|Application ||WB, FC, IP|
|Calculated MW||96150 Da|
|Other Species||Cynomolgus Pr,Rh|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Human PHA activated lymphocytes.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Semaphorin-4D, A8, BB18, GR3, CD100, SEMA4D, C9orf164, CD100, SEMAJ|
|Target/Specificity||Mouse anti-Human CD100 antibody, clone A8 recognizes human Sepaphorin 4D, also known as CD100, A8, BB18 or GR3. CD100 is a 841 amino acid ~150 kDa homodimeric single pass type 1 transmembrane cell surface glycoprotein actin as a receptor for some members of the plexin family. CD100 is expressed at low levels by peripheral blood T cells, and at higher density on activated T cells and B cells, it is also expresses at high levels in fetal kidney with lower levels in fetal brain, liver and lung (Maestriniet al.1996).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD100 Antibody, clone A8 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Aversa, G.G. et al. (1989) Use of monoclonal antibodies to study in vivoandin vitro-activated lymphocytes.Transplant Proc. 21 (1 Pt 1): 349-50. 2. Van Vliet, S. J. et al. (1995) Species cross reactivity (human-monkey-bovine) of the adhesion structure section mAb. Leucocyte Typing V. Oxford University Press p1607-1608. 3. Mizrahi, S. et al. (2007) CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72.PLoS. One. 2: e818. 4. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp. Anim. 49: 97-110.
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