|Application ||IHC-F, FC, E|
|Calculated MW||25896 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Recombinant murine TNF alpha expressed in E. coli.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, Tnf, Tnfa, Tnfsf2|
|Target/Specificity||Rat anti-Mouse TNF alpha antibody, clone MP6-XT22 recognizes mouse tumor necrosis factor alpha (TNF alpha), a ~17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immuno-regulatory role.Rat anti-Mouse TNF alpha antibody, clone MP6-XT22 is reported to neutralize the effects of mouse TNF alpha (Gaupet al.2008andYanget al.1995).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse TNF Alpha Antibody, clone MP6-XT22 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Hostager, B.S. & Bishop, G.A. (2002) Role of TNF receptor-associated factor 2 in the activation of IgM secretion by CD40 and CD120b.J Immunol. 168 (7): 3318-22. 2. Gholami, M. et al. (2015) Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats.Biol Trace Elem Res. 164 (2): 205-11. 3. Ross, S.E. et al. (1997) Suppression of TNF-alpha expression, inhibition of Th1 activity, and amelioration of collagen-induced arthritis by rolipram.J Immunol. 159 (12): 6253-9. 4. Gaupp, S. et al. (2008) Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines.Am J Pathol. 173: 119-29. 5. Yang, J. et al. (1995) Involvement of natural killer cells in nitric oxide production by spleen cells after stimulation with Mycobacterium bovis BCG. Study of the mechanism of the different abilities of viable and killed BCG.J Immunol. 155 (12): 5728-35. 6. Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.Curr Alzheimer Res. 6: 531-40. 7. Nahrendorf, M. et al. (2007) The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.J Exp Med. 204: 3037-47. 8. Jordan, M.B. et al. (2004) An animal model of hemophagocytic lymphohistiocytosis (HLH): CD8+ T cells and interferon gamma are essential for the disorder.Blood. 104: 735-43. 9. Hanada, T. et al. (2006) IFNgamma-dependent, spontaneous development of colorectal carcinomas in SOCS1-deficient mice.J Exp Med. 203: 1391-7. 10. Lee, Y.L. et al. (2003) Oral administration of Agaricus blazei (H1 strain) inhibited tumor growth in a sarcoma 180 inoculation model.Exp Anim. 52: 371-5. 11. Ramakrishna, C. et al. (2004) Differential regulation of primary and secondary CD8+ T cells in the central nervous system.J Immunol. 173: 6265-73. 12. O'Dea, K.P. et al. (2011) Regulation of monocyte subset proinflammatory responses within the lung microvasculature by the p38 MAPK/MK2 pathway.Am J Physiol Lung Cell Mol Physiol. 301: L812-21. 1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.Immunol Rev. 127: 5-24.
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