|Application ||IHC-F, FC|
|Calculated MW||40716 Da|
|Other Species||Cynomolgus Pr|
|Purification||Purified IgG prepared by affinity chromatography on Protein A.|
|Shelf Life||6 months from date of reconstitution.|
|Other Names||Hematopoietic progenitor cell antigen CD34, CD34, CD34|
|Target/Specificity||Mouse anti-Human CD34 antibody, clone 581 recognizes the human CD34 cell surface antigen also known as Hematopoietic progenitor cell antigen CD34. Clone 581 binds to an epitope of human CD34 resistant to neuraminadase, chymopapain and glycoprotease treatments. The binding epitopes are classified according to their resistance to enzymatic degradation and antibodies assigned according to this and cross inhibition studies. Clone 581 is classified as class III (Lanzaet al.1999).CD34 is a 385 amino acid pro-peptide with the 31 amino acid signal peptide cleaved to yield the mature form of CD34. CD34 is a single pass type I ~105-120 kD highly glycosylated transmembrane glycoprotein, expressed by hematopoietic stem cells and small vessel endothelial cells. CD34 contains a number of sialylated residues and has a mucin-like structure (Simmonset al.1992). A truncated form of the CD34 molecule has also been identified lacking the carboxy-terminal region of the intracellular sequence, also bearing a sequence switch in the remaining cytoplasmic region (Nakamuraet al.1993). Clone 581 is expected to react with both isoforms of the CD34 molecule as it recognizes an epitope located in the extracellular region of human CD34.Mouse anti-Human CD34 antibody, clone 581 has been used successfully for the immunohistochemical detection of stem-like cells present in hepatoblastoma lesions on cryotat sections (Fiegalet al.2004).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD34 Antibody, clone 581 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Civin, C.I. et al. (1984) Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.J Immunol. 133 (1): 157-65. 2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49: 97-110. 3. Maumus, M. et al. (2011) Native human adipose stromal cells: localization, morphology and phenotype.Int J Obes (Lond). 35 (9): 1141-53. 4. Fiegel, H.C. et al. (2004) Stem-like cells in human hepatoblastoma.J Histochem Cytochem. 52 (11): 1495-501.
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