|Calculated MW||76189 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Activated, Lewis rat derived microglial cells.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Platelet endothelial cell adhesion molecule, PECAM-1, CD31, Pecam1, Pecam|
|Target/Specificity||Mouse anti-Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009). This clone has been reported as suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilsonet al.2007). Mouse anti-Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Macleanet al.2001)|
|Preservative & Stabilisers||0.09% Sodium Azide; 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat CD31 Antibody, clone TLD-3A12 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Williams, K.C. et al. (1996) PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat.J. Neurosci. Res. 45(6):747-57. 2. Nakao, A. et al. (2003) Carbon monoxide inhalation protects rat intestinal grafts from ischemia/reperfusion injury.Am J Pathol. 163: 1587-98. 3. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreaticadult progenitor cell populations.Stem Cells Dev. 18: 1389-98. 4. Ott, I. et al. (2005) Endothelial-like cells expanded from CD34+ blood cells improve left ventricular function after experimental myocardial infarction.FASEB J. 19: 992-4. 5. Fujimoto, K.L. et al. (2007) An elastic, biodegradable cardiac patch induces contractile smooth muscle and improves cardiac remodeling and function in subacute myocardial infarction.J Am Coll Cardiol. 49: 2292-300. 6. Thebault, P. et al. (2010) The C-type lectin-like receptor CLEC-1, expressed by myeloid cells and endothelial cells, is up-regulated by immunoregulatory mediators and moderates T cell activation.J Immunol. 183: 3099-108. 7. Graham, M.J. et al. (1998) In vivo distribution and metabolism of a phosphorothioate oligonucleotide within rat liver after intravenous administration.J Pharmacol Exp Ther. 286: 447-58. 8. Haywood, L. et al. (2003) Inflammation and angiogenesis in osteoarthritis.Arthritis Rheum. 48: 2173-7. 9. Kielian, T. and Hickey, W.F. (2010) Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development.Am J Pathol. 157: 647-58. 10. Lochhead, J.J. et al. (2010) Oxidative stress increases blood-brain barrier permeability and induces alterations in occludin during hypoxia-reoxygenation.J Cereb Blood Flow Metab. 30: 1625-36. 11. Arkudas, A. et al. (2007) Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model.Mol Med. 13: 480-7. 12. Nakao, A. et al.(2011)Ex vivo carbon monoxide delivery inhibits intimal hyperplasia in arterialized vein grafts.Cardiovasc Res. 89: 457-63. 13. Ohnishi, T. et al. (2007) Comparison of endothelial cell proliferation in normal liver and adipose tissue in B6C3F1 mice, F344 rats, and humans.Toxicol Pathol. 35: 904-9. 14. Schilte, M.N. et al. (2009) Long-term intervention with heparins in a rat model of peritoneal dialysis.Perit Dial Int. 29: 26-35. 15. Seegers, H.C. et al. (2003) Enhancement of angiogenesis by endogenous substance P release and neurokinin-1 receptors during neurogenic inflammation.J Pharmacol Exp Ther. 306: 8-12. 16. Wilson, E. et al. (2007) An evaluation of the immunohistochemistry benefits of boric acid antigen retrieval on rat decalcified joint tissues.J Immunol Methods. 322: 137-42. 17. Willis, C.L. et al. (2010) Protein kinase C activation modulates reversible increase in cortical blood-brain barrier permeability and tight junction protein expression during hypoxia and posthypoxic reoxygenation.J Cereb Blood Flow Metab. 30: 1847-59. 18. Salehi-Had, H. et al. (2011) Utilizing targeted gene therapy with nanoparticles binding alpha v beta 3 for imaging and treating choroidal neovascularization.PLoS One. 6: e18864. 19. MacLean, A.G. et al. (2001) Rhesus macaque brain microvessel endothelial cells behave in a manner phenotypically distinct from umbilical vein endothelial cells.J Neuroimmunol. 118: 223-32. 20. Ceelen, W. et al. (2007) Recombinant human erythropoietin alpha modulates the effects of radiotherapy on colorectal cancer microvessels.Br J Cancer. 96: 692-700. 21. Tung, H.C. et al. (2015) The Beneficial Effects of P2X7 Antagonism in Rats with Bile Duct Ligation-induced Cirrhosis.PLoS One. 10 (5): e0124654. 22. Oboshi, M. et al. (2015) Temporary dietary iron restriction affects the process of thrombus resolution in a rat model of deep vein thrombosis.PLoS One. 10 (5): e0126611. 23. Wu, S.H. et al. (2015) Autologous adipose-derived stem cells attenuate muscular atrophy and protect spinal cord ventral horn motor neurons in an animal model of burn injury.Cytotherapy. 17 (8): 1066-75. 24. Ikutomi, M. et al. (2015) Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation.J Mol Cell Cardiol. 86: 121-35. 25. Ferrantelli Evelina et al. (2016) The dipeptide alanyl-glutamine ameliorates peritoneal fibrosis and attenuates IL-17 dependent pathways during peritoneal dialysisKidney International. Jan 8 [Epub ahead of print] 26. Lux, M. et al. (2016) In vitro maturation of large-scale cardiac patches based on a perfusable starter matrix by cyclic mechanical stimulation.Acta Biomater. 30: 177-87. 27. Liu, T. et al. (2015) A study of the relationship of metabolic MR parameters to estrogen dependence in breast cancer xenografts.NMR Biomed. 28 (9): 1087-96. 28. Saito, K. et al. (2015) Responses of pulp vasculature after cavity preparation in rat molarsJournal of Oral Biosciences. 57 (3): 157-64. 29. Kakaiy, A. et al. (2015) Comparing protective effect of grape seed extract versus atorvastatin on endometriosis in rat model: Evidence for immunohistochemical and biochemical alterations.Vet Res Forum. 6 (2): 101-10. 30. Brandl, A. et al. (2014) A novel early precursor cell population from rat bone marrow promotes angiogenesis in vitro.BMC Cell Biol. 15: 12. 31. Sun, C.K. et al. (2015) Mixed serum-deprived and normal adipose-derived mesenchymal stem cells against acute lung ischemia-reperfusion injury in rats.Am J Transl Res. 7 (2): 209-31. 32. Matsugami, H.et al. (2014) VEGF secretion by adipose tissue-derived regenerative cells is impaired under hyperglycemic conditions via glucose transporter activation and ROS increase Biomedical Research. 35 (6): 397-405. 33. Park; J.R. et al. (2016) Effects of Peroxisome Proliferator-Activated Receptor-δ Agonist on Cardiac Healing after Myocardial Infarction.PLoS One. 11 (2): e0148510.
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