|Application ||IHC-F, FC, E, IP|
|Calculated MW||109540 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Human AML cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Aminopeptidase N, AP-N, hAPN, 184.108.40.206, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13, ANPEP, APN, CD13, PEPN|
|Target/Specificity||Mouse anti-Human CD13 antibody, clone WM15 recognizes human CD13 also known as aminopeptidase N. CD13 is a single pass type II glycosylated integral membrane protein with a predicted molecular mass of ~110 kDa and an apparent molecular mass of ~150 kDa expressed by granulocytes, monocytes, fibroblasts, endothelial cells and by myeloid leukaemia cells (Bradstocket al.1985). CD13 acts as a major cell surface receptor for group 1 coronoviruses (Breslinet al.2003) which bind to a critical sequence encompassing amino acid residies 288-295 (Kolbet al.1997).CD13 functions as an aminopeptidase enzyme, a metalloprotease present as both a membrane bound form and also a soluble aminopeptidase N.Mouse anti-Human CD13, clone WM15 inhibits infection of cells by human coronavirus (Lachanceet al.1998) but not hepatitis C virus (Koutsoudakiset al.2006) and inhibits aminopeptidase N activity of the CD13 molecule (Asmunet al.1992)|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD13 Antibody, clone WM15 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Bradstock, K.F. et al. (1985) Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells.Pathology. 17 (3): 392-9. 2. Bradstock, K.F. et al. (1985) Myeloid progenitor surface antigen identified by monoclonal antibody.Br J Haematol. 61 (1): 11-20. 3. Favaloro, E.J. et al. (1988) Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33.Br J Haematol. 69 (2): 163-71. 4. Favaloro, E.J. (1991) CD-13 (gp150; aminopeptidase-N): co-expression on endothelial and haemopoietic cells with conservation of functional activity.Immunol Cell Biol. 69 ( Pt 4): 253-60. 5. Favaloro, E.J. et al. (1993) The hepatobiliary disease marker serum alanine aminopeptidase predominantly comprises an isoform of the haematological myeloid differentiation antigen and leukaemia marker CD-13/gp150.Clin Chim Acta. 220 (1): 81-90. 6. Favaloro, E.J. et al. (1993) CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated.Exp Hematol. 21 (13): 1695-701. 7. Tavoosidana, G. et al. (2011) Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancer.Proc Natl Acad Sci U S A. 108: 8809-14. 8. Gredmark, S. et al. (2004) Human Cytomegalovirus Induces Inhibition of Macrophage Differentiation by Binding to Human Aminopeptidase N/CD13J Immunol. 173: 4897-907 9. Grzywacz, B. et al. (2011) Natural killer-cell differentiation by myeloid progenitors.Blood. 117: 3548-58. 10. Stolzing, A. et al. (2008) Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies.Mech Ageing Dev. 129: 163-73. 11. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.J Biomed Biotechnol. 2012:172420. 12. Negussie, A.H. et al. (2010) Synthesis and in vitro evaluation of cyclic NGR peptide targeted thermally sensitive liposome.J Control Release. 143: 265-73. 13. Lassnig, C. et al. (2005) Development of a transgenic mouse model susceptible to human coronavirus 229E.Proc Natl Acad Sci U S A. 102 (23): 8275-80. 14. Thielitz, A. et al. (2004) Identification of extra- and intracellular alanyl aminopeptidases as new targets to modulate keratinocyte growth and differentiation.Biochem Biophys Res Commun. 321 (4): 795-801. 15. McCormack, E. et al. (2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.Blood. 121 (7): e34-42.
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