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Anti-Human CD100 Antibody, clone A8

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti-Human CD100 Antibody, clone A8  ABD12046
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD100:APC
  • E - Anti-Human CD100 Antibody, clone A8  ABD12046
    Published customer image:Mouse anti Human CD100 antibody, clone A8 used for the detection of CD100 by ELISA and flow cytometry.Image caption:172.4 recognizes CD100, a 150 kDa protein expressed as a homodimer. (A) Surface radioiodinated YTS cell lysate was immunoprecipitated with mAb 172.4. The immunoprecipitate was analyzed first on non-reducing and then on reducing SDS-PAGE. The left lane is an aliquot of immunoprecipitated proteins resolved under reducing conditions. Molecular weight markers are as shown. The two forms of CD100 (150 and 120 kDa respectively) are indicated with arrows. (B, D) ELISA plates were coated with CD100-Ig and assayed for binding to the indicated antibodies as described in “Materials and Methods”. (C) ELISA plates were coated with CD99-Ig and assayed for binding to the indicated antibodies as described in “Materials and Methods”. The background binding to BSA was subtracted. Figure shows one representative experiment out of four performed. (E) IL-2 activated NK line was stained with either 172.4 or the commercial anti human CD100 antibody A8. FITC conjugated Goat F(ab') anti mouse IgG antibody was used as secondary Ab. Gray histogram is staining with secondary antibody only. Figure show one representative experiment out of 5 performed.From: Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O CD100 on NK Cells Enhance IFN? Secretion and Killing of Target Cells Expressing CD72.PLoS ONE 2(9): e818. doi:10.1371/journal.pone.0000818.
  • WB - Anti-Human CD100 Antibody, clone A8  ABD12046
    Published customer image:Mouse anti Human CD100 antibody, clone A8 used for the detection of CD100 by western blotting.Image caption:The interaction between CD100 and CD72 leads to the phosphorylation of serine residues on proteins associate with CD100. (A) 35S-labeled activated NK cells were incubated for 20 minutes with adherent BW or BW/CD72 cells. The wells were washed, the remaining cells were lysed and the level of radioactivity was measured in CPM units (counts per mint). The results presented after subtraction of NK cells only. (B) 35S-labeled activated NK cells were incubated for 20 minutes with adherent BW/CD72 cells that were pre incubated with CD100-Ig or CD99-Ig for 2 hr prior to the incubation with NK cells. The wells were washed, the remaining cells were lysed and the level of radioactivity was measured. *p?=?0.02. (C) Activated NK cells were incubated with target cells (BW or BW/CD72), in 37°C, for the indicated time periods. Cells were lysed and proteins were immunoprecipitated by mAb 172.4. The immunoprecipitated proteins were separated on a reducing SDS-PAGE. Phosphorylated proteins were detected in western blot by using rabbit anti phospho-serine polyclonal Ab. CD100 levels were detected in western blot by using the A8 anti human CD100. (D) Densitometrical analysis of the level of phosphorylation on serine residues of the three proteins indicated by arrows in Figure 5C. The levels of phosphorylation are relative to their level at time zero which was set as one. Figure shows one representative experiment out of two performed.From: Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O CD100 on NK Cells Enhance IFN? Secretion and Killing of Target Cells Expressing CD72.PLoS ONE 2(9): e818. doi:10.1371/journal.pone.0000818.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IP
Primary Accession Q92854
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names A8
Calculated MW 96150 Da
Additional Information
Other Species Cynomolgus Pr,Rh
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Human PHA activated lymphocytes.
Shelf Life 18 months from date of despatch.
Gene ID 10507
Other Names Semaphorin-4D, A8, BB18, GR3, CD100, SEMA4D, C9orf164, CD100, SEMAJ
Target/Specificity Mouse anti-Human CD100 antibody, clone A8 recognizes human Sepaphorin 4D, also known as CD100, A8, BB18 or GR3. CD100 is a 841 amino acid ~150 kDa homodimeric single pass type 1 transmembrane cell surface glycoprotein actin as a receptor for some members of the plexin family. CD100 is expressed at low levels by peripheral blood T cells, and at higher density on activated T cells and B cells, it is also expresses at high levels in fetal kidney with lower levels in fetal brain, liver and lung (Maestriniet al.1996).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD100 Antibody, clone A8 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Aversa, G.G. et al. (1989) Use of monoclonal antibodies to study in vivoandin vitro-activated lymphocytes.
Transplant Proc. 21 (1 Pt 1): 349-50. 2. Van Vliet, S. J. et al. (1995) Species cross reactivity (human-monkey-bovine) of the adhesion structure section mAb.
Leucocyte Typing V. Oxford University Press p1607-1608. 3. Mizrahi, S. et al. (2007) CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72.
PLoS. One. 2: e818. 4. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Exp. Anim. 49: 97-110.

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