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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Mouse CD25 Antibody, clone PC61.5.3 (RPE)   

Anti-Mouse CD25 Antibody, clone PC61.5.3

Rat Anti-Mouse Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, FC, IP, Func
Primary Accession P01590
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG1
Clone Names PC61.5.3
Calculated MW 30723 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen B6.1 CTL cell line
Shelf Life 18 months from date of despatch.
Gene ID 16184
Other Names Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25, Il2ra, Il2r
Target/Specificity Rat anti-Mouse CD25 antibody, clone PC61.5.3 reacts with the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Rat anti-Mouse CD25 antibody, clone PC61.5.3 is reported to inhibit IL-2 binding and IL-2 dependent proliferation.
Preservative & Stabilisers Preservative Free
Storage Store at -20℃ only.
PrecautionsAnti-Mouse CD25 Antibody, clone PC61.5.3 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Moreau, J.L. et al. (1987) Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site.
Eur J Immunol. 17 (7): 929-35. 2. Hashimoto, N. et al. (1986) Dissociation of interleukin 2-dependent and -independent B cell proliferation with monoclonal anti-interleukin 2 receptor antibody.
Eur J Immunol. 16 (3): 317-20. 3. Lowenthal, J.W. et al. (1985) High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61.
J Immunol. 135 (6): 3988-94. 4. Ceredig, R. et al. (1985) Expression of interleukin-2 receptor as a differentiation marker on intrathymic stem cells.
Nature 314: 98-100. 5. Yaqoob, P. and Calder, P.C. (1997) Glutamine Requirement of Proliferating T lymphocytes.
Nutrition 13: 646-651. 6. Scotland, R.S. et al. (2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.
Blood. 118 (22): 5918-27.

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Cat# ABD12041
(40 western blots)
Availability: Inquire
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