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Mouse Anti-Bovine Monoclonal Antibody
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- BACKGROUND
Application 
| FC, IP |
|---|---|
| Reactivity | Bovine |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone Names | CC58 |
| Other Species | Sh,Ga,Water Buffalo |
|---|---|
| Purification | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Shelf Life | 12 months from date of reconstitution. |
| Target/Specificity | Mouse anti-Bovine CD8 beta antibody, clone CC58 recognizes an epitiope associated with the bovine CD8 beta chain. CD8 is usually expressed as an α/β heterodimer. Mouse anti-Bovine CD8 beta antibody, clone CC58 has been successfully used for the immunohistochemical detection of CD8 on formalin fixed, paraffin embedded placental tissue from water buffalo (Cantónet al.2014). |
| Preservative & Stabilisers | 0.09% Sodium Azide (NaN3) |
| Storage | Storage at 4℃ or at -20℃. |
| Precautions | Anti-Bovine CD8 beta Antibody, clone CC58 is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
References
1. Suraud, V. et al. (2008) Acute infection by conjunctival route with Brucella melitensis induces IgG+ cells and IFN-gamma producing cells in peripheral and mucosal lymph nodes in sheep.Microbes Infect. 10: 1370-8. 2. Howard, C.J. and Naessens, J. (1993) Summary of workshop findings for cattle.Vet. Immunol. Immunopathol. 39: 25-48. 3. Naessens, J. et al. (1997) Nomenclature and characterization of leukocyte differentiation antigens in ruminants.Immunology Today 18(8): 365-368. 4. Hein, W. R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep. Vet. Immunol. Immunopathol. 27: 28-30. 5. Gerner, W. et al. (2009) Identification of major histocompatibility complex restriction and anchor residues of foot-and-mouth disease virus-derived bovine T-cell epitopes.J Virol. 83: 4039-50. 6. Gerner, W. et al. (2010) Sensitive detection of Foxp3 expression in bovine lymphocytes by flow cytometry.Vet Immunol Immunopathol. 138: 154-8. 7. MacHugh, N.D. and Sopp, P. (1991) Individual antigens of cattle. Bovine CD8 (BoCD8).Vet Immunol Immunopathol. 27: 65-9. 8. Soltys, J. and Quinn, M.T. (1999) Selective recruitment of T-cell subsets to the udder during staphylococcal and streptococcal mastitis: analysis of lymphocyte subsets and adhesion molecule expression.Infect Immun. 67: 6293-302. 9. Cantón, G.J. et al. (2014) Characterization of immune cell infiltration in the placentome of water buffaloes (Bubalus bubalis) infected with neospora caninum during pregnancy.J Comp Pathol. 150: 463-8.
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