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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD120a Antibody, clone H398    

Anti-Human CD120a Antibody, clone H398

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti-Human CD120a Antibody, clone H398  ABD12061
    Staining of human peripheral blood granulocytes with Mouse anti human CD120a: Alexa Fluor® 647
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19438
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names H398
Calculated MW 50495 Da
Additional Information
Other Species Rb
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Purified human tumour necrosis factor receptor type 1.
Shelf Life 12 months from date of reconstitution.
Gene ID 7132
Other Names Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1, Tumor necrosis factor receptor type I, TNF-RI, TNFR-I, p55, p60, CD120a, Tumor necrosis factor receptor superfamily member 1A, membrane form, Tumor necrosis factor-binding protein 1, TBPI, TNFRSF1A, TNFAR, TNFR1
Target/Specificity Mouse anti-Human CD120a antibody, clone H398 recognizes an extracellular domain of the 55 kDa human TNF receptor (p55, TNF-R1, CD120a). No binding occurs to the 75 kDa TNF receptor (CD120b). CD120a is weakly expressed by monocytes and granulocytes.Mouse anti-Human CD120a antibody, clone H398 may be used to detect high levels of TNFR1, in western blotting under reducing conditions, such as recombinant material, but it is not suitable for detection of TNFR1 in normal cells in this application.Mouse anti-Human CD120a antibody, clone H398 inhibits the biological activity of both natural and recombinant human TNFalpha and TNFbeta (Thomaet al.1990andDriet al.1999).Mouse anti-Human CD120a antibody, clone H398 is routinely tested in flow cytometry on human peripheral blood monocytes.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Storage at 4℃ or at -20℃.
PrecautionsAnti-Human CD120a Antibody, clone H398 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Thoma, B. et al. (1990) Identification of a 60-kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses.
J. Exp. Med. 172: 1019-1023. 2. Menegazzi, R. et al. (1994) Evidence that tumor necrosis factor alpha (TNF)-induced activation of neutrophil respiratory burst on biologic surfaces is mediated by the p55 TNF receptor.
Blood 84: 287-293. 3. Dri, P. et al. (1999) Role of the 75-kDa TNF receptor in TNF-induced activation of neutrophil respiratory burst.
J. Immunol. 162: 460-466. 4. Kohrgruber, N. et al. (1999) Survival, maturation, and function of CD11c- and CD11c+ peripheral blood dendritic cells are differentially regulated by cytokines.
J Immunol. 163:3250-3259. 5. Weigart, N. et al. (1996) Gastrin secretion from primary cultures of rabbit antral G cells; stimulation by inflammatory cytokines.
Gasteroenterology. 110: 147 - 154. 6. Kennedy, G. et al. (2010) Biochemical and vascular aspects of pediatric chronic fatigue syndrome.
Arch Pediatr Adolesc Med. 164: 817-23. 7. Gregory, A.P. et al. (2012) TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis.
Nature. 488 (7412): 508-11. 8. Schett, G. et al. (2003) TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response.
Cell Death Differ. 10: 1126-36. 9. Buckley, C.D. et al. (2005) Identification of a phenotypically and functionally distinct population of long-lived neutrophils in a model of reverse endothelial migration.
J Leukoc Biol.79: 303-11. 10. Thiery, J. et al. (2003) Potentiation of a tumor cell susceptibility to autologous CTL killing by restoration of wild-type p53 function.
J Immunol. 170: 5919-26. 11. Lin, K.H. et al. (2009) Mechanisms of resveratrol-induced platelet apoptosis.
Cardiovasc Res. 83: 575-85. 12. Baker, P.K. et al. (2012) Response of hairy cells to IFN-alpha involves induction of apoptosis through autocrine TNF-alpha and protection by adhesion.
Blood. 100: 647-53. 13. Nakamura-Lopez, Y. et al. (2015) RSV P-protein impairs extrinsic apoptosis pathway in a macrophage-like cell line persistently infected with respiratory syncytial virus.
Virus Res. 204: 82-7.

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Cat# ABD12061
(40 western blots)
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