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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD4 Antibody, clone RPA-T4 (APC)   

Anti-Human CD4 Antibody, clone RPA-T4

Mouse Anti-Human Monoclonal Antibody

     
  • IHC - Anti-Human CD4 Antibody, clone RPA-T4  ABD12044
    Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. High power
  • FC - Anti-Human CD4 Antibody, clone RPA-T4  ABD12044
    Figure A. FITC conjugated mouse anti human CD3 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti human CD3 and RPE conjugated mouse anti human CD4 . All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes in the presence of Human SeroBlock
  • IHC - Anti-Human CD4 Antibody, clone RPA-T4  ABD12044
    Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Medium power
  • IHC-P - Anti-Human CD4 Antibody, clone RPA-T4  ABD12044
    Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Low power
  • IHC - Anti-Human CD4 Antibody, clone RPA-T4  ABD12044
    Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Medium power
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, FC
Primary Accession P01730
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names RPA-T4
Calculated MW 51111 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Human PHA blasts.
Shelf Life 12 months from date of reconstitution.
Gene ID 920
Other Names T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, CD4
Target/Specificity Mouse anti-human CD4 antibody, clone RPA-T4 recognizes human CD4, a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1, of the extracellular region, of the CD4 molecule.Mouse anti-human CD4 antibody, clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation, see Piatier-Tonneau et al, 1997 for details. AbD Serotec recommend the use of Mouse anti-Human CD4:Low Endotoxin (ABD12044EL) for this purpose.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Storage at 4℃ or at -20℃.
PrecautionsAnti-Human CD4 Antibody, clone RPA-T4 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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References

1. Zarkesh-Esfahani, H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes.
J. Immunol.167:4593 – 4599. 2. Voehringer, D. et al. (2002) Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectin-like receptor G1 (KLRG1).
Blood.100: 3698 - 3702. 3. Piatier-Tonneau, D. (1997) CD4 workshop panel report.
In: Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998. 4. Penton-Rol, G. et al. (2011) C-Phycocyanin ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells.
Int Immunopharmacol. 11: 29-38. 5. Wright, G.J. et al. (2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.
Immunology. 102: 173-9. 6. Zhang, Y. et al. (2013) Accelerated In Vivo Proliferation of Memory Phenotype CD4+ T-cells in Human HIV-1 Infection Irrespective of Viral Chemokine Co-receptor
PLoS Pathog 9(4): e1003310.

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Cat# ABD12044
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