|Application ||FC, IP, CHIP|
|Calculated MW||25077 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||Spleen and bone marrow cells from CE mice|
|Shelf Life||18 months from date of despatch.|
|Other Names||Killer cell lectin-like receptor subfamily B member 1C, CD161 antigen-like family member C, Lymphocyte antigen 55c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C, Natural killer cell surface protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c|
|Target/Specificity||Mouse anti-Mouse CD161 / NK1.1 antibody, clone PK136 recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. The NK1.1 surface antigen is also known as CD161b/CD161c and Ly-55.In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.Mouse anti-Mouse NK1.1 Antigen antibody, clone PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Mouse anti-Mouse NK1.1 antibody, clone PK136 has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD161 / NK1.1 Antibody, clone PK136 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Koo, G.C. and Peppard, J.R. (1981) Establishment of monoclonal anti-NK-1.1 antibody.Hybridoma 3: 301-303. 2. Koo, G.C. et al. (1986) The NK-1.1 (-) mouse: A model to study differentiation of murine NK cells.J. Immunol. 137: 3742-3737. 3. Kung, S.K.P. et al. (1999) The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells.J. Immunol. 162: 5876-5887. 4. Carlyle, J.R. et al. (1999) Mouse NKR-P1B, a novel NK1.1 antigen with inhibitory function. J.Immunol. 162: 5917-5923. 5. Carnemolla, B. et al. (2002) Enhancement of the antitumor properties of interleukin-2 by its targeted delivery to the tumor blood vessel extracellular matrix.Blood. 99: 1659-65. 6. Carpentier, A.F. et al. (1999) Oligodeoxynucleotides containing CpG motifs can induce rejection of a neuroblastoma in mice.Cancer Res. 59: 5429-32. 7. Sakai, T. et al. (2010) Inflammatory disease and cancer with a decrease in Kupffer cell numbers in Nucling-knockout mice.Int J Cancer. 126: 1079-94. 8. Svensson, L. et al. (2003) gammadelta T cells contribute to the systemic immunoglobulin E response and local B-cell reactivity in allergic eosinophilic airway inflammation.Immunology. 108: 98-108. 9. Hazlett, L.D. et al. (2007) NKT cells are critical to initiate an inflammatory response after Pseudomonas aeruginosa ocular infection in susceptible mice.J Immunol. 179 : 1138-46. 10. Joseph-Pietras, D. et al. (2006) Anti-tumoural activity of peripheral blood mononuclear cells against melanoma cells: discrepant in-vitro and in-vivo effects.Melanoma Res. 16: 325-33.
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