|Application ||WB, IHC-P, IHC-F, FC, IP|
|Calculated MW||28749 Da|
|Other Species||Vertebrates,Invertebrates,Ferret,C,Rb,X,Rh,Ha,Atlantic Salmon,H,M,Hs,Sh,Dog,Cat,Cynomolgus Pr|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Rat PCNA made in the protein A expression vector pR1T2T|
|Shelf Life||18 months from date of despatch.|
|Other Names||Proliferating cell nuclear antigen, PCNA, Cyclin, Pcna|
|Target/Specificity||Mouse anti-PCNA antibody, clone PC10 recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Liet al.1995) through its interaction with FEN1(Wuet al.1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravoet al.1987).PCNA is highly conserved between mammalian species and other vertebrates. Clone PC10 has been used for the detection of PCNA in a number of species including human, rat (Elsässeret al.1994), mouse (Parket al.2008), chicken (Franz-Odendaal 2008) and abalone (Harriset al.2006).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-PCNA Antibody, clone PC10 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Matthews, M. B. et al. (1984) Identity of the proliferating cell nuclear antigen and cyclin.Nature 309: 374-376. 2. Ogata, K. et al. (1985) Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies.J. Immunol. 135: 2623-2627. 3. Garcia, R. L. et al. (1989) Analysis of proliferation grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues compared with flow cytometry.Am. J. Pathol. 134: 733-739. 4. Landberg, G. et al. (1990) Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and ki-67 antigen: a new view of the cell cycle.Exp. Cell. Res. 187: 111-118. 5. Wilson, G. D. et al. (1992) Flow cytometric characterisation of proliferating cell nuclear antigen using the monoclonal antibody PC10.Eur. J. Cancer 28A: 2010-2017. 6. Prosperi, E. et al. (1993) Proliferating cell nuclear antigen complex formation induced by ultra violet irradiation in human quiescent fibroblasts as detected by immunostaining and flow cytometry. Exp. Cell Res. 205: 320 - 325. 7. Elsasser, H. P. et al. (1994) Growth of rat pancreatic acinar cells quantitated with a monoclonal antibody against the proliferating cell nuclear antigen. Cell Tissue Res. 276: 603 - 609. 8. Harris, L. et al. (2006) Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniquesAquaculture 261: 1413-21 9. Buggins, A.G. et al. (2001) Microenvironment produced by acute myeloid leukemia cells prevents T cell activation and proliferation by inhibition of NF-kappaB, c-Myc, and pRb pathways.J Immunol. 167: 6021-30. 10. Kapitonova, M.Y. et al. (2010) Immunohistochemical characteristics of the hypophysis in normal conditions and chronic stress.Neurosci Behav Physiol. 40: 97-102. 11. Franz-Odendaal, T.A. (2008) Toward understanding the development of scleral ossicles in the chicken, Gallus gallus.Dev Dyn. 237: 3240-51. 12. Hashimoto, Y. et al. (2010) Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis.Nat Struct Mol Biol. 17: 1305-11. 13. Jenkins, H. et al. (1993) Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix.J Cell Sci. 106: 275-85. 14. Park, J.H. et al. (2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.J Comp Pathol. 139: 208-17. 15. Izhak, L. et al. (2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.PLoS One. 7: e28305.
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