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>   home   >   Products   >   Primary Antibodies   >   Anti-Histidine Tag Antibody, clone AD1.1.10   

Anti-Histidine Tag Antibody, clone AD1.1.10

  • WB - Anti-Histidine Tag Antibody, clone AD1.1.10  ABD12069
    Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin and visualised with Streptavidin : HRP
  • WB - Anti-Histidine Tag Antibody, clone AD1.1.10  ABD12069
    Western blot of HIS tagged protein probed with Mouse anti Histidine tag:Dylight® 800 . Visualised using a LI-COR Odyssey® infrared imaging system
  • FC - Anti-Histidine Tag Antibody, clone AD1.1.10  ABD12069
    Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody
  • WB - Anti-Histidine Tag Antibody, clone AD1.1.10  ABD12069
    Western blot of HIS-tagged protein probed with Mouse anti Histidine tag visualised with Rabbit anti Mouse IgG : HRP
  • WB - Anti-Histidine Tag Antibody, clone AD1.1.10  ABD12069
    His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names AD1.1.10
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen PAX6 transcription factor linked to histidine tag.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian (Hoffmannet al.2007) and Hwanget al.2008) and non-mammalian (Zhenget al.2007;Gunnarsenet al.2010; and de Vooght et al. 2012) cell lines.In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Histidine Tag Antibody, clone AD1.1.10 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Els Conrath, K. et al. (2001) Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs.
J Biol Chem. 276 (10): 7346-50.
2. Suen, J.L. et al. (2001) Characterization of self-T-cell response and antigenic determinant of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1mice.
Immunol. 103: 301-309.
3. Hoffmann, S.C. et al. (2007) Identification of CLEC12B, an inhibitory receptor on myeloid cells.
J Biol Chem. 282 (31): 22370-5.
4. Zheng, J. et al. (2007) Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen.
BMC Immunol. 8: 4.
5. Bahi, A. & Dreyer, J.L. (2008) Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization.
Genes Brain Behav. 7 (2): 244-56.
6. Wrighton, K.H. et al. (2009) Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors.
J Biol Chem. 284 (15): 9755-63.
7. Diefenbacher, M. et al. (2011) The Dsl1 Tethering Complex Actively Participates in Soluble NSF (N-Ethylmaleimide-sensitive Factor) Attachment Protein Receptor (SNARE) Complex Assembly at the Endoplasmic Reticulum in Saccharomyces cerevisiae.
J Biol Chem. 286: 25027-38.
8. Alvarez, M.M. et al. (2010) Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method.
PLoS One. 5: e10176.
9. Bahi, A. et al. (2008) The role of tissue-type plasminogen activator system in amphetamine-induced conditional place preference extinction and reinstatement.
Neuropsychopharmacology. 33: 2726-34.
10. Gunnarsen, K.S. et al. (2010) Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA.
BMC Biotechnol. 10: 8.
11. Hwang, H.Y. et al. (2008) Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment.
Mol Immunol. 45: 2570-80.
12. De Vooght, L. et al. (2012) Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly.
Microb Cell Fact. 11: 23.
13. Saerens, D. et al. (2004) Single domain antibodies derived from dromedary lymph node and peripheral blood lymphocytes sensing conformational variants of prostate-specific antigen.
J Biol Chem. 279 (50): 51965-72.
14. Than, N.G. et al. (2014) Evolutionary origins of the placental expression of chromosome 19 cluster galectins and their complex dysregulation in preeclampsia.
Placenta. 35: 855-65.

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Cat# ABD12069
(40 western blots)
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