal of Agricultural Research 55: 711–718 18. Carter, D.B. et al. (2002) Phenotyping of transgenic cloned piglets.
Cloning Stem Cells. 4: 131-45. 19. Stenfeldt, C. et al. (2014) Morphologic and phenotypic characteristics of myocarditis in two pigs infected by foot-and mouth disease virus strains of serotypes O or A.
Acta Vet Scand. 56: 42. 20. Zeigler, B.M. et al. (2015) The development and validation of methods for evaluating the immune system in preweaning piglets.
Food Chem Toxicol. 84: 197-207. 21. Pasternak, J. A. (2014) Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model
Journal of Vaccines & Vaccination. 05 (05): . 22. Marinaro M et al. (2015) Changes in peripheral blood leucocytes of sheep experimentally infected with Mycoplasma agalactiae.
Vet Microbiol. 175 (2-4): 257-64.1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.

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Cat# ABD12036
(40 western blots)
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