|Calculated MW||22517 Da|
|Purification||Purified IgG prepared by affinity chromatography|
|Immunogen||Murine dendritic epidermal Y245 cell line|
|Shelf Life||6 months from date of reconstitution.|
|Other Names||Early activation antigen CD69, CD69, Cd69|
|Target/Specificity||Hamster anti-Mouse CD69 antibody, clone H1.2F3 recognizes the murine CD69 cell surface antigen, an 85kDa dimeric glycoprotein. CD69 is expressed rapidly on the surface of T cells, B cells and NK cells following activation.|
|Preservative & Stabilisers||0.09% Sodium Azide; Stabilizing agent|
|Storage||Storage at +4ºC. DO NOT FREEZE.|
|Precautions||Anti-Mouse CD69 Antibody, clone H1.2F3 (RPE-CY5) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Yokoyama,W.M. et al. (1988) Characterization of a cell surface - expressed disulfide-linked dimer involved in murine T cell activation.J. Immunol. 141: 369-376. 2. Hickman, H.D. et al. (2008) Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes.Nat Immunol. 9: 155-65. 3. Chalifour, A. et al. (2004) Direct bacterial protein PAMP recognition by human NK cells involves TLRs and triggers alpha-defensin production.Blood. 104: 1778-83. 4. Cohen-Sfady, M. et al. (2005) Heat shock protein 60 activates B cells via the TLR4-MyD88 pathway.J Immunol. 175: 3594-602. 5. Iwashiro, M. et al. (2001) Immunosuppression by CD4+ regulatory T cells induced by chronic retroviral infection.Proc Natl Acad Sci U S A. 98: 9226-30. 6. Karrer, U. et al. (2003) Memory inflation: continuous accumulation of antiviral CD8+ T cells over time.J Immunol. 170: 2022-9. 7. Kvakan, H. et al. (2009) Regulatory T cells ameliorate angiotensin II-induced cardiac damage.Circulation. 119: 2904-12. 8. Marshall-Clarke, S. et al. (2003) A differential requirement for phosphoinositide 3-kinase reveals two pathways for inducible upregulation of major histocompatibility complex class II molecules and CD86 expression by murine B lymphocytes.Immunology. 109: 102-8. 9. Zajac, A.J. et al. (1998) Viral immune evasion due to persistence of activated T cells without effector function.J Exp Med. 188: 2205-13. 10. Everitt, A.R. et al. (2012) IFITM3 restricts the morbidity and mortality associated with influenza.Nature. 484: 519-23.
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