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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Mouse CD117 Antibody, clone 2B8 (RPE)   

Anti-Mouse CD117 Antibody, clone 2B8

Rat Anti-Mouse Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05532
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names 2B8
Calculated MW 109343 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G.
Immunogen IL-3 dependent mast cells.
Shelf Life 18 months from date of despatch.
Gene ID 16590
Other Names Mast/stem cell growth factor receptor Kit, SCFR,, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117, Kit, Sl
Target/Specificity Rat anti-Mouse CD117 antibody, clone 2B8 recognizes mouse CD117, a 150kDa cell surface molecule that is otherwise known as the Kit, or c-kit, receptor.Rat anti-Mouse CD117 antibody, clone 2B8 recognizes an epitope within the extracellular region of CD117. In mice, CD117 is predominantly expressed on multipotent haematopoietic stem cells from the adult bone marrow and foetal liver.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse CD117 Antibody, clone 2B8 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Ikuta, K. and Weissman, I.L. (1992) Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation.
Proc. Nat. Acad. Sci. USA 89: 1502-1506. 2. Fleming, W.H. et al. (1993) Steel factor influences the distribution and activity of murine hematopoietic stem cells in vivo.
Proc. Natl. Acad. Sci. USA 90: 3760-3764. 3. Rico-Vargas, S.A. et al. (1994) c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody
J. Immunol. 152: 2845-2852. 4. Manova, K. et al. (1992) c-kit receptor and ligand expression in postnatal development of the mouse cerebellum suggests a function for c-kit in inhibitory interneurons.
J. Neurosci. 12: 4663-4676. 5. Maeda, H. et al. (1992) Requirement of c-kit for development of intestinal pacemaker system.
Development 116: 369-375. 6. Takagi, M. et al. (1992) Stimulation of mouse connective tissue-type mast cells by hemopoietic stem cell factor, a ligand for the c-kit receptor.
J. Immunol. 148: 3446-3453. 7. de Vrics, P. et al. (1992) Thymus reconstitution by c-kit expressing hematopoietic stem cells purified from adult mouse bone marrow.
J. Exp. Med. 176: 1503-1509. 8. Yoshinaga, K. et al. (1991) Role of c-kit in mouse spermatogenesis: identification of spermatogonia as a specific site of c-kit expression and function.
Development 113: 689-699.

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Cat# ABD12064
(40 western blots)
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