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>   home   >   Products   >   Primary Antibodies   >   Anti-Rat CD2 Antibody, clone OX-34 (RPE)   

Anti-Rat CD2 Antibody, clone OX-34 (Biotin)

Mouse Anti-Rat Monoclonal Antibody

     
  • IF - Anti-Rat CD2 Antibody, clone OX-34 (Biotin) ABD12080
    Published customer image:Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 fusion proteins inDrosophila by immunofluorescence.Image caption:Ey and Toy are redundantly required to activate stg-FMW. (A) The pattern of conservation of stg-FMW enhancer sequence in Drosophila species as displayed by the UCSC genome browser (http://genome.ucsc.edu) is shown at the top. Two highly conserved regions harboring binding sites for Pax6, BS1 and BS2, and Hth are shown below. (B) Logo of the optimized PAX6 Position Weight Matrix (PWM) used in this study. Nucleotide preference is represented by the height of the letter. (C) Partial sequence of BS1 and BS2. BS1 contains binding sites for So and Pax6 genes (Ey/Toy) highlighted in blue and red, respectively. BS1*: Mutated version of BS1. Ey/Pax6 mutated bases are shown in lowercase. BS2 contains adjacent binding sites for Pax6 (Ey/Toy) and Hth, highlighted in red and green respectively. Mutated core bases for Pax6 (Ey/Toy) and Hth are shown in lowercase in BS2*. (D—G) Analysis of the expression of stg-FMW upon mutation of BS1 or BS2. Representative L3 eye imaginal discs carrying the wild type stg-FMW (D), and the mutated versions stg*BS1 (E) and stg*BS2 (F) of the enhancer. (G) Expression of GFP is not affected upon mutation of BS1 or BS2 in stg-FMW. Simultaneous mutation of BS1 and BS2 (stg*BS1+*BS2) abolishes GFP expression in the eye field; small GFP dots can be detected but only at the dorsal and ventral margins of the eye disc. Discs are outlined by the white dashed lines. The position of the MF is indicated by the white arrow and yellow dashed line. (H—J) Clones of ey (H) or toy (I) RNAi, in the eye imaginal disc. Clones are outlined and marked by the absence of CD2 (red). Expression of stg-FMW (green) is not affected by downregulation of Ey or Toy. (J) Eye disc containing ey and toy double mutant clones, stained for CD2 (red) and stg-FMW (green). In clones of cells double mutant for ey and toy the expression of stg-FMW is lost. White arrow indicates the position of the MF. In all images anterior is to the left.From: Lopes CS, Casares F Eye Selector Logic for a Coordinated Cell Cycle Exit. PLoS Genet 11: e1004981.
  • IF - Anti-Rat CD2 Antibody, clone OX-34 (Biotin) ABD12080
    Published customer image:Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 fusion proteins inDrosophila by immunofluorescence.Image caption:Activation of the Dpp or the Hh pathway does not lead to precocious activation of stg-FMW. Clones of gain of function of an activated form of thickveins (A-C) and Cubitus interruptus (D) showing that the Dpp and Hh signalling pathways do not suffice for activation of stg-FMW in other domains of the eye imaginal disc. Clones are labelled by the absence of CD2 . The activity of stg-FMW is detected by the expression of GFP . Clones are outlined. Anterior is to the left.From: Lopes CS, Casares F Eye Selector Logic for a Coordinated Cell Cycle Exit. PLoS Genet 11(2): e1004981.
  • IF - Anti-Rat CD2 Antibody, clone OX-34 (Biotin) ABD12080
    Published customer image:Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 fusion proteins inDrosophila by immunofluorescence.Image caption:Hth defines the anterior domain of stg-FMW expression. (A) Clone of cells ectopically expressing Hth-HA, labeled in magenta, and stained for stg-FMW . (A’) Expression of stg-FMW is only repressed within the anterior domain of the clone. (B) Eye disc containing clones of a null allele of hth , stained for CD2 and stg-FMW . Clone cells are labeled by the absence of CD2. Within the clone stg-FMW expression occurs in a few cell rows anterior to its normal domain of expression. Arrow indicates premature expression of GFP. The vertical dashed line limits the most anterior expression of stg-FMW in wild-type tissue. The horizontal line represents the width of stg-FWW expression in wild-type tissue. (C) Mutation of the Hth BS (stg*Hth) does not affect the spatial or temporal expression of stg-FMW.From: Lopes CS, Casares F Eye Selector Logic for a Coordinated Cell Cycle Exit. PLoS Genet 11(2): e1004981.
  • IF - Anti-Rat CD2 Antibody, clone OX-34 (Biotin) ABD12080
