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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Mouse TNF Alpha Antibody, clone MP6-XT3    

Anti-Mouse TNF Alpha Antibody, clone MP6-XT3

Rat Anti-Mouse Monoclonal Antibody

  • E - Anti-Mouse TNF Alpha Antibody, clone MP6-XT3  ABD12072
    Recombinant Mouse TNF alpha detected in a sandwich assay with Rat anti Mouse TNF alpha as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin as the detection antibody and Streptavidin:HRP as the final layer
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06804
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG1
Clone Names MP6-XT3
Calculated MW 25896 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Recombinant murine TNF-alpha expressed in E. Coli.
Shelf Life 18 months from date of despatch.
Gene ID 21926
Other Names Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnf, Tnfa, Tnfsf2
Target/Specificity Rat anti-Mouse TNF alpha antibody, clone MP6-XT3 recognizes mouse tumor necrosis factor alpha (TNF alpha), also known as TNFSF2. This 17kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role.Rat anti-Mouse TNF alpha antibody, clone MP6-XT3 is reported to neutralize the effects of mouse TNF alpha (Baumanet al.2003).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse TNF Alpha Antibody, clone MP6-XT3 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples
Immunol. Rev. 127: 5-24. 2. Bauman. S. et al. (2003) Effects of tumor necrosis factor alpha on dendritic cell accumulation in lymph nodes draining the immunization site and the impact on the anticryptococcal cell-mediated immune response.
Infect. Immun. 71:68 - 74. 3. Hostager BS, & Bishop GA. (2002) Role of TNF receptor-associated factor 2 in the activation of IgM secretion by CD40 and CD120b.
J Immunol. 168: 3318-22. 4. Huffnagle GB, et al. (1996) Afferent phase production of TNF-alpha is required forthe development of protective T cell immunity to Cryptococcus neoformans.
J Immunol. 157: 4529-36.

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Cat# ABD12072
(40 western blots)
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