Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Anti-Bovine CD4 Antibody, clone CC8 (RPE)   

Anti-Bovine CD4 Antibody, clone CC8

Mouse Anti-Bovine Monoclonal Antibody

     
  • FC - Anti-Bovine CD4 Antibody, clone CC8  ABD12094
    Published customer image:Mouse anti Bovine CD4 antibody, clone CC8 used for the identification of CD4 expressing lymphocytes in preipheral blood by flow cytometry.Image caption:Identification of multifunctional IFN-γ, IL-2 and TNF-α CD4+ cells in natural M. bovis infection. PBMC from naturally M. bovis infected cattle were cultured in the presence of either PPD-B or medium and the co-expression of IFN-γ, IL-2 and TNF-α determined by ICS flow cytometry. Histograms were gated on singlet, live lymphocytes and then all CD4+ cells analysed for all combinations of cytokine productivity. The upper histograms show the total proportion of CD4+ cells staining for expression of IFN-γ, IL-2 or TNF-α following stimulation with PPD-B (left panel) or medium control (right panel). Subgating of the IFN-γ+ and IFN-γ- CD4+ cells provides histograms that represent all possible functionalities for the expressing of the 3 cytokines, as shown in the lower histograms. The numbers indicate percentage of CD4+ cells and data are representative of 1 of 10 naturally infected cattle.From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.PLoS ONE 6: e29194.
  • FC - Anti-Bovine CD4 Antibody, clone CC8  ABD12094
    Published customer image:Mouse anti Bovine CD4 antibody, clone CC8 used for the identification of CD4 expressing lymphocytes in preipheral blood by flow cytometry.Image caption:Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN-γ within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN-γ and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments.From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.PLoS ONE 6: e29194.
  • FC - Anti-Bovine CD4 Antibody, clone CC8  ABD12094
    Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD4:Alexa Fluor® 647
  • FC - Anti-Bovine CD4 Antibody, clone CC8  ABD12094
    Published customer image:Mouse anti Bovine CD4 antibody, clone CC8 used for the evaluation of CD4 expression on proliferating bovine peripheral blood mononuclear cells by flow cytometry.Image caption:Determination of phenotypes of proliferating PBMC by FACS analysis. Determination of phenotypes (CD4+, CD8+ and WC1+) of proliferating PBMC by CFDA SE staining after re-stimulating with respective vaccine antigen and fluorescence-activated cell sorter analysis. PBMC were separated from heparinised blood of vaccinated cattle originated from full dose vaccine group on 4 weeks after homologous FMDV challenge. Two naïve cattle without FMDV infection and two A Malaysia 97 FMDV infected cattle (4 weeks post-infection) without prior vaccination were included as control group. PBMCs from naïve animals were re-stimulated with SAT2 Eritrea vaccine antigen. FACS plots were exemplified only for one vaccinated challenged animal from SAT2 Eritrea animal experiment. Similar FACS plots were obtained for the PBMC originated from A Malaysia 97 and SAT2 Eritrea vaccinated animals. Error bars represent standard error of the mean.From: Oh Y, Fleming L, Statham B, Hamblin P, Barnett P, Paton DJ, et al. Interferon-γ Induced by In Vitro Re-Stimulation of CD4+ T-Cells Correlates with In Vivo FMD Vaccine Induced Protection of Cattle against Disease and Persistent Infection.PLoS ONE 7(9): e44365.
  • FC - Anti-Bovine CD4 Antibody, clone CC8  ABD12094
    Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD4:FITC
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, IF, FC, IP
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names CC8
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Bovine lymphocytes.
Shelf Life 12 months from date of reconstitution.
Target/Specificity Mouse anti-Bovine CD4 antibody, clone CC8 recognizes bovine CD4, the homolog of human CD4 and immunoprecipitates a ~50 kDa molecule. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Clone CC8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Eskraet al.1991).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Bovine CD4 Antibody, clone CC8 is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.

References

1. Bensaid, A. & Hadam, M. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4).
Vet Immunol Immunopathol. 27 (1-3): 51-4. 2. Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets.
Vet Immunol Immunopathol. 27 (1-3): 215-22. 3. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
Vet Immunol Immunopathol. 27 (1-3): 21-7. 4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.
Vet Immunol Immunopathol. 71 (3-4): 321-34. 5. Sidders, B. et al. (2008) Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosiscomplex.
Infect Immun. 76: 3932-9. 6. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitroandin vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. 7. Buddle, B.M. et al. (2003) Revaccination of neonatal calves with Mycobacterium bovis BCG reduces the level of protection against bovine tuberculosis induced by a single vaccination.
Infect Immun. 71: 6411-9. 8. Gerner, W. et al. (2009) Identification of major histocompatibility complex restriction and anchor residues of foot-and-mouth disease virus-derived bovine T-cell epitopes.
J Virol. 83: 4039-50. 9. Harris, J. et al. (2002) Expression of caveolin by bovine lymphocytes and antigen-presenting cells
Immunology. 105: 190-5. 10. Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves.
BMC Vet Res. 6: 39. 11. Hu, X.D. et al. (2009) Immunotherapy with combined DNA vaccines is an effective treatment for M. bovis infection in cattle
Vaccine. 27: 1317-22. 12. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
Vet Res. 41: 14. 13. Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS One. 6: e29194. 14. Wernike, K. et al. (2013) Oral exposure, reinfection and cellular immunity to Schmallenberg virus in cattle.
Vet Microbiol. pii: S0378-1135(13)00092-8. 15. Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving.
Vet Res Commun. 34: 197-203. 16. Dacal,. V. et al. (2013) Immunohistochemical characterization of inflammatory cells in the skin of cattle undergoing repeated infestations with Hypoderma lineatum (Diptera: Oestridae) larvae.
J Comp Pathol. 145: 282-8. 17. Oh, Y. et al. (2012) Interferon-γ induced by in vitro re-stimulation of CD4+ T-cells correlates with in vivo FMD vaccine induced protection of cattle against disease and persistent infection.
PLoS One. 7: e44365. 18. Hine, B.C. et al. (2012) Analysis of leukocyte populations in Canadian Holsteins classified as high or low immune responders for antibody- or cell-mediated immune response.
Can J Vet Res. 76: 149-56. 19. Aranday-Cortes, E. et al. (2012) Transcriptional profiling of disease-induced host responses in bovine tuberculosis and the identification of potential diagnostic biomarkers.
PLoS One. 7: e30626. 20. Tenaya, I.W. et al. (2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.
Vet Immunol Immunopathol. 149: 167-76. 21. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.
Theriogenology. 82 (3): 403-10.

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Cat# ABD12094
Size:
Quantity:
Availability: Inquire
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Crown Flash17-30% Off
Terms & Conditions