|Application ||WB, FC, E|
|Calculated MW||25896 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Recombinant murine TNF-alpha expressed in E. Coli.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnf, Tnfa, Tnfsf2|
|Target/Specificity||Rat anti-Mouse TNF alpha antibody, clone MP6-XT3 recognizes mouse tumor necrosis factor alpha (TNF alpha), also known as TNFSF2. This 17kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role.Rat anti-Mouse TNF alpha antibody, clone MP6-XT3 is reported to neutralize the effects of mouse TNF alpha (Baumanet al.2003).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse TNF Alpha Antibody, clone MP6-XT3 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samplesImmunol. Rev. 127: 5-24. 2. Bauman. S. et al. (2003) Effects of tumor necrosis factor alpha on dendritic cell accumulation in lymph nodes draining the immunization site and the impact on the anticryptococcal cell-mediated immune response.Infect. Immun. 71:68 - 74. 3. Hostager BS, & Bishop GA. (2002) Role of TNF receptor-associated factor 2 in the activation of IgM secretion by CD40 and CD120b.J Immunol. 168: 3318-22. 4. Huffnagle GB, et al. (1996) Afferent phase production of TNF-alpha is required forthe development of protective T cell immunity to Cryptococcus neoformans.J Immunol. 157: 4529-36.
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