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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Human CD39 Antibody, clone A1 (RPE)   

Anti-Human CD39 Antibody, clone A1 (FITC)

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti-Human CD39 Antibody, clone A1 (FITC) ABD12045
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD39:Low Endotoxin followed by Rabbit F(ab')2 anti Mouse IgG:FITC
  • FC - Anti-Human CD39 Antibody, clone A1 (FITC) ABD12045
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD39 followed by Rabbit F(ab')2 anti Mouse IgG:FITC
  • FC - Anti-Human CD39 Antibody, clone A1 (FITC) ABD12045
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD39:FITC
  • FC - Anti-Human CD39 Antibody, clone A1 (FITC) ABD12045
    Published customer image:Mouse anti human CD39, clone A1 used for the evaluation of CD39 expression on cultured mononuclear cells by flow cytometry.Image caption:Phenotype and functional analysis of Foxp3hi and Foxp3int T cells in direct co-cultures or separated through a transwell. T cells from direct or transwell-separated iRBC co-cultures were harvested on day 6 of culture and either re-stimulated with PMA/ionomycin for intracellular cytokine staining, or phenotyped directly. Foxp3hi:Foxp3int ratios and the percentage of IFN? or IL-4 producing cells within the CD25hiFoxp3int population were determined for 5 donors. Values for transwell-separated T cells (black bars) are expressed as fold change following normalization onto values in direct co-cultures (white bars) for each donor (Mean+/-SEM) and compared by paired Student's t-test (A). Freshly isolated CD25-Foxp3- and CD25+Foxp3+ CD4+ T cells (day 0) and CD4+CD25-Foxp3-, Foxp3int and Foxp3hi CD4+CD25hi T cells from direct or transwell separated cultures (day 6) from 3 donors were phenotyped for a wide range of markers, and expression levels for each surface marker within each population were normalized on isotype control staining, when analysed as median fluorescence intensity . Expression levels of surface markers on CD4+CD25-Foxp3- cells were comparable between conditions for each donor (data not shown). Thus, to compare the phenotype of day 0 CD25+Foxp3+ nTregs, and day 6 induced CD25hiFoxp3int and CD25hiFoxp3hi cells (black bars), expression levels are expressed as fold change (Mean+/-SEM) compared to CD4+CD25-Foxp3- cells (white bars, relative level = 1) for each donor and condition (B). Experiments were set up with a 2:1 iRBC:PBMC ratio.From: Scholzen A, Mittag D, Rogerson SJ, Cooke BM, Plebanski M Plasmodium falciparum–Mediated Induction of Human CD25hiFoxp3hi CD4 T Cells Is Independent of Direct TCR Stimulation and Requires IL-2, IL-10 and TGFß.PLoS Pathog 5(8): e1000543.
  • FC - Anti-Human CD39 Antibody, clone A1 (FITC) ABD12045
    Published customer image:Mouse anti human CD39, clone A1 used for the evaluation of CD39 expression on cultured mononuclear cells by flow cytometry.Image caption:Phenotype of Foxp3hi and Foxp3int CD4+CD25hi T cells induced by malaria-infected RBCs. After 8 days of iRBC:PBMC co-culture, immuno magnetic selection was used to isolate CD4+CD25hi (black line) and CD25- cells (grey filled). CD25 and Foxp3 expression in the two sorted populations was analysed by flow cytometry (A). mRNA levels for Foxp3, TGFβ1 and IL-10 were normalized on 18SrRNA levels for each donor. Data from 8 donors (Mean+/-SEM) are shown, presented as relative mRNA levels normalized on mRNA levels in CD4+CD25- T cells for each donor (A). PBMC of 3 donors were phenotyped on day 0 prior to iRBC-PBMC co-culture set up, and after 6 days of iRBC:PBMC co-culture (2:1 ratio). Dot plots show the gating strategies for Foxp3 expressing cells on day 0 and day 6, respectively (B). Representative histograms depicting isotype control staining (grey filled) and specific staining for various surface markers on CD25-Foxp3- and CD25hi Foxp3-expressing CD4 T cells (black line) from each respective culture are shown (B).From: Scholzen A, Mittag D, Rogerson SJ, Cooke BM, Plebanski M Plasmodium falciparum–Mediated Induction of Human CD25hiFoxp3hi CD4 T Cells Is Independent of Direct TCR Stimulation and Requires IL-2, IL-10 and TGFß.PLoS Pathog 5(8): e1000543.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession P49961
