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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD34 Antibody, clone 581 (RPE)   

Anti-Human CD34 Antibody, clone 581

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P28906
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 581
Calculated MW 40716 Da
Additional Information
Other Species Cynomolgus Pr
Purification Purified IgG prepared by affinity chromatography on Protein A.
Shelf Life 6 months from date of reconstitution.
Gene ID 947
Other Names Hematopoietic progenitor cell antigen CD34, CD34, CD34
Target/Specificity Mouse anti-Human CD34 antibody, clone 581 recognizes the human CD34 cell surface antigen also known as Hematopoietic progenitor cell antigen CD34. Clone 581 binds to an epitope of human CD34 resistant to neuraminadase, chymopapain and glycoprotease treatments. The binding epitopes are classified according to their resistance to enzymatic degradation and antibodies assigned according to this and cross inhibition studies. Clone 581 is classified as class III (Lanzaet al.1999).CD34 is a 385 amino acid pro-peptide with the 31 amino acid signal peptide cleaved to yield the mature form of CD34. CD34 is a single pass type I ~105-120 kD highly glycosylated transmembrane glycoprotein, expressed by hematopoietic stem cells and small vessel endothelial cells. CD34 contains a number of sialylated residues and has a mucin-like structure (Simmonset al.1992). A truncated form of the CD34 molecule has also been identified lacking the carboxy-terminal region of the intracellular sequence, also bearing a sequence switch in the remaining cytoplasmic region (Nakamuraet al.1993). Clone 581 is expected to react with both isoforms of the CD34 molecule as it recognizes an epitope located in the extracellular region of human CD34.Mouse anti-Human CD34 antibody, clone 581 has been used successfully for the immunohistochemical detection of stem-like cells present in hepatoblastoma lesions on cryotat sections (Fiegalet al.2004).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD34 Antibody, clone 581 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Civin, C.I. et al. (1984) Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.
J Immunol. 133 (1): 157-65. 2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Exp. Anim. 49: 97-110. 3. Maumus, M. et al. (2011) Native human adipose stromal cells: localization, morphology and phenotype.
Int J Obes (Lond). 35 (9): 1141-53. 4. Fiegel, H.C. et al. (2004) Stem-like cells in human hepatoblastoma.
J Histochem Cytochem. 52 (11): 1495-501.

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Cat# ABD12086
(40 western blots)
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