|Application ||WB, IHC-F, FC, E|
|Calculated MW||20641 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Recombinant Mouse IL-10|
|Shelf Life||18 months from date of despatch.|
|Other Names||Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, Il10, Il-10|
|Target/Specificity||Rat anti-Mouse Interleukin-10 antibody, clone JES5-2A5 recognizes mouse Cytokine synthesis inhibitory factor (CSIF), also known as interleukin-10 (IL-10). IL-10 is produced by Ly-1(CD5) expressing B cells (O'Garraet al.1992) and a subset of stimulated T cells (Assemanet al.1999).IL-10 has a wide range of biological properties, including the inhibition of macrophage and T helper cell mediated cytokine synthesis, and the suppression of the delayed hypersensitivity response.Rat anti-Mouse Interleukin-10 antibody, clone JES5-2A5 is reported to neutralize the activity of murine IL-10 (Weber-Nordtet al.1994).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse Interleukin-10 Antibody, clone JES5-2A5 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunological Review 127: 5-24. 2. Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostainingJ. Immunol. Methods. 166: 201-14. 3. Guesdon, J.L. et al. (1979) The use of avidin-biotin interaction in immunoenzymatic techniques.J. Histochem. Cytochem. 27: 1131-1139. 4. Nishigori, C. et al. (1998) Hind III liposomes suppress delayed-type hypersensitivity responses in vivo and induce epidermal IL-10 in vitro.J. Immunol. 161:2684-2691. 5. Weber-Nordt, R. et al. (1994) Lipopolysaccharide-dependent induction of IL-10 receptor expression on murine fibroblasts.J. Immunol. 153:3734-3744. 6. Rennick, D. et al. (1995) Cofactors are essential for stem cell factor-dependent growth and maturation of mast cell progenitors: comparative effects of interleukin-3 (IL-3), IL-4, IL-10, and fibroblasts.Blood. 85: 57-65. 7. Sacco, S. et al. (1997) Protective effect of a single Interleukin-12 (IL-12) predose against the toxicity of subsequent chronic IL-12 in mice: Role of cytokines and glucocorticoids.Blood. 90:4473-4479. 8. Biswas, S.K. et al. (2006) A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).Blood. 107: 2112-22. 9. Gaupp, S. et al. (2008) Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines.Am J Pathol. 173: 119-29. 10. Gonzalez-Juarrero, M. et al. (2013) Polar Lipids of Burkholderia pseudomallei Induce Different Host Immune Responses.PLoS One. 8: e80368.
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