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Anti-Human Interleukin-2 Antibody, clone MQ1-17H12

Rat Anti-Human Monoclonal Antibody

  • E - Anti-Human Interleukin-2 Antibody, clone MQ1-17H12  ABD12081
    Sandwich ELISA analysis of human interleukin-2 using Rat anti Human IL-2 as a capture reagent and biotinylated Rabbit anti Human IL-2 as a detection reagent with recombinant human IL-2 as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). In the enlargement, neat serum (orange) and neat plasma (green) samples, low levels of human IL-2 (~1pg/ml) are detected
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P60568
Reactivity Human
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names MQ1-17H12
Calculated MW 17628 Da
Additional Information
Other Species Cynomolgus Pr
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Shelf Life 12 months from date of reconstitution.
Gene ID 3558
Other Names Interleukin-2, IL-2, T-cell growth factor, TCGF, Aldesleukin, IL2
Target/Specificity Rat anti-Human Interleukin-2 antibody, clone MQ1-17H12 recognizes human interleukin-2 (IL-2), also known as T-cell growth factor or Aldesleukin. IL-2 is a 153 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation.Rat anti-Human Interleukin-2 antibody, clone MQ1-17H12 has been sucessfully used for the detection and measurement of native and recombinant human IL-2 using flow cytometry and ELISA where it ascts as the capture reagent in a very sensitive assay for IL-2 determination in human samples. IL-2 is used extensively for the treatment of patients with metastatic melanoma (Atkinset al.1999) and renal cancer with repriducible benificial effects ( Monitoring of IL-2 levels in such patients is required for optimization of treatment regimens (Lotzeet al.1986). Rat anti-Human Interleukin-2 antibody, clone MQ1-17H12 is also useful for evaluating levels of IL-2 in patients with autoimmune conditions including lupus erythematosus and rheumatoid arthritis where there may be an impaired ability to produce IL-2 (Talalet al.1983).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Storage at 4℃ or at -20℃.
PrecautionsAnti-Human Interleukin-2 Antibody, clone MQ1-17H12 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Garbe, Y. et al. (2011) Semiallogenic fusions of MSI(+) tumor cells and activated B cells induce MSI-specific T cell responses.
BMC Cancer. 11: 410. 2. Moncrieffe, H. et al. (2010) High expression of the ectonucleotidase CD39 on T cells from the inflamed site identifies two distinct populations, one regulatory and one memory T cell population.
J Immunol. 185 (1): 134-43.

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Cat# ABD12081
(40 western blots)
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