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Anti-Human CD8 Antibody, clone LT8

Mouse Anti-Human Monoclonal Antibody

  • IHC - Anti-Human CD8 Antibody, clone LT8  ABD12037
    Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System. Low power
  • FC - Anti-Human CD8 Antibody, clone LT8  ABD12037
    Figure A. FITC conjugated mouse anti human CD3 and APC conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and APC conjugated mouse anti human CD8 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock
  • FC - Anti-Human CD8 Antibody, clone LT8  ABD12037
    Published customer image:Mouse anti Human CD8 antibody, clone LT8 used for the evaluation of CD8α exptession on T lymphocytes from marmoset blood, spleen and lung by flow cytometry.Image caption:The percentage of the total leucocyte count for various cell types identified in naive marmosets (a) cells in naïve blood, (b) in naive spleens, and (c) in naive lungs. CD8 T cells are expressed as a percentage of CD3+ cells. Bar represents the median value.From: M. Nelson and M. Loveday, “Exploring the Innate Immunological Response of an Alternative Nonhuman Primate Model of Infectious Disease; the Common Marmoset,”Journal of Immunology Research, vol. 2014, Article ID 913632, 8 pages, 2014.
  • IHC-P - Anti-Human CD8 Antibody, clone LT8  ABD12037
    Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System. Medium power
  • IHC - Anti-Human CD8 Antibody, clone LT8  ABD12037
    Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD8 antibody, clone LT8 followed by Histar Detection System. Medium power
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01732
Other Accession P10966
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names LT8
Calculated MW 25729 Da
Additional Information
Other Species Marmoset,Chimpanzee,Cynomolgus Pr,Red-bellied Tamarin
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Normal human peripheral blood lymphocytes.
Shelf Life 18 months from date of despatch.
Gene ID 925
Other Names T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL
Target/Specificity Mouse anti-Human CD8 antibody, clone LT8 recognizes the human CD8 cell surface glycoprotein expressed by a subset of peripheral blood T cells which express cytotoxic/suppressor activity. It is also expressed weakly on NK cells.The CD8 antigen is a co-receptor for MHC Class I in conjunction with the T cell receptor, and is important in the selection process of CD8+ MHC Class I restricted T cells.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD8 Antibody, clone LT8 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Zarkesh-Esfahani, H. et al. (2001) High-dose Leptin activates human leukocytes via receptor expression on monocytes.
J. Immunol. 167: 4593 - 4599. 2. Manninen, A. et al. (2002) HIV-1 Nef interacts with inositol trisphosphate receptor to activate calcium signaling in T cells.
J. Exp.Med. 195 (8): 1023 -1032. 3. Parnes, J.R. (1989) Molecular biology and function of CD4 and CD8.
Adv. Immunol. 44: 265-311. 4. Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
J Histochem Cytochem. 57: 1159-67. 5. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
BMC Immunol. 12: 2. 6. Nelson, M. et al. (2010) Characterization of lethal inhalational infection with Francisella tularensis in the common marmoset (Callithrix jacchus).
J Med Microbiol. 59: 1107-13. 7. Gibbings, D.J. et al. (2007) CD8 alpha is expressed by human monocytes and enhances Fc gamma R-dependent responses.
BMC Immunol. 8: 12. 8. Junker, A. et al. (2007) Multiple sclerosis: T-cell receptor expression in distinct brain regions.
Brain. 130: 2789-99. 9. Held, K. et al. (2011) Expression of herpes simplex virus 1-encoded microRNAs in human trigeminal ganglia and their relation to local T-cell infiltrates.
J Virol. 85 (19): 9680-5. 10. Hood, S.P. et al. (2013) Changes in immune cell populations in the periphery and liver of GBV-B-infected and convalescent tamarins (Saguinus labiatus).
Virus Res. pii: S0168-1702(13)00396-1. 11. Nelson, M. & Loveday, M. (2014) Exploring the innate immunological response of an alternative nonhuman primate model of infectious disease; the common marmoset.
J Immunol Res. 2014: 913632.

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Cat# ABD12037
(40 western blots)
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