|Application ||WB, FC, E, IP|
|Calculated MW||18696 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Immunogen||113 amino acid synthetic peptide corresponding to the cytoplasmic domain of murine zeta TCR subunit.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ|
|Target/Specificity||Mouse anti-Human CD247 antibody, clone G3 recognizes the human T cell receptor zeta chain, also known as CD247. The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events.Mouse anti-Human CD247 antibody, clone G3 is capable of precipitating both non-phosphorylated and tyrosine phosphorylated TCR zeta chain and may be used to detect TCR zeta expressing cells using flow cytometry on permeabilised cells.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD247 Antibody, clone G3 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. van Oers, N.S. et al. (1994) Production and characterization of monoclonal antibodies specific for the murine T cell receptor zeta chain.J Immunol Methods. 170 (2): 261-8. 2. Anderson, P. (1995) mAb reative with lymphocyte restricted intracellular antigens. Leucocyte Typing V p325, Oxford University Press.
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