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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Mouse CD45R Antibody, clone RA3-6B2 (RPE-CY7)   

Anti-Mouse CD45R Antibody, clone RA3-6B2 (FITC)

Rat Anti-Mouse Monoclonal Antibody

  • FC - Anti-Mouse CD45R Antibody, clone RA3-6B2 (FITC) ABD12039
    Staining of mouse spleen cells with Rat anti Mouse CD45R:FITC
  • FC - Anti-Mouse CD45R Antibody, clone RA3-6B2 (FITC) ABD12039
    Staining of mouse spleen cells with Rat anti Mouse CD45R: Alexa Fluor® 488
  • IF - Anti-Mouse CD45R Antibody, clone RA3-6B2 (FITC) ABD12039
    Published customer image:Rat anti Mouse CD45R antibody, clone RA3-6B2 used for the enumeration of B cells in the intestinal mucosa by immunofluorescence.Image caption:Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 μg/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistology. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.From: Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE 4: e8291.
  • FC - Anti-Mouse CD45R Antibody, clone RA3-6B2 (FITC) ABD12039
    Staining of mouse spleen cells with Rat anti Mouse CD45R: Alexa Fluor® 647
  • FC - Anti-Mouse CD45R Antibody, clone RA3-6B2 (FITC) ABD12039
    Staining of mouse spleen cells with Rat anti Mouse CD45R:RPE-Alexa Fluor® 647
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06800
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names RA3-6B2
Calculated MW 144604 Da
Additional Information
Other Species H,Cat
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Murine leukaemia-induced pre-B tumour cells (RAW112).
Shelf Life 12 months from date of reconstitution.
Other Names Receptor-type tyrosine-protein phosphatase C,, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5
Target/Specificity Rat anti-Mouse CD45R antibody, clone RA3-6B2 recognizes murine CD45R, a form of the CD45 antigen expressed by B cells and lytically active subsets of NK cells and non-MHC restricted CTL's. Rat anti-Mouse CD45R antibody, clone RA3-6B2 immunoprecipitates the high molecular weight form of CD45 (220kD).Rat anti-Mouse CD45R antibody, clone RA3-6B2 has been reported as being suitable for plp fixed paraffin embedded tissues (Whiteland et al.1995).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Mouse CD45R Antibody, clone RA3-6B2 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Coffman, R.L. (1982) Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development.
Immunol Rev. 69: 5-23. 2. Rosmalen, J.G. et al. (2000) Subsets of macrophages and dendritic cells in nonobese diabetic mouse pancreatic inflammatory infiltrates: correlation with the development of diabetes.
Lab Invest. 80 (1): 23-30. 3. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. 4. Spangrude, G.J. et al. (1988) Purification and characterization of mouse hematopoietic stem cells.
Science. 241: 58-62. 5. Spangrude, G.J. et al. (1988) Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus.
J Exp Med. 167 (5): 1671-83. 6. Holmes, K.L. et al. (1986) Analysis of neoplasms induced by Cas-Br-M MuLV tumor extracts.
J Immunol. 137 (2): 679-88. 7. Monteith, C.E. et al. (1996) Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues.
Can J Vet Res. 60 (3): 193-8. 8. Lundqvist, J. et al. (2010) Concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis.
Infect Immun. 78 (5): 1924-30. 9. Herrmann, I. et al. (2006) Streptococcus pneumoniae Infection aggravates experimental autoimmune encephalomyelitis via Toll-like receptor 2.
Infect Immun. 74: 4841-8. 10. Kleiter, I. et al. (2010) Smad7 in T cells drives T helper 1 responses in multiple sclerosis and experimental autoimmune encephalomyelitis.
Brain. 133: 1067-81. 11. Lacroix-Lamande, S. et al. (2009) Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation.
PLoS One. 4: e8291. 12. Bertilaccio, M.T. et al. (2011) Lack of TIR8/SIGIRR triggers progression of chronic lymphocytic leukemia in mouse models.
Blood. 118: 660-9. 13. Gengozian, N. et al. (2005) Characterization of a monoclonal antibody identifying a CD45RA antigen on feline leukocytes.
Vet Immunol Immunopathol. 108: 253-64. 14. Giuriato, S. et al. (2010) Conditional TPM3-ALK and NPM-ALK transgenic mice develop reversible ALK-positive early B-cell lymphoma/leukemia.
Blood. 115: 4061-70. 15. Hawke, S. et al. (1998) Long-term persistence of activated cytotoxic T lymphocytes after viral infection of the central nervous system.
J Exp Med. 187: 1575-82. 16. Nakaya, T. et al. (2010) Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression.
J Immunol. 185: 5180-7. 17. Perry, M.J. et al. (2000) Effects of high-dose estrogen on murine hematopoietic bone marrow precede those on osteogenesis.
Am J Physiol Endocrinol Metab. 279: E1159-65. 18. Ruf, M.T. et al. (2012) Chemotherapy-associated changes of histopathological features of Mycobacterium ulcerans lesions in a Buruli ulcer mouse model.
Antimicrob Agents Chemother. 56 (2): 687-96. 19. Soejima, M. et al. (2011) Role of innate immunity in a murine model of histidyl-transfer RNA synthetase (Jo-1)-mediated myositis.
Arthritis Rheum. 63: 479-87. 20. Stevenson, P.G. et al. (2002) Uncoupling of virus-induced inflammation and anti-viral immunity in the brain parenchyma.
J Gen Virol. 83: 1735-43. 21. Fanning, S. et al. (2012) Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protection.
Proc Natl Acad Sci U S A. 109 (6): 2108-13. 22. Ruf, M.T. et al. (2012) Chemotherapy-Associated Changes of Histopathological Features of Mycobacterium ulcerans Lesions in a Buruli Ulcer Mouse Model.
Antimicrob Agents Chemother. 56: 687-96. 23. Mori, H. et al. (2015) Introduction of Zinc-salt Fixation for Effective Detection of Immune Cell-related Markers by Immunohistochemistry.
Toxicol Pathol. pii: 0192623315587593.

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Cat# ABD12039
(40 western blots)
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