Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Mouse CD11c Antibody, clone N418 (RPE)   

Anti-Mouse CD11c Antibody, clone N418

Hamster Anti-Mouse Monoclonal Antibody

     
  • FC - Anti-Mouse CD11c Antibody, clone N418  ABD12067
    Published customer image:Hamster anti Mouse CD11c antibody, clone N418 for the evaluation of CD11c expression on murine pulmonary CD8+ve T cells following RSV infection.Image caption:Expression of CD11c on pulmonary CD8+ T cells following RSV infection. Following primary RSV infection lungs, spleens, MLN and blood were harvested from BALB/c mice at the time points indicated. Cells were stained with antibodies to CD3, CD8, and CD11c, CD11b or F4/80. Dot plots show expression of CD11c and CD8 on cells gated for CD3+ lymphocytes harvested from A) lungs, B) spleen, MLN and blood. Numbers indicate the percentage of CD8+ cells. Quadrant settings were adjusted that less than 1% of CD8+ cells stained positive for the isotype control of anti-CD11c-mAb. C) Changes in expression of CD11c, CD11b and F4/80 on CD3+ CD8+ cells from lungs, spleen, MLN, and blood following RSV infection. Cells were stained with antibodies as indicated and analyzed by FACS. The percentage of CD3+8+ cells staining positive for CD11c (black squares), CD11b (open circles) or F4/80 (triangles) after subtraction of isotype controls is shown as mean ± SEM (n = 6, *p < 0.05, significant difference versus dpi 0).From: Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J. The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.Respir Res. 2005 Jul 12;6:70.
  • FC - Anti-Mouse CD11c Antibody, clone N418  ABD12067
    Published customer image:Hamster anti Mouse CD11c antibody, clone N418 used for the evaluation od CD11c expression on dendritic cells by flow cytometryImage caption:Frequency distributions of purified dendritic cells labelled CD11c-FITC showing 97.17% purity , and FITC-isotype control . The information shown is from a single cell isolation procedure, representative of various separate experiments.From: Ponce LV, Corado J, Díaz NL, Tapia FJ. Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis.Kinetoplastid Biol Dis. 2005 Jan 25;4(1):2.
  • IF - Anti-Mouse CD11c Antibody, clone N418  ABD12067
    Published customer image:Hamster anti Mouse CD11c antibody, clone N418 used for the identification of CD11c expressing cells in murine cornea by immunofluorescence.Image caption:Distribution and morphology of labeled cells in the murine cornea at 24 h after intravitreal injection of fluorophore-conjugated reagents. A decreasing density of positive cells is observed from peripheral to central cornea (A,D: peripheral cornea, B,E: paracentral cornea, C,F: central cornea. A-C: MHC-II+ cells, D-F: OVA+ cells). Two populations of each antibody labeled cells, i.e., dendriform (G: MHC-II+ cell, I: CD11c+ cells) and round or irregular shapes (H: MHC-II+ cell, I: CD11c+ cells, J: F4/80+ cell, K: B7-1+ cell) are identified. F4/80+ cells seem to be more elongated in the peripheral area (L), slightly ramose in the paracentral region (M), and more round or oblong in the central cornea (N). All OVA+ cells show round, oblong or irregular shapes (O).From: Meng Q, Yang P, Jin H, Rosenbaum JT, Li B, Zhang H, Zhou H, Huang X, Planck SR. Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection. Mol Vis. 2007 Mar28;13:475-86.
  • FC - Anti-Mouse CD11c Antibody, clone N418  ABD12067
    Published customer image:Hamster anti Mouse CD11c antibody, clone N418 for the evaluation of CD11c expression on murine pulmonary CD8+ve T cells following RSV infection.Image caption:Differential expression of surface markers on pulmonary CD11c+ and CD11c- CD8+ T cells. Lungs of naive mice or on day 8 after RSV infection were harvested. Cells were stained with antibodies for CD8, CD11c, and the surface markers indicated. A) Dot plots show expression of CD11c versus the indicated marker on cells gated for lymphocyte size and CD8 expression after RSV infection. Numbers indicate the percentage of cells in corresponding quadrants. Quadrants were set according to isotype controls except for CD11a, CD54 and CD62L. Since these three molecules are expressed to a considerable degree on naïve CD8 T cells, this expression was used for quadrant settings to highlight differences in CD11+ CTL. B) Histograms show expression of CD54, CD62L, and CD69 on pulmonary CD8+ cells from naïve (dotted line) or RSV infected mice (CD11c+ cells: black line, CD11c- cells: filled histogram). .From: Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J. The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.Respir Res. 2005 Jul 12;6:70.
  • IF - Anti-Mouse CD11c Antibody, clone N418  ABD12067
    Published customer image:Hamster anti Mouse CD11c antibody, vlone N418 used for the detection of CD11c expressing cells in murine adipose tissue by immunofluorescence.Image caption:Neonatal overnutrition exacerbates HFD-induced adipose tissue inflammation. A: Representative images of F4/80 immunoreactivity (red fluorescence) in the epididymal adipose tissue of NL and SL mice fed a chow diet. B: Representative images of F4/80 immunoreactivity (red fluorescence) in the epididymal, retroperitoneal, and inguinal adipose tissue of NL and SL mice fed a high-fat diet . C: Quantification of crown-like structures in the epididymal , retroperitoneal , and inguinal adipose tissue of adult NL and SL mice fed a HFD (n = 9–13 per group from = 6 litters). D: Representative scatterplots showing CD301 and CD11c heterogeneity in CD45+CD64+ macrophages from eWAT of adult NL and SL fed a chow or a HFD. E: Histological illustration of antibodies used for flow cytometry. F: Quantification of CD45+CD64+ total macrophages, CD45+CD64+CD11c+ M1 macrophages and CD45+CD64+CD301+ M2 macrophages in eWAT of SL and NL fed a chow or a HFD (n = 7–8 per group from = 4 litters). *P
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, IF, FC, IP
Primary Accession Q9QXH4
Reactivity Mouse
Host Hamster
Clonality Monoclonal
Isotype IgG
Clone Names N418
Calculated MW 129151 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Immunogen Mouse spleen dendritic cells.
Shelf Life 18 months from date of despatch.
Gene ID 16411
Other Names Integrin alpha-X, CD11 antigen-like family member C, Leukocyte adhesion glycoprotein p150, 95 alpha chain, Leukocyte adhesion receptor p150, 95, CD11c, Itgax
Target/Specificity Hamster anti-Mouse CD11c antibody, clone N418 recognizes the murine homolog of human CD11c, a 150/90 kDa member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells.Hamster anti-Mouse CD11c antibody, clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo(Wanget al.2000).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse CD11c Antibody, clone N418 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.

