|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Bovine CD205 antibody, clone CC98 recognizes the bovine CD205 cell surface antigen, a 210-220kDa molecule expressed by T cells that are CD2+ve but not WC1+ve. CD205 is also expressed by B cells, and weakly stains B cell follicles.Bovine CD205 has previously been described as the WC6 antigen (Gliddonet al.2004).Dendritic cells (veiled cells) in afferent lymph are strong expressors of CD205 as are dendritic cells in various other tissues.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin|
|Storage||Store at +4℃ or -20℃.|
|Precautions||Anti-Bovine CD205 Antibody, clone CC98 (FITC) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).Vet Immunol Immunopathol. 39 (1-3): 25-47. 2. Howard, C.J. et al. (1996) Afferent lymph veiled cells stimulate proliferative responses in allogeneic CD4+ and CD8+ T cells but not gamma delta TCR+ T cells.Immunology. 88 (4): 558-64. 3. Naessens, J. et al. (1993) Cross-reactivity of workshop antibodies with cells from domestic and wild ruminants.Vet Immunol Immunopathol. 39 (1-3): 283-90. 4. Gliddon, D.R. et al. (2004) DEC-205 expression on migrating dendritic cells in afferent lymph.Immunology. 111 (3): 262-72. 5. Akesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.Dev Comp Immunol. 32: 837-49. 6. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.Vet Res. 42:19. 7. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.PLoS One. 5: e13705. 8. Fach, S.J. et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.Viral Immunol. 20: 119-30. 9. Eicher, S.D. et al. (2011) β-Glucan plus ascorbic acid in neonatal calves modulates immune functions with and without Salmonella enterica serovar Dublin.Vet Immunol Immunopathol. 142: 258-64. 10. Olivier, M. et al. (2012) Capacities of Migrating CD1b Lymph Dendritic Cells to Present Salmonella Antigens to Naive T CellsPLoS One. 7: e30430. 11. Thonur, L. et al. (2012) Toll-like receptor gene expression in fresh and archived ovine pseudoafferent lymph DEC205+ dendritic cells.J Comp Pathol. 147 (2-3): 296-304. 12. Sigmundsdottir, H. et al. (2007) DCs metabolize sunlight-induced vitamin D3 to 'program' T cell attraction to the epidermal chemokine CCL27.Nat Immunol. 8: 285-93. 13. Fach, S.J. et al. (2007) Neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (IL)-4 And IL-10 gene transcripts.Viral Immunol. 20: 119-30. 14. McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.J Histochem Cytochem. 54: 1021-30. 15. Walters, A.A. et al. (2015) Assessment of the enhancement of PLGA nanoparticle uptake by dendritic cells through the addition of natural receptor ligands and monoclonal antibody.Vaccine. pii: S0264-410X(15)01549-2.
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