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names A1
Calculated MW 57965 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen PHA activated human lymphocytes
Shelf Life 18 months from date of despatch.
Gene ID 953
Other Names Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, 3.6.1.5, Ecto-ATP diphosphohydrolase 1, Ecto-ATPDase 1, Ecto-ATPase 1, Ecto-apyrase, Lymphoid cell activation antigen, CD39, ENTPD1, CD39
Target/Specificity Mouse anti-Human CD39, clone A1 recognizes the human CD39 cell surface antigen, a ~70-100 kDa molecule expressed on peripheral blood B cells, T cells and monocytes, and weakly expressed by granulocytes.CD39 has intrinsic ecto-ATPase activity (Wanget al.1996), and expression can be induced on T cells and increased on B cells, as a late activation antigen (Maliszewskiet al.1994).Mouse anti-Human CD39, clone A1 has been shown to block MHC independent target cell recognition by hapten-specific CTL (Scholzenet al.2009).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin
Storage Store at +4℃ or -20℃.
PrecautionsAnti-Human CD39 Antibody, clone A1 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Aversa, G.G. et al. (1988) Detection of a late lymphocyte activation marker by A1, A new monoclonal antibody.
Transpl. Proc. 20: 49 - 52 2. Waugh, J.A. et al. (1989) Staining of normal and rejecting kidney using the activation panel.
In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al.Oxford University Press. p485. 3. Aversa, G.G. and Hall, B.M. (1989) Activation panel antigen expression on PBL activated by PHA or in MLR.
In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al. Oxford University Press, p.498. 4. Aversa, G.G. et al. (1989) Use of monoclonal antibodies to study in vivoandin vitro activated lymphocytes.
Transpl. Proc. 21: 349 - 350 5. Stein, H. et al. (1989) Activated Section report.
In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al. Oxford University Press, p.387. 6. Suranyi, M.G. et al. (1991) Lymphocyte adhesion molecules in T cell mediated lysis of kidney cells.
Kidney International. 39: 312 - 319 7. Stockl, J. et al. (2001) Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1 restricted, hapten-specific CTL.
J. Immunol. 167: 2724-2733. 8. Scholzen, A. et al. (2009) Plasmodium falciparum-mediated induction of human CD25Foxp3 CD4 T cells is independent of direct TCR stimulation and requires IL-2, IL-10 and TGFbeta.
PLoS Pathog. 5: e1000543. 9. Borsellino, G. et al. (2007) Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression
Blood. 110:1225-32. 10. Mittag, D. et al. (2010) The effector T cell response to ryegrass pollen is counterregulated by simultaneous induction of regulatory T cells.
J Immunol. 184: 4708-16. 11. Loeuillet, C. et al. (2008) In vitro whole-genome analysis identifies a susceptibility locus for HIV-1.
PLoS Biol. 6: e32. 12. Rawstron, A.C. et al. (2010) Chronic lymphocytic leukaemia (CLL) and CLL-type monoclonal B-cell lymphocytosis (MBL) show differential expression of molecules involved in lymphoid tissue homing.
Cytometry B Clin Cytom. 78 Suppl 1: S42-6. 13. Alam, M.S. et al. (2009) CD73 is expressed by human regulatory T helper cells and suppresses proinflammatory cytokine production and Helicobacter felis-induced gastritis in mice.
J Infect Dis. 199: 494-504. 14. Moreno-Fernandez, M.E. et al. (2011) Regulatory T cells control HIV replication in activated T cells through a cAMP-dependent mechanism.
Blood. 117: 5372-80. 15. Guevara-Flores, A. et al. (2008) 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP-diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci.
Mol Biochem Parasitol. 162: 123-33. 16. Glenn, J.R. et al. (2008) Raised levels of CD39 in leucocytosis result in marked inhibition of ADP-induced platelet aggregation via rapid ADP hydrolysis.
Platelets. 19: 59-69. 17. Häusler SF et al. (2014) Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion.
Am J Transl Res. 6 (2): 129-39.

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