    Published customer image:Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 tagged fusion protein in Drosophila by immunofluorescence.Image caption:Protrusion formation during mesoderm flattening. (A,B) Two-photon time-lapse sequence of mesoderm cells expressing GFP-Actin in the period following EMT (ventral imaging protocol; see Fig. S1 in the supplementary material). VM, ventral midline; DL, dorsolateral ectoderm. (A) Intensity-rendered 3D reconstructions (see Movie 4 in the supplementary material); (B) transverse sections (see Movie 5 in the supplementary material). Cells at the dorsal edge protrude radially (white arrows) and move towards the ectoderm (yellow arrows indicate ventral cells protruding into the ectoderm). (C) DEC (white arrows) and more ventral cell (yellow arrows) protrusions visualised in a wild-type twi::CD2-expressing embryo fixed during mesoderm flattening (3D reconstructed image shown rotating in Movie 6 in the supplementary material). Embryo in C is shown in ventrolateral view, anterior towards the left.From: Clark IB, Muha V, Klingseisen A, Leptin M, Müller HA. Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila. Development. 2011 Jul;138:2705-15.
  • IF - Anti-Rat CD2 Antibody, clone OX-34 (Biotin) ABD12080
    Published customer image:Mouse anti Rat CD2 antibody, clone OX-34 used for the detection of rat CD2 tagged fusion protein in Drosophila by immunofluorescence.Image caption:A role for Cdc42 at the mesoderm/ectoderm interface. (A) Comparison of dorsal and radial protrusions (from twi::CD2 stainings) in wild-type embryos (yellow bars) and embryos expressing UAS::Cdc[N17] driven by twi::Gal4. The asterisks indicate statistically significant differences in the number of radial protrusions [P0.05 (n=5); two-sample t-test]. Data are mean±s.d. (B) Wild-type and Cdc42 mutant embryos (obtained from Cdc424/Cdc426 trans-heterozygous females) were stained for Twi (stages 8-10) and for Eve (stage 12). Note irregular distribution of mesoderm nuclei in Cdc42 mutants compared with wild type, indicating defects in mesoderm spreading. Arrowheads in stage 12 embryos indicate position of segmental Eve-positive dorsal mesoderm cells; note the strong reduction in the number of Eve-positive hemi-segments. (C) E-cadherin immunostaining of embryos (stage 9) expressing UAS::Cdc[N17] driven by twi::Gal4 compared with wild type. E-cadherin accumulates at the ectoderm-mesoderm interface (mesoderm is marked in red with CD2), which is absent in embryos expressing dominant-negative Cdc42.From: Clark IB, Muha V, Klingseisen A, Leptin M, Müller HA. Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila. Development. 2011 Jul;138:2705-15.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, FC
Primary Accession P08921
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names OX-34
Calculated MW 38414 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Activated rat T helper cells.
Shelf Life 18 months from date of despatch.
Gene ID 497761
Other Names T-cell surface antigen CD2, LFA-2, LFA-3 receptor, OX-34 antigen, T-cell surface antigen T11/Leu-5, CD2, Cd2
Target/Specificity Mouse anti-Rat CD2 antibody, clone OX-34 recognizes a determinant on thymocytes and peripheral T-cells but it does not bind to B cells or peritoneal macrophages. The antigen recognized by this antibody is a 50-54 kDa glycoprotein, homolog of the human CD2 antigen (Williamset al.1987).
Preservative & Stabilisers 0.09% Sodium Azide; 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Rat CD2 Antibody, clone OX-34 (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Williams, A.F. et al. (1987) Similarities in sequences and cellular expression between rat CD2 and CD4 antigens.
J Exp Med. 165 (2): 368-80. 2. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology. 42 (4): 593-600. 3. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. 4. Baker, S.C. et al. (2011) Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(e-caprolactone) Scaffold.
Macromol Biosci. 11: 618-27. 5. Romani, P. et al. (2009) Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform.
Genetics. 181: 165-75. 6. Stybayeva, G. et al. (2010) Lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function.
Anal Chem. 82: 3736-44. 7. Bastock, R. et al. (2003) Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning.
Development. 130: 3007-14. 8. Brückner, K. et al. (2000) Glycosyltransferase activity of Fringe modulates Notch-Delta interactions.
Nature. 406: 411-5. 9. Liversidge, J. et al. (2002) Nitric oxide mediates apoptosis through formation of peroxynitrite and Fas/Fas-ligand interactions in experimental autoimmune uveitis.
Am J Pathol. 160: 905-16. 10. Sarpal, R. et al. (2012) Mutational analysis supports a core role for Drosophila α-catenin in adherens junction function.
J Cell Sci. 125: 233-45. 11. Zhang, H. et al. (2011) Basic residues in the T-cell receptor ζ cytoplasmic domain mediate membrane association and modulate signaling.
Proc Natl Acad Sci U S A. 108: 19323-8. 12. Heck, B.W. et al. (2012) The transcriptional corepressor SMRTER influences both Notch and ecdysone signaling during Drosophiladevelopment.
Biol Open. 1 (3): 182-96. 13. Clark, I.B. et al. (2011) Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila.
Development. 138: 2705-15. 14. Domanitskaya, E. and Schüpbach, T. (2012) CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster.
J Cell Sci. 125: 399-410.

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