|Application ||WB, IHC-F, FC, IP|
|Calculated MW||39825 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Human AML cells|
|Shelf Life||18 months from date of despatch.|
|Other Names||Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33, CD33, SIGLEC3|
|Target/Specificity||Mouse anti-Human CD33 antibody, clone WM53 recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin. Mouse anti-Human CD33 antibody, clone WM53 immunoprecipitates a protein of ~75kDa from myeloid cells, a smaller protein of approximately 67kDa has been observed in immunoprecipitates from lymphoid targets.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD33 Antibody, clone WM53 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Favaloro, E.J. et al. (1987) Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, gp67 (CD-33).Dis Markers. 5 (4): 215-25. 2. Favaloro, E.J. et al. (1988) Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33.Br J Haematol. 69 (2): 163-71. 3. Hernández-Caselles, T. et al. (2006) A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.J Leukoc Biol. 79: 46-58. 4. Biedermann, B. et al. (2006) Analysis of the CD33-related siglec family reveals that Siglec-9 is an endocytic receptor expressed on subsets of acute myeloid leukemia cells and absent from normal hematopoietic progenitors.Leuk Res. 31: 211-20. 5. Lajaunias, F. et al. (2005) Constitutive repressor activity of CD33 on human monocytes requires sialic acid recognition and phosphoinositide 3-kinase-mediated intracellular signaling.Eur J Immunol. 35: 243-51. 6. Pietschmann, P. et al. (2000) Surface markers and transendothelial migration of dendritic cells from elderly subjects.Exp Gerontol. 35: 213-24. 7. Favaloro, E.J. et al. (1993) Differential expression of surface antigens on activated endothelium.Immunol Cell Biol. 71:571-81. 8. Yasukawa, T. et al. (2012) Simple detection of surface antigens on living cells by applying distinct cell positioning with negative dielectrophoresis.Anal Chem. 84 (20): 8830-6.
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