References

1. Crowley, M.T. et al. (1990) Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen.
J Immunol Methods. 133 (1): 55-66. 2. Metlay, J.P. et al. (1990) The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies.
J Exp Med. 171 (5): 1753-71. 3. Wang, H. et al. (2000) Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization.
Proc Natl Acad Sci U S A. 97 (2): 847-52. 4. Lundqvist, J. et al. (2010) Concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis.
Infect Immun. 78 (5): 1924-30. 5. Beyer, M. et al. (2005) The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.
Respir Res. 6: 70. 6. Goupil, M. et al. (2009) Macrophage-mediated responses to Candida albicans in mice expressing the human immunodeficiency virus type 1 transgene.
Infect Immun. 77: 4136-49. 7. Linehan, S.A.. (2005) The mannose receptor is expressed by subsets of APC in non-lymphoid organs.
BMC Immunol. 6:4. 8. Bjorck, P. (2004) Dendritic cells exposed to herpes simplex virus in vivo do not produce IFN-alpha after rechallenge with virus in vitro and exhibit decreased T cell alloreactivity.
J Immunol. 172: 5396-404. 9. Dahlen, E. et al. (1998) Dendritic cells and macrophages are the first and major producers of TNF-alpha in pancreatic islets in the nonobese diabetic mouse.
J Immunol. 160: 3585-93. 10. de Jersey, J. et al. (2002) Activation of CD8 T cells by antigen expressed in the pituitary gland.
J Immunol. 169: 6753-9. 11. Dimier-Poisson, I. et al. (2003) Protective mucosal Th2 immune response against Toxoplasma gondii by murine mesenteric lymph node dendritic cells.
Infect Immun. 71: 5254-65. 12. Gonzalez-Juarrero, M. and Orme, I.M. (2001) Characterization of murine lung dendritic cells infected with Mycobacterium tuberculosis.
Infect Immun. 69: 1127-33. 13. Hamada, H. et al. (2002) Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine.
J Immunol. 168: 57-64. 14. Meng, Q. et al. (2007) Phenotypes, distribution, and morphological features of antigen-presenting cells in the murine cornea following intravitreal injection.
Mol Vis. 13: 475-86. 15. Mercier, S. et al. (2002) Distinct roles of adenovirus vector-transduced dendritic cells, myoblasts, and endothelial cells in mediating an immune response against a transgene product.
J Virol. 76: 2899-911. 16. Moos, M.P. et al. (2005) The lamina adventitia is the major site of immune cell accumulation in standard chow-fed apolipoprotein E-deficient mice
Arterioscler Thromb Vasc Biol. 25: 2386-91. 17. Nunez, R. et al. (1999) Immortalized cell lines derived from mice lacking both type I and type II IFN receptors unify some functions of immature and mature dendritic cells.
Immunol Cell Biol. 77: 153-63. 18. Ponce, L.V. et al. (2005) Adoptive transfer of dendritic cells modulates immunogenesis and tolerogenesis in a neonatal model of murine cutaneous leishmaniasis.
Kinetoplastid Biol Dis. 4: 2. 19. Zhang, L. et al. (2011) The inflammatory changes of adipose tissue in late pregnant mice.
J Mol Endocrinol. 47 (2): 157-65. 20. Donaldson, D.S. et al. (2012) M cell-depletion blocks oral prion disease pathogenesis.
Mucosal Immunol. 5: 216-25. 21. Wada, T. et al. (2013) Eplerenone ameliorates the phenotypes of metabolic syndrome with NASH in liver-specific SREBP-1c Tg mice fed high-fat and high-fructose diet.
Am J Physiol Endocrinol Metab. 305 (11): E1415-25. 22. Kayser, B.D. et al. (2015) Perinatal Overnutrition Exacerbates Adipose Tissue Inflammation Caused by High-Fat Feeding in C57BL/6J Mice.
PLoS One. 10 (3): e0121954. 23. Kan, M.J. et al. (2015) Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
J Neurosci. 35 (15): 5969-82.

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Cat# ABD12067
Size:
Quantity:
Availability: Inquire
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions