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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Anti-PCNA Antibody, clone PC10    

Anti-PCNA Antibody, clone PC10

Mouse Anti-Rat Monoclonal Antibody

  • WB - Anti-PCNA Antibody, clone PC10  ABD12082
    K562 erythroleukemic whole cell lysate was run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Mouse anti Human PCNA was used as primary antibody followed by a Goat anti Mouse IgG (H/L):HRP secondary antibody at a dilution of 1/10,000 was used as the secondary antibody. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure in grey scale
  • WB - Anti-PCNA Antibody, clone PC10  ABD12082
    Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNA
  • FC - Anti-PCNA Antibody, clone PC10  ABD12082
    Staining of KM-H2 cells with Mouse anti PCNA:FITC permeabilised with Leucoperm and methanol
  • WB - Anti-PCNA Antibody, clone PC10  ABD12082
    K562 erythroleukemic whole cell lysate was run in lane 2 under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. ABD12082 Mouse anti Human PCNA:HRP conjugate was used as a direct label at a 1/1000 dilution. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure in grey scale
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04961
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names PC10
Calculated MW 28749 Da
Additional Information
Other Species Vertebrates,Invertebrates,Ferret,C,Rb,X,Rh,Ha,Atlantic Salmon,H,M,Hs,Sh,Dog,Cat,Cynomolgus Pr
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Rat PCNA made in the protein A expression vector pR1T2T
Shelf Life 18 months from date of despatch.
Gene ID 25737
Other Names Proliferating cell nuclear antigen, PCNA, Cyclin, Pcna
Target/Specificity Mouse anti-PCNA antibody, clone PC10 recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Liet al.1995) through its interaction with FEN1(Wuet al.1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravoet al.1987).PCNA is highly conserved between mammalian species and other vertebrates. Clone PC10 has been used for the detection of PCNA in a number of species including human, rat (Elsässeret al.1994), mouse (Parket al.2008), chicken (Franz-Odendaal 2008) and abalone (Harriset al.2006).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-PCNA Antibody, clone PC10 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Matthews, M. B. et al. (1984) Identity of the proliferating cell nuclear antigen and cyclin.
Nature 309: 374-376. 2. Ogata, K. et al. (1985) Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies.
J. Immunol. 135: 2623-2627. 3. Garcia, R. L. et al. (1989) Analysis of proliferation grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues compared with flow cytometry.
Am. J. Pathol. 134: 733-739. 4. Landberg, G. et al. (1990) Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and ki-67 antigen: a new view of the cell cycle.
Exp. Cell. Res. 187: 111-118. 5. Wilson, G. D. et al. (1992) Flow cytometric characterisation of proliferating cell nuclear antigen using the monoclonal antibody PC10.
Eur. J. Cancer 28A: 2010-2017. 6. Prosperi, E. et al. (1993) Proliferating cell nuclear antigen complex formation induced by ultra violet irradiation in human quiescent fibroblasts as detected by immunostaining and flow cytometry.
Exp. Cell Res. 205: 320 - 325. 7. Elsasser, H. P. et al. (1994) Growth of rat pancreatic acinar cells quantitated with a monoclonal antibody against the proliferating cell nuclear antigen.
Cell Tissue Res. 276: 603 - 609. 8. Harris, L. et al. (2006) Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniques
Aquaculture 261: 1413-21 9. Buggins, A.G. et al. (2001) Microenvironment produced by acute myeloid leukemia cells prevents T cell activation and proliferation by inhibition of NF-kappaB, c-Myc, and pRb pathways.
J Immunol. 167: 6021-30. 10. Kapitonova, M.Y. et al. (2010) Immunohistochemical characteristics of the hypophysis in normal conditions and chronic stress.
Neurosci Behav Physiol. 40: 97-102. 11. Franz-Odendaal, T.A. (2008) Toward understanding the development of scleral ossicles in the chicken, Gallus gallus.
Dev Dyn. 237: 3240-51. 12. Hashimoto, Y. et al. (2010) Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis.
Nat Struct Mol Biol. 17: 1305-11. 13. Jenkins, H. et al. (1993) Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix.
J Cell Sci. 106: 275-85. 14. Park, J.H. et al. (2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.
J Comp Pathol. 139: 208-17. 15. Izhak, L. et al. (2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.
PLoS One. 7: e28305.

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Cat# ABD12082
(40 western blots)
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