|Application ||IHC-F, IF, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant|
|Shelf Life||12 months from date of reconstitution.|
|Target/Specificity||Mouse anti-Bovine CD4 antibody, clone CC8 recognizes bovine CD4, the homolog of human CD4 and immunoprecipitates a ~50 kDa molecule. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Clone CC8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Eskraet al.1991).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bovine CD4 Antibody, clone CC8 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Bensaid, A. & Hadam, M. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4).Vet Immunol Immunopathol. 27 (1-3): 51-4. 2. Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets.Vet Immunol Immunopathol. 27 (1-3): 215-22. 3. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.Vet Immunol Immunopathol. 27 (1-3): 21-7. 4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques.Vet Immunol Immunopathol. 71 (3-4): 321-34. 5. Sidders, B. et al. (2008) Screening of highly expressed mycobacterial genes identifies Rv3615c as a useful differential diagnostic antigen for the Mycobacterium tuberculosiscomplex.Infect Immun. 76: 3932-9. 6. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitroandin vivo in response to noncytopathic bovine viral diarrhea virus.J Virol. 79: 7738-44. 7. Buddle, B.M. et al. (2003) Revaccination of neonatal calves with Mycobacterium bovis BCG reduces the level of protection against bovine tuberculosis induced by a single vaccination.Infect Immun. 71: 6411-9. 8. Gerner, W. et al. (2009) Identification of major histocompatibility complex restriction and anchor residues of foot-and-mouth disease virus-derived bovine T-cell epitopes.J Virol. 83: 4039-50. 9. Harris, J. et al. (2002) Expression of caveolin by bovine lymphocytes and antigen-presenting cellsImmunology. 105: 190-5. 10. Lynch, E.M. et al. (2010) Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves.BMC Vet Res. 6: 39. 11. Hu, X.D. et al. (2009) Immunotherapy with combined DNA vaccines is an effective treatment for M. bovis infection in cattleVaccine. 27: 1317-22. 12. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.Vet Res. 41: 14. 13. Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.PLoS One. 6: e29194. 14. Wernike, K. et al. (2013) Oral exposure, reinfection and cellular immunity to Schmallenberg virus in cattle.Vet Microbiol. pii: S0378-1135(13)00092-8. 15. Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving.Vet Res Commun. 34: 197-203. 16. Dacal,. V. et al. (2013) Immunohistochemical characterization of inflammatory cells in the skin of cattle undergoing repeated infestations with Hypoderma lineatum (Diptera: Oestridae) larvae.J Comp Pathol. 145: 282-8. 17. Oh, Y. et al. (2012) Interferon-γ induced by in vitro re-stimulation of CD4+ T-cells correlates with in vivo FMD vaccine induced protection of cattle against disease and persistent infection.PLoS One. 7: e44365. 18. Hine, B.C. et al. (2012) Analysis of leukocyte populations in Canadian Holsteins classified as high or low immune responders for antibody- or cell-mediated immune response.Can J Vet Res. 76: 149-56. 19. Aranday-Cortes, E. et al. (2012) Transcriptional profiling of disease-induced host responses in bovine tuberculosis and the identification of potential diagnostic biomarkers.PLoS One. 7: e30626. 20. Tenaya, I.W. et al. (2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.Vet Immunol Immunopathol. 149: 167-76. 21. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis.Theriogenology. 82 (3): 403-10.
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