|Application ||WB, FC, E|
|Calculated MW||40076 Da|
|Other Species||Dog,Ga,Cat,Rb,Mink,B,Pig,Sh,Cynomolgus Pr,Llama|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Other Names||Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14|
|Target/Specificity||Mouse anti-human CD14 antibody, clone TÜK4 recognizes the human CD14 cell surface antigen. CD14 is a 55 KDa glycoprotein that contains multiple leucin-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage (Simmonset al.1989) but a soluble form of CD14 also exists (Bazilet al.1986).CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells (Jersmann 2005). CD14 functions as a pattern recognition receptor (Puginet al.1994,Dziarskiet al.1998) in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria.Mouse anti-human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner (Yanget al.2003). Serotec recommend the use of the anti-human CD14 antibody, Low Endotoxin format for this purpose.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD14 Antibody, clone TÜK4 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Soell, M. et al. (1995) Activation of human monocytes by streptococcal rhamnose glucose polymers is mediated by CD14 antigen, and mannan binding protein inhibits TNF-alpha release.J Immunol. 154 (2): 851-60. 2. Gupta, V.K. et al. (1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.Vet Immunol Immunopathol. 51 (1-2): 89-99. 3. Sopp, P. & Howard, C.J. (1997) Cross-reactivity of monoclonal antibodies to defined human leucocyte differentiation antigens with bovine cells.Vet Immunol Immunopathol. 56 (1-2): 11-25. 4. Willett, B.J. et al. (2003) Expression of CXCR4 on feline peripheral blood mononuclear cells: effect of feline immunodeficiency virus infection.J Virol. 77 (1): 709-12. 5. Werling, D. et al. (1998) Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus.Vet Immunol Immunopathol. 62 (3): 185-95. 6. Yang, H. et al. (2003) Antibody to CD14 like CXCR4-specific antibody 12G5 could inhibit CXCR4-dependent chemotaxis and HIV Env-mediated cell fusion.Immunol Lett. 88 (1): 27-30. 7. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110. 8. Jacobsen, C.N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.Vet Immunol Immunopathol. 39 (4): 461-6. 9. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.Vet Immunol Immunopathol. 132:109-15. 10. Dalli J et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.Blood. 112 (6): 2512-9. 11. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium aviumsubsp.paratuberculosis-infected goats.Clin. Vaccine. Immunol. 16: 1003-11. 12. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.PLoS One. 5: e13705. 13. Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.J Virol. 80: 7873-84. 14. Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.J Virol. 81: 9665-79. 15. Kallapur, S.G. et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95.2. Simmons, D. L. et al. (1989) Monocyte antigen CD14 is a phospholipid anchored membrane protein.Blood. 73:284-9.3. Bazil, V. et al. (1986) Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.Eur J Immunol. 16:1583-9.4. Jersmann, H.P. (2005) Time to abandon dogma: CD14 is expressed by non-myeloid lineage cells.Immunol Cell Biol. 83:462-7.5. Pugin, J. et al. (1994) CD14 is a pattern recognition receptor.Immunity.1:509-16.6. Dziarski, R. et al. (1998) Binding of bacterial peptidoglycan to CD14.J Biol Chem. 273:8680-90.16. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.Vet Res. 39: 54.
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